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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1386-1391.doi: 10.3724/SP.J.1006.2014.01386

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Quantitative Traits Loci Analysis of Seed Glucosinolate Content in Brassica napus Using High-density SNP Map

JIAN Hong-Ju,WEI Li-Juan,LI Jia-Na,XU Xin-Fu,CHEN Li,LIU Lie-Zhao*   

  1. Chongqing Engineering Research Center for Rapeseed / College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
  • Received:2014-02-15 Revised:2014-04-16 Online:2014-08-12 Published:2014-06-03
  • Supported by:

    This research was supported by The National Natural Science Foundation of China (No. 31171584), The Chongqing Natural Science Foundation (No.cstc2011jjA8000g), and The Program of Introducing International Super Agricultural Science and Technology (948 Program) (No.2011-G23).

Abstract:

Seed glucosinolate plays important biological and economic roles in Brassica napus. In this study, we aimed at identifying QTLs related to seed glucosinolate content of B. napus using the composite interval mapping (CIM) method based on the high density SNP genetic map. The total seed glucosinolate content was analyzed via Near Infrared Spectroscopy (NIR) using standard methods with three technical replicates. The QTLs related to seed glucosinolate content in two years were detected using the SNP genetic map constructed in 2013, which contains 2795 SNP markers with the total map length of 1832.9 cM and an average distance of 0.66 cM. Five QTLs for seed total glucosinolate content were identified on A03, A09, C02 in both 2011 and 2012, and LOD threshold values for significant QTLs both in 2011 and in 2012 were determined to be 2.90–10.4. These QTLs explained for 56.9% and 55.1% of the total phenotypic variance in 2011 and 2012, respectively. Another five minor QTLs were also detected either in 2011 or 2012. These QTLs accounted for 4.1%–7.9% of the phenotypic variance and the LOD threshold values were 2.53–3.83.

Key words: Brassica napus, Single nucleotide polymorphism, Quantitative trait loci, Seed glucosinolate content

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