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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 70-81.

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Comparison of Starch Granule Morphology and Size Distribution in Superior and Inferior Grains of Three Cereal Crops

XU Yun-Ji,LI Yin-Yin,QIAN Xi-Yang,WANG Zhi-Qin,YANG Jian-Chang*   

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
  • Received:2015-05-31 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Contact: 杨建昌,E-mail: jcyang@yzu.edu.cn, Tel: 0514-87979317 E-mail:xuyunji19881004@163.com
  • Supported by:

    The research was supported by the National Natural Science Foundation of China (31271641, 31471438, 31461143015), China National Public Welfare Industry (Agriculture) Plan (201103003, 201203079), Jiangsu “Three-innovation” Agricultural Project (SXG2014313), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Abstract:

Using two rice cultivars, two wheat cultivars, and two maize cultivars, we extracted starch granules to observe the morphological characteristics and to compare the differences in the starch granule number, volume and surface area distributions among the three crops and between superior and inferior grains of each cultivar. The results showed that significant differences were observed in starch granule morphology and size among the three crops. The diameter of starch granule showed an order of maize > wheat > rice. Starch granules extracted in rice showed an irregular shape, the starch granules in wheat were lenticular-shaped and spherical-shaped. The starch granules in maize grain were mainly polyhedral or irregular and spherical in shape. Distributions of starch granule number, volume and surface area were changed in a typical unimodal-peak curve, a triple-peak curve and a typical bimodal-peak curve, respectively, in grains of rice and maize. Distributions of starch granule number, volume and surface area in wheat grains displayed a typical unimodal-peak curve, a four-peak curve and a triple-peak curve, respectively. According to starch granule diameters, all the starch granules were classified into small, medium, and large granules in this study. The thresholds for separating the starch granules were 1.5 μm and 20.0 μm, 5.0 μm and 50.0 μm, 4.0 μm and 50.0 μm, respectively, in rice, wheat and maize. The total volume of starch granules in grains of the three cereal crops was mainly determined by the volume of medium starch granules. No significant difference was observed in the proportions of small starch granule size and medium starch granule number between superior and inferior grains of the three cereal crops. But the volume and surface area of medium starch granules were larger in superior grains than in inferior ones. The percentage of large starch granules was greater in the inferior than in the superior. Changes in the volume of medium starch granules in both superior and inferior grains were consistent with those in starch accumulation and grain weight. The results suggest that the starch granule volume is an important factor determining grain weight. Increasing the volume of medium starch granules or reducing the volume of large starch granules would increase the weight of inferior grains.

Key words: Starch granule, Morphology, Size distribution, Superior grains, Inferior grains, Rice, Wheat, Maize

[1]Yang J, Cao Y, Zhang H, Liu L, Zhang J. Involvement of polyamines in the post-anthesis development of inferior and superior spikelets in rice. Planta, 2008, 228: 137–149

[2]Yang W B, Yin Y P, Li Y, Cai T, Ni Y L, Peng D L, Wang Z L. Interactions between polyamines and ethylene during grain filling in wheat grown under water deficit conditions. Plant Growth Regul, 2014, 72: 189–201

[3]徐云姬, 顾道健, 张博博, 张耗, 王志琴, 杨建昌. 玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系. 作物学报, 2013, 39: 1452–1461

Xu Y J, Gu D J, Zhang B B, Zhang H, Wang Z Q, Yang J C. Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize. Acta Agron Sin, 2013, 39: 1452–1461 (in Chinese with English abstract)

[4]Jiang D, Cao W X, Dai T B, Jing Q. Activities of key enzymes for starch synthesis in relation to growth of superior and inferior grains on winter wheat (Triticum aestivum L.) spike. Plant Growth Regul, 2003, 41: 247–257

[5]Li W, Yan S, Wang Z. Effect of spikelet position on starch proportion, granule distribution and related enzymes activity in wheat grain. Plant Soil Environ, 2013, 59: 568–574

[6]申丽霞, 王璞, 张红芳, 易镇邪. 施氮对夏玉米不同部位籽粒灌浆的影响. 作物学报, 2005, 31: 532–534

Shen L X, Wang P, Zhang H F, Yi Z X. Effect of nitrogen supply on grain filling at different ear position in summer maize. Acta Agron Sin, 2005, 31: 532–534 (in Chinese with English abstract)

[7]Paul C. The structure of starch. Nature, 1997, 389: 338–339

[8]Tester R F, Karkalas J, Qi X. Starch structure and digestibility enzyme-substrate relationship. World’s Poult Sci J, 2004, 60: 186−195

[9]Vermeylen R, Goderis B, Reynaers H, Delcour J A. Gelatinisation related structural aspects of small and large wheat starch granules. Carbohydr Polymers, 2005, 62: 170–181

[10]Kim H S, Huber K C. Channels within soft wheat starch A- and B-type granules. J Cereal Sci, 2008, 48: 159–172

