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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 551-560.doi: 10.3724/SP.J.1006.2016.00551

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of the Mutant with Low Chlorophyll Content onPhotosynthesis and Yield in Rice

GU Jun-Fei*,ZHOU Zhen-Xiang,LI Zhi-Kang,DAI Qi-Xing,KONG Xiang-Sheng,WANG Zhi-Qin,YANG Jian-Chang   

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
  • Received:2015-08-19 Revised:2016-01-11 Online:2016-04-12 Published:2016-01-25
  • Contact: 顾骏飞, E-mail: gujf@yzu.edu.cn, Tel: 0514-87979381 E-mail:gujf@yzu.edu.cn
  • Supported by:

    This study was supported by the National Basic Research Program of China (973, 2015CB150401), National Natural Science Foundation of China (31501254, 31371562), the Natural Science Foundation of Jiangsu Province (BK20140480),China Postdoctoral Science Foundation (2014M550312, 2015T80590), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (14KJB210007),and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Abstract:

A chlorophyll-deficit rice mutant (YL) with ~51% chlorophyll of its wild type (WT) was 3.7%, 20.4%, and 39.1% higher in photosynthesisthan WT under saturating light condition,in treatments of0 kg N ha-1, 120 kg N ha-1, 240 kg N ha-1, respectively. In the field and pot experiments, we studied leaf Rubisco content, stomatal conductance, expression levels of aquaporin genes, chlorophyll fluorescence, light and electron micrographs at different levels of N application. The results showed that the decreased level of chlorophyll content in YL was compensated by a relativelyhigher quantum yield of PSII. The electron micrographs of chloroplasts showed that there were no differences in chloroplast development between YL and WT. The stomatal conductance was much higher in the mutant than in wild type, and expression levels of the aquaporin genes suggested a higher mesophyll conductance in YL. The higherCO2 conductance together with a higher Rubisco content in YL could be reasons for the higher photosynthetic rate. The yield of YL was similar to that of WT, but the growth duration in YL was much shorter, which could be caused by the different photosynthetic performance between YL and WT. All these results implicate that higher photosynthetic rate doesnot necessitate higher chlorophyll content. Moderate chlorophyll content will benefit the leaf photosynthesis. Decreasing N investment in chlorophyll synthesis and optimizing N distribution among different photosynthetic compounds could potentially improve photosynthesis and yield. The YL material used in this study could be potentially used to improve photosynthetic efficiency in breeding programmes.

Key words: Rice, Chlorophyll, Photosynthesis, High photosynthetic efficiency, Nitrogen

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