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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (05): 692-697.doi: 10.3724/SP.J.1006.2015.00692

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Evaluation of Selective Efficiency of Molecular Markers Linked with a Novel Gene Xa39 Resistant to Bacterial Blight in Rice

ZHUO Da-Long1,2,HU Dan-Dan1,2,ZHANG Fan2,ZHANG Fan2,*,SHI Ying-Yao1,*,GAO Yong-Ming2,ZHOU Yong-Li2,LI Zhi-Kang2   

  1. 1 College of Agronomy, Anhui Agricultural University, Hefei 230036, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-11-24 Revised:2015-03-19 Online:2015-05-12 Published:2015-03-30

Abstract:

 Xa39 is a novel and dominant resistance gene with broad spectrum for bacterial leaf blight in rice. In previous study, this gene was mapped on chromosome 11. In present study, FF329 carrying Xa39 gene as donor parent and BT4, BT6, BT12, BT18 as recipients were used to develop four F2 segregation populations (4FL10, 4FL14, 4FL17, 4FL21). Combining artificial inoculation with phenotype identification of bacterial leaf blight, the validity of three molecular markers tightly linked with Xa39, and two co-dominant molecular markers was investigated for marker-assisted selection (MAS). The results showed that the accuracy of marker RM26985 for MAS was 95.81% and that of marker RM206 was 93.61% in the four populations. The accuracy of two markers RM26985 and RM206 used simultaneously for MAS was up to 95.35%. Therefore, RM26985 and RM206 can be used as effective markers in MAS for Xa39 in rice breeding program.

Key words: Xa39, Rice bacterial blight, SSR, MAS

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