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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1161-1169.doi: 10.3724/SP.J.1006.2017.01161

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Soybean Transcription Factor Gene GmNF-YCa Enhances Osmotic Stress Tolerance of Transgenic Arabidopsis

LI Min 1,2,YU Tai-Fei1,2,XU Zhao-Shi2,ZHANG Shuang-Xi 3,MIN Dong-Hong 1,CHEN Ming2,MA You-Zhi2,CHAI Shou-Cheng 1,*,ZHENG Wei-Jun 1,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China; 3 Institute of Crop Science, Ningxia Academy of Agriculture and Forestry Sciences, Yongning 750105, China
  • Received:2016-12-20 Revised:2017-04-20 Online:2017-08-12 Published:2017-05-11
  • Contact: zheng Weijun, E-mail: zhengweijun@nwsuaf.edu.cn; Cai Shoucheng, E-mail: chaishoucheng@126.com E-mail:13664207046@163.com
  • Supported by:

    This study was supported by the National Major Project for Developing New GM Crops (2014ZX0800916B) and the National Natural Science Foundation of China (31371620).

Abstract:

Biotic stresses, like plant diseases and insect pests, and abiotic stresses, such as drought and salt, heavily threaten plant growth and influence crop yield and quality. Osmotic stress resulting from water deficiency in the soil is one of major hurdles. Nuclear Factor Y (NF-Y) is a heterotrimeric protein, consisting of NF-YA, NF-YB and NF-YC in plant. NF-Y plays significant roles in the pathway responding to osmotic stresses in plants. We acquired a gene GmNF-YCa, a member of regulatory subunit NF-YC family in soybean (Glycine max L.), by screening a soybean cDNA library using yeast two-hybrid system. The full sequence of GmNF-YCa is 864 bp, encoding 287 amino acids and having a NF-YC motif, belonging to NF-YC subfamily. GmNF-YCa is a hydrophilic protein with a molecular weight of 31.6 kD and isoelectric point of 5.07, and containing a transmembrane domain and no any signal peptides. Sequence analysis showed that NF-YC subfamily was highly conserved among various species. Promoters of GmNF-YCa contained various abiotic stresses and light responsive elements, such as ARE, Box 4, GATA-motif, Box I, ACE, ABRE,CAT-Box. According to tissue-specific analysis, GmNF-YCa had the highest expression level in germination stage. Quantitative Real-time PCR suggested that GmNF-YCa was induced by sucrose stress and mannitol treatment. GmNF-YCa was transformated to Arabidopsis successfully by Agrobacterim-mediated method and the overexpressed Arabidopsis was prepared for the function characterization analysis. Overexpression of GmNF-YCacould improve tolerance of transgenic Arabidopsis to osmotic stress in the germination stage, and enhance root development with more lateral roots in sucrose and mannitol treatments.

Key words: Soybean, Nuclear Factor Y, Expression pattern, Promoter, Osmotic stress

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