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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 1013-1020.doi: 10.3724/SP.J.1006.2013.01013

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of the D2 Gene in Rice

IN Yan-Ling1,2,YIN Liang3,ZHAO Jin-Feng2,SUN Wei3,ZHAO Qing-Lei3,YUAN Shou-Jiang3,ZHU Wen-Yin3,GUO Bao-Tai1,*,LI Xue-Yong2,*   

  1. 1 College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shandong Rice Research Institute, Jinan 250100, China
  • Received:2012-11-13 Revised:2013-01-18 Online:2013-06-12 Published:2013-03-22
  • Contact: 郭宝太, E-mail: btguo12@163.com, Tel: 15969841765; 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409

Abstract:

Two dwarf mutants s1-46 and s1-96 were isolated from an EMS mutated japonica cultivar Nipponbare. The plant height of the two mutants was 44.7% and 55.9% of that of the wild-type plant, respectively. The mutants also exhibited erect leaves, reduced grain number,,and shortened grains. When grown in complete darkness, the mesocotyl elongated in the wild-type plant, but not in the mutants, indicating that s1-46 and s1-96 are brassinosteroids (BRs)-related mutants. Furthermore, treatment of seedling with bioactive BR showed that the leaf angle was increased and the root length was decreased in both the wild-type and mutant plants, suggesting that the mutated gene is involved in BR biosynthesis. Genetic analysis showed that the phenotypes of both mutants were caused by a recessive gene. Fine-mapping showed that this gene was located within a 40.9 kb region on chromosome 1. Sequence analysis revealed that the mutated gene was allelic to D2, encoding CYP90D2that is involved in BR biosynthesis. In s1-46 and s1-96, the encoded amino acids were changed from proline to leucine and from glycine to glutamic acid at the 305th and370thpositions of D2, respectively.

Key words: Rice, Dwarf mutant, BR, Fine-mapping

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