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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1161-1169.doi: 10.3724/SP.J.1006.2017.01161

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Soybean Transcription Factor Gene GmNF-YCa Enhances Osmotic Stress Tolerance of Transgenic Arabidopsis

LI Min 1,2,YU Tai-Fei1,2,XU Zhao-Shi2,ZHANG Shuang-Xi 3,MIN Dong-Hong 1,CHEN Ming2,MA You-Zhi2,CHAI Shou-Cheng 1,*,ZHENG Wei-Jun 1,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China; 3 Institute of Crop Science, Ningxia Academy of Agriculture and Forestry Sciences, Yongning 750105, China
  • Received:2016-12-20 Revised:2017-04-20 Online:2017-08-12 Published:2017-05-11
  • Contact: zheng Weijun, E-mail: zhengweijun@nwsuaf.edu.cn; Cai Shoucheng, E-mail: chaishoucheng@126.com E-mail:13664207046@163.com
  • Supported by:

    This study was supported by the National Major Project for Developing New GM Crops (2014ZX0800916B) and the National Natural Science Foundation of China (31371620).

Abstract:

Biotic stresses, like plant diseases and insect pests, and abiotic stresses, such as drought and salt, heavily threaten plant growth and influence crop yield and quality. Osmotic stress resulting from water deficiency in the soil is one of major hurdles. Nuclear Factor Y (NF-Y) is a heterotrimeric protein, consisting of NF-YA, NF-YB and NF-YC in plant. NF-Y plays significant roles in the pathway responding to osmotic stresses in plants. We acquired a gene GmNF-YCa, a member of regulatory subunit NF-YC family in soybean (Glycine max L.), by screening a soybean cDNA library using yeast two-hybrid system. The full sequence of GmNF-YCa is 864 bp, encoding 287 amino acids and having a NF-YC motif, belonging to NF-YC subfamily. GmNF-YCa is a hydrophilic protein with a molecular weight of 31.6 kD and isoelectric point of 5.07, and containing a transmembrane domain and no any signal peptides. Sequence analysis showed that NF-YC subfamily was highly conserved among various species. Promoters of GmNF-YCa contained various abiotic stresses and light responsive elements, such as ARE, Box 4, GATA-motif, Box I, ACE, ABRE,CAT-Box. According to tissue-specific analysis, GmNF-YCa had the highest expression level in germination stage. Quantitative Real-time PCR suggested that GmNF-YCa was induced by sucrose stress and mannitol treatment. GmNF-YCa was transformated to Arabidopsis successfully by Agrobacterim-mediated method and the overexpressed Arabidopsis was prepared for the function characterization analysis. Overexpression of GmNF-YCacould improve tolerance of transgenic Arabidopsis to osmotic stress in the germination stage, and enhance root development with more lateral roots in sucrose and mannitol treatments.

Key words: Soybean, Nuclear Factor Y, Expression pattern, Promoter, Osmotic stress

