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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1360-1365.doi: 10.3724/SP.J.1006.2013.01360

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Aspergillus niger phyA2 Transgenic Maize on Utilization of Organic Phosphorus in Soil

HOU Wen-Tong1,YANG Li-Ping1,*,CHEN Ru-Mei2,ZHANG Shao-Jun2   

  1. 1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-06 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 杨俐苹, E-mail: yangliping@caas.cn, Tel: 010-82105030

Abstract:

Homozygous phyA2 transgenic maize lines at T9 generation and the corresponding generation of negative control were grown in low-phosphorus (P) soil to investigate the ability of maize plant to acquire P from organic sources. It showed that the soil phosphatase activity increased by 5.17% and 5.48%, the moderately labile organic P decreased by 16.2% and 28.2% respectively in phyA2 transgenic maize rhizosphere, and the P accumulation of transgenic plant increased significantly by 140% and 100% respectively as compared with a control plant line when supplied with phytate as P sources and without P-fertilizer. The plant growth of phyA2 transgenic maize was better improved than that of the negative control plant. These data indicated that we can improve the ability of plant to utilize soil organic P, the plant P accumulation and the plant growth by transferring Aspergillus niger phyA2 gene into maize.

Key words: Phytase gene (phyA2), Maize, Soil organic phosphorus, Plant phosphorus

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