[11]Peng M, Gao M, Abdel-Aal E S M, Hucl P, Chibbar R N. Separation and characterization of A- and B-type starch granules in wheat endosperm. Cereal Chem, 1999, 76: 375–379

[12]Bechtel D B, Zayas I, Kaleikau L, Pomeranz Y. Size-distribution of wheat starch granules during endosperm development. Cereal Chem, 1990, 67: 59−63

[13]张丽, 张吉旺, 刘鹏, 董树亭. 不同淀粉含量玉米籽粒淀粉粒度的分布特性. 中国农业科学, 2011, 44: 1596–1602

Zhang L, Zhang J W, Liu P, Dong S T. Starch granule size distribution in grains of maize with different starch contents. Sci Agric Sin, 2011, 44: 1596–1602 (in Chinese with English abstract)

[14]陆大雷, 郭换粉, 董策, 陆卫平. 普通、甜、糯玉米果穗不同部位籽粒淀粉理化特性和颗粒分布差异. 作物学报, 2011, 37: 331–338

Lu D L, Guo H F, Dong C, Lu W P. Starch physicochemical characteristics and granule size distribution at apical, medium and basal ear positions in normal, sweet, and waxy maize. Acta Agron Sin, 2011, 37: 331–338 (in Chinese with English abstract)

[15]蔡瑞国, 张敏, 朱桓, 武宝悦, 李彦生, 王振林. 糯小麦籽粒淀粉粒度分布特征. 麦类作物学报, 2010, 30: 254–258

    Cai R G, Zhang M, Zhu H, Wu B Y, Li Y S, Wang Z L. Starch granule size distribution in grains of waxy wheat. J Triticeae Crops, 2010, 30: 254–258 (in Chinese with English abstract)

[16]崔丽娜,董树亭. 2种玉米籽粒淀粉粒分布的比较. 中国粮油学报, 2013, 28(12): 29–32

    Cui L N, Dong S T. Comparison of grain starch granule distribution of two kinds of corn kernel(Zea mays L.). J Chin Cereals & Oils Assoc, 2013, 28(12): 29–32 (in Chinese with English abstract)

[17]戴忠民, 尹燕枰, 郑世英, 蔡瑞国, 顾锋, 闫素辉, 李文阳, 王振林. 不同供水条件对小麦强、弱势籽粒中淀粉粒度分布的影响. 生态学报, 2009, 29: 6534-6543

Dai Z M, Yin Y P, Zheng S Y, Cai R G, Gu F, Yan S H, Li W Y, Wang Z L. Effect of water regime on starch granule size distribution in superior and inferior grains of wheat. Acta Ecol Sin, 2009, 29: 6534–6543 (in Chinese with English abstract)

[18]崔丽娜, 董树亭. 氮肥对玉米子粒淀粉粒形态及分布的影响. 玉米科学, 2013, 21: 64–68

Cui L N, Dong S T. Effects of application nitrogen on maize(Zea mays L.) grain starch morphogenesis and size distribution. J Maize Sci, 2013, 21: 64–68 (in Chinese with English abstract)

[19]Peng D L, Cai T, Yin Y P, Yang W B, Ni Y L, Yang D Q, Wang Z L. Exogenous application of abscisic acid or gibberellin acid has different effects on starch granule size distribution in grains of wheat. J Integr Agric, 2013, 12: 1551–1559

[20]何照范. 粮油籽粒品质及其分析技术. 北京: 农业出版社, 1985

He Z F. Grain Quality and Its Analysis Technology. Beijing: Agriculture Press, 1985 (in Chinese)

[21]Peng M, Gao M, Abdel-Aal E S M, Hucl P, Chibbar R N. Separation and characterization of A- and B-type starch granules in wheat endosperm. Cereal Chem, 1999, 76: 375–379

[22]Ji Y, Wong K, Hasjim J, Pollak L M, Duvick S, Jane J, White P J. Structure and function of starch from advanced generations of new corn lines. Carbohydr Polymers, 2003, 54: 305–319

[23]Shapter F M, Henry R J, Lee L S. Endosperm and starch granule morphology in wild cereal relatives. Plant Genet Resour Characterization and Utilization, 2008, 6: 85–97

[24]Wei C X, Qin F L, Zhu L J, Zhou W D, Chen Y F, Wang Y P, Gu M H, Liu Q Q. Microstructure and ultra-structure of high-amylose rice resistant starch granules modified by antisense RNA inhibition of starch branching enzyme. J Agric Food Chem, 2010, 58: 1224–1232

[25]Yu X R, Zhou L, Zhang J, Yu H, Xiong F, Wang Z. Comparison of starch granule development and physicochemical properties of starches in wheat pericarp and endosperm. J Sci Food Agric, 2015, 95: 148–157

[26]Cai C H, Lin L S, Man J M, Zhao L X, Wang Z F, Wei C X. Different structural properties of high-amylose maize starch fractions varying in granule size. J Agric Food Chem, 2014, 62: 11711–11721

