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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (04): 505-511.doi: 10.3724/SP.J.1006.2018.00505

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with Functional Marker Selection of Fhb1 Gene

Hong-Jun ZHANG1(), Zhen-Qi SU2,3, Gui-Hua BAI3,4, Xu ZHANG5, Hong-Xiang MA5, Teng LI1, Yun DENG6, Chun-Yan MAI7, Li-Qiang YU8, Hong-Wei LIU1, Li YANG1, Hong-Jie LI1,*(), Yang ZHOU1,*()   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China
    3 Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA
    4 USDA-ARS / Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506, USA
    5 Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Key Laboratory of Agrobiology, Nanjing 210014, Jiangsu, China
    6 Nanping Institute of Agricultural Sciences of Fujian Province, Jianyang 354200, Fujian, China
    7 Xinxiang Innovation Center for Breeding Technology of Dwarf-Male-Sterile Wheat, Xinxiang 453731, Henan, China
    8 Zhaoxian Experiment Station, Shijiazhuang Academy of Agriculture and Forestry Sciences, Zhaoxian 515300, Hebei, China
  • Received:2017-07-19 Accepted:2018-01-08 Online:2018-01-29 Published:2018-01-29
  • Contact: Hong-Jie LI,Yang ZHOU E-mail:zhanghongjun01@caas.cn;lihongjie@caas.cn;zhouyang@caas.cn
  • Supported by:
    This study was supported by the National Key Research and Development Program of China (2016YFD0101802, 2017YFD0100600) and the National Engineering Laboratory of Crop Molecular Breeding.

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Abstract:

Fusarium head blight (FHB) becomes a major disease in the wheat production of the Yellow-Huai Rivers Valley Winter Wheat Zone of China. Improving FHB resistance is one of the major breeding targets in this region. The donors of Fhb1 gene, including Ningmai 9, Shengxuan 6, Jianyang 798, Jianyang 84, Sumai 3, and Ningmai 13, were moderately or highly resistant to FHB. Six backcrossing populations were developed by crossing these donors with a dwarfing and male-sterile wheat near isogenic line Zhoumai 16, and backcrossing with Zhoumai 16. The progenies were subjected to phenotypic evaluation of FHB by both the floret-inoculation method and natural infection in a field disease nursery. Fhb1 was detected by a functional Kompetitive Allele Specific PCR (KASP) marker. The backcrossing progenies with Fhb1 gene were moderately susceptible to FHB. Compared with the backcrossing progenies without Fhb1 gene, mean number of diseased spikelets and disease index reduced by 4.2 (P < 0.01) and 4.0 in the progenies with Fhb1 gene, respectively. The mean number of diseased spikelets and disease index for the backcrossing progenies with Fhb1 gene were 8.1 (P < 0.01) and 28.4 (P < 0.01) lower than those of the recurrent parent Zhoumai 16. There were significant differences in resistance among the backcrossing progenies from different donors. The progenies from Shengxuan 6 had better performance in number of diseased spikelets and disease index compared with those from any other donors. The results from this study indicate that Fhb1 gene can efficiently improve the FHB resistance of wheat cultivars grown in the Yellow-Huai Rivers Valley Winter Wheat Zone.

Key words: Triticum aestivum L., Fusarium head blight resistance, Fhb1 gene, molecular marker-assisted selection

Fig. 1

Performance of FHB resistance in the backcrossing progenies with or without Fhb1 gene from different donors, recurrent parent and controls using the floret-inoculation method The number of diseased spikelets of backcrossing progenies with Fhb1 gene from donors Ningmai 9 (NM 9, A), Shengxuan 6 (SX 6, B), Jianyang 798 (JY 798, C), Jianyang 84 (JY 84, D), Sumai 3 (SM 3, E), and Ningmai 13 (NM 13, F) were obviously lower than the backcrossing progenies without Fhb1 gene. The recurrent parent Zhoumai 16 (ZM 16) and the highly susceptible control Annong 8455 (AN 8455) had more number of diseased spikelets than Huaimai 20 (HM 20), the moderately susceptible control (G) and Yangmai 158 (YM 158), the moderately resistant control (H). +: Fhb1 genotype; –: non-Fhb1 genotype; RP: recurrent parent; MS: moderately susceptible; HS: highly susceptible; MR: moderately resistant."

Fig. 2

Comparison of number of diseased spikelets and disease index between backcrossing progenies with Fhb1 gene from different donors and recurrent parent, moderately susceptible control and the backcrossing progenies without Fhb1 gene Comparisons of the number of diseased spikelets and disease index were carried out between all Fhb1 backcrossing progenies and the recurrent parent Zhoumai 16 (A), the moderately susceptible control Huaimai 20 (B) and all non-Fhb1 backcrossing progenies (C), and between non-Fhb1 and Fhb1 backcrossing progenies from the donor cultivars Ningmai 9 (D), Shengxuan 6 (E), Jianyang 798 (F), Jianyang 84 (G), Sumai 3 (H) and Ningmai 13 (I). NDS: number of diseased spikelets (floret-inoculation method); DI: disease index (natural infection nursery); ++: all Fhb1 backcrossing progenies; RP: recurrent parent; MS: moderately susceptible control; ––: all Fhb1 backcrossing progenies; +: Fhb1 backcrossing progenies; –: non-Fhb1 backcrossing progenies. * and ** above the error bars indicate significant difference at P < 0.05 and P < 0.01, respectively."

Table 1

Comparison of number of diseased spikelets and disease index among the backcrossing progenies with Fhb1 gene from different donors"

品种(系)或回交后代
Cultivars (lines) or backcrossing progenies		 病小穗数 No. of diseased spikelets 病情指数Disease index
平均值±标准差 Mean±SD 范围
Range		 平均值±标准差 Mean±SD 范围
Range
宁麦9号回交后代 Ningmai 9 backcrossing progenies 6.5±3.4 de 1.0-13.0 70.9±6.1 ab 61.8-78.8
生选6号回交后代 Shengxuan 6 backcrossing progenies 3.9±3.2 f 1.0-10.0 56.4±7.5 c 48.8-63.8
建阳798回交后代 Jianyang 798 backcrossing progenies 9.5±4.8 c 1.0-20.0 65.6±10.9 c 52.4-76.4
建阳84回交后代 Jianyang 84 backcrossing progenies 5.6±3.9 e 1.0-12.0 65.9±19.7 ab 34.4-86.1
苏麦3号回交后代 Sumai 3 backcrossing progenies 4.8±4.1 ef 1.0-13.0 70.8±12.3 ab 48.1-84.7
宁麦13回交后代 Ningmai 13 backcrossing progenies 7.4±5.6 d 1.0-20.0 81.5±16.4 ab 63.2-95.0
轮回亲本-周麦16 Recurrent parent-Zhoumai 16 14.4±6.0 b 7.0-20.0 96.1±1.4 a 95.0-97.6
中感对照-淮麦20 MS control-Huaimai 20 6.2±2.2 de 2.0-12.0 68.6±11.3 ab 56.9-81.0
中抗对照-扬麦158 MR control-Yangmai 158 3.2±2.2 f 1.0-10.0 — —
高感对照-安农8455 HS control-Annong 8455 19.7±1.4 a 12.0-20.0 — —
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