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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1702-1709.doi: 10.3724/SP.J.1006.2014.01702

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles    

Molecular Cloning and Expression of a bZIP Transcription Factor Gene CsbZIP1 in Tea Plant (Camellia sinensis)

CAO Hong-Li,YUE Chuan,ZHOU Yan-Hua,WANG Lu,HAO Xin-Yuan,YANG Ya-Jun*,WANG Xin-Chao*   

  1. Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China?
  • Received:2014-01-08 Revised:2014-04-16 Online:2014-09-12 Published:2014-05-16
  • Contact: 王新超, E-mail: xcw75@tricaas.com, Tel: 0571-86653162; 杨亚军, E-mail: yjyang@tricaas.com, Tel: 0571-86650226

Abstract:

 

The basic leucine zipper proteins (bZIP) are one of the most extensive and conserved transcriptional factors families in eukaryotes, and plant bZIPs play important roles in many biological processes, especialy for resisting abiotic stresses. In this study, a bZIP full-length cDNA sequence was cloned using RACE and reverse transcription-PCR(RT-PCR) techniques from tea plant (Camellia sinensis). The obtained full-length cDNA was named CsbZIP1 with GenBank accession number JX050148.1. It is 1515 bp in length, containing a 813 bp open reading frame (ORF), encoding 270 amino acid residues with 29.484 kD molecular weight, and containing a typical BRLZ motif (basic region domain and leucine zipper domain) of B-zip1 family. The phylogenetic tree analysis revealed that CsbZIP1 belongs to F subfamily of bZIP. The subcellular location showed that CsbZIP1 protein is located in nucleus. The qRT-PCR analysis indicated that the expression level of CsbZIP1 was up-regulated by cold (4℃) and salt (NaCl) treatments, and both expression amounts increased at first, then declined after 24 hours. However, the expression pattern was down-regulated by ABA treatment within 24 hours. These results demonstrated that CsbZIP1 could be associated with cold and salt stresses in tea plant.

Key words: Tea plant (Camellia sinensis), bZIP transcription factor, CsbZIP1, Subcellular localization, Cloning and Expression

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