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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 431-439.doi: 10.3724/SP.J.1006.2013.00431

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transformation of Antisense Wheat Mlo (Ta-Mlo) Gene and Wheat Powdery Mildew Resistance Analysis of Transgenic Plants

XING Li-Ping,QIAN Chen,LI Ming-Hao,CAO Ai-Zhong,WANG Xiu-E,CHEN Pei-Du*   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-08-01 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 陈佩度, E-mail: pdchen@njau.edu.cn

Abstract:

The antisense wheatMlo gene, Ta-Mlo, was transformed into wheat (Tritivum aestivum L.) varieties Yangmai 158 and Jimai 20 via biolistic transformation using immature embryo calli as explants. After two rounds of bialaphos selection and regeneration, herbicide-resistant plants were obtained, which were subsequently confirmed by PCR, PCR-Southern hybridization, genomic dot hybridization, and BASTA resistance analysis. The results showed that the Ta-Mlo antisense transgenic Yangmai 158 and Jimai 20 plants were obtained. The real time fluorescence quantitative PCR analysis proved that the transcript of Ta-Mlo was knocked down in these transgenic plants. The disease resistance test showed that the six transgenic lines appeared highly resistance to powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt). The transgenic lines showed distinct acceleration of the production and stabilization of papillae, and effective suppression to further development of haustoria of Bgt. Therefore, the transgenic lines showed high resistance to Bgt.

Key words: Biolistic transformation, Antisense wheat Mlo gene, Wheat powdery mildew, Papillae and haustoria

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