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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 532-539.doi: 10.3724/SP.J.1006.2016.00532

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

DNA Methylation Dynamic Analysis of Self Compatible Line and Self-Incompatible Line of Brassica oleracea var. acephala at Seed Germination Stage

ZHANG Yang1,HU Zhong-Ying1,ZHAO Yue-Ming1,LI Na2,XIE Li-Nan1,*   

  1. 1 College of Life Sciences, Northeast Forestry University, Harbin 150040, China; 2 Heilongjiang Vocational College of Biology and Technology, Harbin 150025, China
  • Received:2015-07-21 Revised:2015-11-20 Online:2016-04-12 Published:2015-12-18
  • Contact: 解莉楠, E-mail: linanxie@126.com E-mail:summerzhang@126.com
  • Supported by:

    This study weresupported by the Basic Scientific Research Expensesof the Higher Education Institutions of Central Government, China(DL09AB13) and the Subject of Innovative Talents for Science and Technology research in Harbin(Youth talents in science and technology innovation) (2013RFQXJ036).

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Abstract:

The seed of self-incompatible line often degraded. This studyaims to clarify the relationship between the degradation of the seeds and methylation. In this experiment, methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9# and self-compatible line 14#.The improved CTAB method is adopted to extract seed germination in different periods of DNA, and then through the MSAP analysis, statistical amplification bands, compare the differences between 9# and 14#. The results are shown as follows: DNA methylation modification throughout the whole process of seed germination of 9#, at the early stage of the germination (0 to 2 days) methylation sites continued to increase; at the later stage (2 to 8 days) demethylation increased apparently, and eventually the number of demethylation was 11 times more than methylation. It was proved that DNA methylation modification was an important way to regulate the gene expression during seed germination of self-incompatible line 9#. Self-incompatible line 9# and self-compatible line 14# had different DNA methylation status clearly at the respectively periods of 0 and 2 days after seed germination.The proportion of total methylation, full-methylation and semi-methylation of 9# was all higher than of 14# at the same period. As seedings continued to grow after germination, in 9#, the proportion of full-methylation increased clearly and almost that of semi-methylation did not change, while in 14#, the proportion of semi-methylation increased clearly and almost that of full-methylation did not change.

Key words: Brassica oleracea var. acephala, Self-incompatibility, Self-compatibility, DNA methylation

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