[27]Dhital S, Butardo V M, Jobling S A, Gidley M J. Rice starch granule amylolysis-differentiating effects of particle size, morphology, thermal properties and crystalline polymorph. Carbohydr Polymers, 2015, 115: 305–316

[28]戴忠民, 王振林, 张敏, 李文阳, 闫素辉, 蔡瑞国, 尹燕枰. 不同品质类型小麦籽粒淀粉粒度的分布特征. 作物学报, 2008, 34: 465–470

    Dai Z M, Wang Z L, Zhang M, Li W Y, Yan S H, Cai R G, Yin Y P. Starch granule size distribution in grains of strong and weak gluten wheat cultivars. Acta Agron Sin, 2008, 34: 465–470 (in Chinese with English abstract)

[29]蔡瑞国, 尹燕枰, 赵发茂, 张敏, 张体彬, 梁太波, 顾锋, 戴忠民, 王振林. 强筋小麦胚乳淀粉粒度分布特征及其对弱光的响应. 中国农业科学, 2008, 41: 1308–1316

    Cai R G, Yin Y P, Zhao F M, Zhang M, Zhang T B, Liang T B, Gu F, Dai Z M, Wang Z L. Size distribution of starch granules in strong-gluten wheat endosperm under low light environment. Sci Agric Sin, 2008, 41: 1308–1316 (in Chinese with English abstract)

[30]余静, 冉从福, 李学军, 邵慧, 李立群. 陕糯1号与非糯小麦西农1330胚乳发育及淀粉形态、粒径分析. 中国农业科学, 2014, 47: 4405–4416

    Yu J, Ran C F, Li X J, Shao H, Li L Q. Study on endosperm development and morphological features of starch granules in waxy wheat Shannuo 1 and non-waxy wheat Xinong 1330. Sci Agric Sin, 2014, 47: 4405–4416 (in Chinese with English abstract)

[31]石德杨, 张海艳, 董树亭. 补充灌溉和施氮对玉米籽粒淀粉粒粒度分布的影响. 中国农业科学, 2014, 47: 633–643

    Shi D Y, Zhang H Y, Dong S T. Effects of supplemental irrigation and nitrogen application on starch granule size distribution of maize grain. Sci Agric Sin, 2014, 47: 633–643 (in Chinese with English abstract)

[32]崔丽娜, 张红, 孟佳佳, 石德杨, 董树亭. 不同胚乳类型玉米籽粒淀粉粒的粒度分布特征. 作物学报, 2012, 38: 1723–1727

Cui L N, Zhang H, Meng J J, Shi D Y, Dong S T. Starch granule size distribution in maize kernel with different endosperm types. Acta Agron Sin, 2012, 38: 1723–1727 (in Chinese with English abstract)

[33]Zhang C H, Jiang D, Liu F L, Cai J, Dai T B, Cao W X. Starch granules size distribution in superior and inferior grains of wheat is related to enzyme activities and their gene expressions during grain filling. J Cereal Sci, 2010, 51: 226–233

[34]张蕊, 丁艳锋, 李刚华, 王强盛, 刘正辉, 王绍华. 水稻强弱势粒间淀粉粒和蛋白体积累的差异. 南京农业大学学报, 2014, 37(1): 15–20

Zhang R, Ding Y F, Li G H, Wang Q S, Liu Z H, Wang S H. Variation of starch granule and protein bodies in endosperm from superior and inferior grains of japonica rice. J Nanjing Agric Univ, 2014, 37(1): 15–20 (in Chinese with English abstract)

[35]谭秀山, 毕建杰, 王金花, 叶宝兴. 冬小麦不同穗位籽粒淀粉粒差异及其与粒重的相关性. 作物学报, 2012, 38: 1920–1929

   Tan X S, Bi J J, Wang J H, Ye B X. Differences of starch granules in grains from different spikelet positions and their correlation with grain weight in winter wheat. Acta Agron Sin, 2012, 38: 1920–1929 (in Chinese with English abstract)

[36]Peng M, Gao M, Baga M, Hucl P, Chibbar R N. Starch-branching enzymes preferentially associated with A-type starch granules in wheat endosperm. Plant Physiol, 2000, 124: 265–272

[37]伊祖涛, 张海艳. 糯玉米胚乳淀粉粒粒度分布形成的酶学机理. 植物生理学报, 2015, 51: 88–92

Yi Z T, Zhang H Y. Enzyme mechanism of starch granule size distribution formation in waxy corn endosperm. Acta Phytophysiol Sin, 2015, 51: 88–92 (in Chinese with English abstract)

[38]McMaugh S J, Thistleton J L, Anschaw E, Luo J X, Konik-Rose C, Wang H, Huang M, Larroque O, Regina A, Jobling S A, Morell M K, Li Z Y. Suppression of starch synthase I expression affects the granule morphology and granule size and fine structure of starch in wheat endosperm. J Exp Bot, 2014, 65: 2189–2201

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