[1]  Xu Z S, Chen M, Li L C, Ma Y Z. Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol, 2011, 53: 570–585
[2]  Xu Z S, Chen M, Li L C, Ma Y Z. Functions of the ERF transcription factor family in plants. Botany, 2008, 86: 969–977
[3]  Mahajan S, Tuteja N. Cold, salinity and drought stresses: an overview. Arch Biochem Biophys, 2005, 444: 139–158
[4]  Mantovani R. The molecular biology of the CCAAT-binding factor NF-Y. Gene, 1999, 239: 15–27
[5]  Xing Y, Fikes D J, Guarente L. Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box-binding domain. EMBO J, 1993, 12: 4647–4655.
[6]  Romier C, Romier C, Cocchiarella F, Mantovani R, Moras D. The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y. J Biol Chem, 2003, 278: 1336–1345
[7]  Coustry F, Maity S N, de Crombrugghe B. The transcriptional activity of the CCAAT binding factor CBF is mediated by two distinct activation domains, one in the CBF-B subunit and the other in the CBF-C subunit. J Biol Chem, 1996, 271: 14485–14491
[8]  Hu Q, Lu J F, Luo R, Sen S . Inhibition of CBF/NF-Y mediated transcription activation arrests cells at G(2)/M phase and suppresses expression of genes activated at G(2)/M phase of the cell cycle. Nucl Acids Res, 2006, 34: 6272–6285
[9]  Korner K, Jérôme V, Schmidt T, Müller R. Cell cycle regulation of the murine cdc25B promoter–essential role for nuclear factor-Y and a proximal repressor element. J Biol Chem, 2001, 276: 9662–9669
[10] Gurtner A, Fuschi P, Magi F, Colussi C. NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue. PLoS One, 2008, 3: e2407
[11] Bhattacharya A, Deng J M, Zhang Z, Behringer R. The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation. Cancer Res, 2003, 63: 8167–8172
[12] Edwards D, Murray J A H, Smith A.G.. Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis. Plant Physiol, 1998, 117: 1015–1022
[13] Stephenson T J, McIntyre C L, Collet C, Xue G P. Genome-wide identification and expression analysis of the NF-Y family of transcription factors in Triticum aestivum. Plant Mol Biol, 2007, 65: 77–92
[14] Lawton-Rauh A. Evolutionary dynamics of duplicated genes in plants. Mol Phylogenet Evol, 2003, 29: 396–409
[15] Maere S, De Bodt S, Raes J, Casneuf T, Van Montagu M, Kuiper M, Van de PeerY. Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci USA, 2005, 102: 5454–5459
[16] Laloum T, Mita De S, Gamas P, Baudin M. CCAAT-box binding transcription factors in plants: Y so many? Trends Plant Sci, 2013, 18: 157–166
[17] Kumimoto R W, Adam L, Hymus G J, Repetti P P, Reuber T L. The nuclear factor Y subunits NF-YB2 and NF-YB3 play additive roles in the promotion of flowering by inductive long-day photoperiods in Arabidopsis. Planta, 2008, 228: 709–723
[18] Yamamoto A, Kagaya Y, Toyoshima R, Kagaya M, Takeda S. Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors. Plant J, 2009, 58: 843–856
 [19] Nelson D E, Repetti P P, Adams T R, Creelman R A, Wu J. Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA, 2007, 104: 16450–16455
[20] Feng Z J, Cui X Y, Chen M, Yang G X, Ma Y Z, He G Y, Xu Z S. The soybean GmDi19-5 interacts with GmLEA3.1 and increases sensitivity of transgenic plants to abiotic stresses. Front Plant Sci, 2015, 6: 179
[21] Wei M L, Pei L L, Liu J M, Min D H. Isolation and Molecular Characteristics Analysis of Soybean Transcription Factor Gene GmMYB174. J Plant Genet Resour, 2015, 16 : 94–99
[22] Beehtold N, Ellis J, Pelletier G. In plant Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. Life Sci, 1993, 316: 1194–1199
[23] Kusnetsov V, Landsberger M, Meurer J, Oelmüller R. The assembly of the CAAT-box binding complex at a photosynthesis gene promoter is regulated by light, cytokinin, and the stage of the plastids. J Biol Chem, 1999, 274: 36009–36014
[24] Kumimoto R W, Zhang Y, Siefers N. BFH Iii. NF-YC3, NF-YC4 and NF-YC9 are required for CONSTANS-mediated, photoperiod-dependent flowering in Arabidopsis thaliana. Plant J, 2010, 63: 379–391
[25] Yu Y L, Li Y Z, Huang G X, Meng Z D, Zhang D, Wei J, Yan K, Zheng C C, Zhang L Y. PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii. J Exp Bot, 2011, 62: 4805–4817
[26] Stephenson T J, McIntyre L C, Collet C, Xue G P. TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes. Funct Integr Genom, 2010, 10: 265–276
[27] Ni, Z, Hu Z, Jiang Q, Zhang H. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress. Plant Mol Biol, 2013, 82: 113–129
[28] Han X, Tang S, An Y, Zheng D C. Overexpression of the poplar NF-YB7 transcription factor confers drought tolerance, improves water-use efficiency in Arabidopsis. J Exp Bot, 2013, 64: 4589–4601
[29] Li W X, Oono Y, Zhu J, He X J, Wu J M. The Arabidopsis NFYA5 transcription factor is regulated transcriptionally, post transcriptionally to promote drought resistance. Plant Cell, 2008, 2: 2238–2251
 
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