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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 405-413.doi: 10.3724/SP.J.1006.2015.00405

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloningand Expression Pattern of DNA Methylase I (MET1) from Brassica napus L. and Its Progenitors

TAN He-Lin1,*,XU Xin-Ying1,FU Li-Man1,XIANG Xiao-E2,LI Jian-Qiao1,GUO Hao-Lun1,YE Wen-Xue1   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China;2Animal Sciences National Teaching Demonstration Center,Nanjing Agricultural University, Nanjing 210095, China
  • Received:2014-09-03 Revised:2014-12-19 Online:2015-03-12 Published:2015-01-12

Abstract:

Methylation in genomic DNA, a way of epigenetic regulation, plays animportant role during the growth and development of plant. As allotetraploid oilseeds plant, Brassica napus (AACC, 2n=38) originated from the natural cross between Brassica rapa (AA, 2n=20) and Brassica oleracea (CC, 2n=18), containing duplicate genes. Here, we isolated and characterized five Brassica napus BnMET1s, which are orthologous gene of Arabidopsis AtMET1 acted as transferase in DNA methylation. Our results showed that there were conspicuous variations in gene structuresof BnMET1s compared with its orthologos of BrMET1 and BoMET1, leading to more abundant divergences in coding region of BnMET1s. Moreover, we found that some divergences among BnMET1paralogous genes were derived from its progenitor orthologousgenes of Brassica rapa or Brassica oleracea. Furthermore, the transcription analysis indicated that partialBnMET1paralogs were silence,and the expression patternsof the activated BnMET1were altered in contrast to its BoMET1 orthologs in Brassica oleracea and BrMET1 orthologs in Brassica rapa. Taken all these together, we speculated that duplicate BnMET1s regulate the development process ofBrassica napuswitha certain gene dosagekept by alteringtheir gene structures and spatio-temporal expression patterns.

Key words: DNA methylation, MET1 geneGene evolution, Differential expression

[1]Finnegan E J, Genger R K, Peacock W J, Dennis E S. DNA methylation in plants. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 223–247

[2]Saze H, Sasaki T, Kakutani T. Negative regulation of DNA methylation in plants. Epigenetics, 2008, 3: 122–124

[3]Teixeira F K, Colot V. Gene body DNA methylation in plants: a means to an end or an end to a means? EMBO J, 2009, 28: 997–998

[4]Finnegan E J, Kovac K A, Jaligot E, Sheldon C C, Peacock W J, Dennis E S. The downregulation of FLOWERING LOCUS C (FLC) expression in plants with low levels of DNA methylation and by vernalization occurs by distinct mechanisms. Plant J, 2005, 44: 420–432

[5]Vanyushin B F, Kirnos M D. DNA methylation in higher plants: past, present and future. Biochim Biophys Acta, 2011, 1809: 360–368

[6]Vanyushin B F. DNA methylation in plants. Curr Top Micro-biol Immunol, 2006, 301: 67–122

[7]Meyer P. DNA methylation systems and targets in plants. FEBS Lett, 2011, 585: 2008–2015

[8]Law J A, Jacobsen S E. Establishing, maintaining and modi-fying DNA methylation patterns in plants and animals. Nat Rev Genet, 2010, 11: 204–220

[9]夏晗, 刘美芹, 尹伟伦, 卢存福, 夏新莉. 植物DNA甲基化调控因子研究进展. 遗传, 2008, 30: 426–432

Xia H, Liu M Q, Yi W L, Lu C F, Xia X L. DNA methylation regulating factors in plants. Hereditas (Beijing), 2008, 30: 426–432 (in Chinese with English abstract)

[10]Finnegan E J, Peacock W J, Dennis E S. Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci USA, 1996, 93: 8449–8454

[11]Apashkin V V, Kutueva L I, Vaniushin B F. Is the cytosine DNA methyltransferase gene MET1 regulated by DNA methylation in Arabidopsis thaliana plants? Genetika, 2011, 47: 320–331

[12]Kankel M W, Ramsey D E, Stokes T L, Flowers S K, Haag J R, Jeddeloh J A, Riddle N C, Verbsky M L, Richards E J. Arabidopsis MET1 cytosine methyltransferase mutants. Genetics, 2003, 163: 1109–1122

[13]Soppe W J, Jacobsen S E, Alonso-Blanco C, Jackson J P, Kakutani T, Koornneef M, Peeters A J. The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene. Mol Cell, 2000, 6: 791–802

[14]闫延涛. 普通小麦中春化基因VRN1及甲基转移酶基因的克隆及表达. 河南农业大学硕士学位论文, 河南郑州 2010

Yan Y T. Cloning and expression of the vernalization gene VRN1 and DNA methyltransferase in Wheat. MS Thesis of Henan              Agricultural University, Zhengzhou, China, 2010 (in Chinese with English abstract)

[15]Nakano Y, Steward N, Sekine M, Kusano T, Sano H. A tobacco NtMET1 cDNA encoding a DNA methyltransferase: molecular characterization and abnormal phenotype of transgene tobacco plants. Plant Cell Physiol, 2000, 41: 448–457

[16]Chang L, Zhang Z, Han B, Li H, Dai H, He P, Tian H. Isolation of DNA methyltransferase genes from strawberry (Fragaria × ananassa Duch.) and their expression in relation to micropropagation. Plant Cell Rep, 2009, 28: 1373–1384

[17]Steward N, Kusano T, Sano H. Expression of ZmMET1, a gene encoding a DNA methyltransferase from maize, is associated not only with DNA replication in actively proliferating cells but also with altered DNA methylation status in cold-stressed quiescent cells. Nucl Acids Res, 2000, 28: 3250–3259

[18]Zhang T, Zhao X, Wang W, Pan Y, Huang L, Liu X, Zong Y, Zhu L, Yang D, Fu B. Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes. PLoS One, 2012, 7: e43274



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