油菜抗根肿病资源创新与利用的研究进展与展望

doi:10.3724/SP.J.1006.2018.01592

Abstract

Abstract:

Clubroot is one of the most serious soil-borne diseases caused by Plasmodiophora brassicae Woronin in oilseed rape ( Brassica napus). Being a major oil crop, the safe production for oilseed rape both in Canada and China is now facing severe threat from clubroot disease in recent years. In this review, after briefly introducing the biological features of this pathogen, the root symptoms after infection, pathogen-type differentiation, strategies usually employed in disease controlling process, and a strategy based on genetic breeding are highlighted in clubroot disease comprehensive control for oilseed rape; furthermore, for sustainable development of oilseed rape industry by avoiding damages caused by clubroot disease, major problems faced are pointed out and some prospects are addressed accordingly.

Key words: Brassica napus, clubroot disease, germplasm innovation, resistant gene mapping, resistant breeding, progress and prospect

Ying-Fen JIANG,Zong-Xiang ZHAN,Zhong-Yun PIAO,Chun-Yu ZHANG. Progresses and Prospects of Germplasms Innovation for Clubroot Resistance and Genetic Improvement in Brassica napus[J].Acta Agronomica Sinica, 2018, 44(11): 1592-1599.

References 76

[1]	Dixon G R . The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. J Plant Growth Reg, 2009,28:194-202
[2]	Kageyama K, Asano T, Dixon G R . Life cycle of Plasmodio phora brassicae. J Plant Growth Regul, 2009,28:203-211
[3]	王靖, 黄云, 李小兰, 黎怀忠 . 十字花科根肿病研究进展. 植物保护, 2011,37(6):153-158 doi: 10.3969/j.issn.0529-1542.2011.06.031
	Wang J, Huang Y, Li X L, Li H Z . Research progress in clubroot of crucifers. Plant Prot, 2011,37(6):153-158 (in Chinese with English abstract) doi: 10.3969/j.issn.0529-1542.2011.06.031
[4]	Hwang S F, Strelkov S E, Feng J, Gossen B D, Howard R J . Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola( Brassica napus) crop. Mol Plant Pathol, 2012,13:105-113
[5]	Neuhauser S, Kirchmair M, Gleason F H . The ecological potentials of Phytomyxea (‘ plasmodiophorids’) in aquatic food webs. Hydrobiologia, 2011,659:23-25
[6]	Buczacki S T, Clay C M . Some observations on secondary zoospore development in Plasmodiophora brassicae. Trans Br Mycol Soc, 1984,82:339-342
[7]	Feng J, Xiao Q, Hwang S F, Strelkov S E, Gossen B D . Infection of canola by secondary zoospores of Plasmodiophora brassicae produced on a non-host plant. Eur J Plant Pathol, 2012,132:309-315
[8]	Sharma K, Gossen B D , McDonald M R . Effect of temperature on cortical infection by Plasmodiophora brassicae and clubroot severity. Phytopathology, 2011,101:1424-1432
[9]	Dixon G R . Clubroot ( Plasmodiophora brassicae Woronin): an agricultural and biological challenge worldwide. Can J Plant Pathol, 2014,36(suppl. 1):5-18
[10]	Dixon G R . Plasmodiophora brassicae in its environment. J Plant Growth Reg, 2009,28:212-228
[11]	Gossen B D, Deora A, Peng G, Hwang S F , McDonald M R . Effect of environmental parameters on clubroot development and the risk of pathogen spread. Can J Plant Pathol, 2014,36(suppl. 1):37-48 doi: 10.1080/07060661.2013.859635
[12]	Honig F. Der Kohlkropferreger ( Plasmodiophora brassicae Wor.) Eine Monographie. Gartenbauwissenschaft, 1931,5:116-225
[13]	Williams P H . A system for detemination of races of Plasmodiophora bassicae that infect cabbage and rutabaga. Phytopathol, 1966,56:624-626
[14]	Buczacki S T, Toxopeus H, Mattusch P, Johnston T D, Dixon G R, Hobolth L A . Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans Br Mycol Soc, 1975,65:295-303
[15]	Somé A, Manzanares M J, Laurens F, Baron F , Thomas G and Rouxel F. Variation for virulence on Brassica napus among Plasmodiophora brassicae collections from France and derived single-spore isolates. Plant Pathol, 1996,45:432-439
[16]	Kuginuki Y, Hiroaki Y, Hirai M . Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage( Brassica rapa L. spp. pekinensis). Eur J Plant Pathol, 1999,105:327-332
[17]	刘勇, 罗一帆, 黄小琴, 张蕾, 刘红雨, 张敏 . 芸薹根肿菌生理小种鉴别方法研究进展. 中国油料作物学报, 2011,33:420-426
	Liu Y, Luo Y F, Huang X Q, Zhang L, Liu H Y, Zhang M . Research progress of physiological race distinction systems on Plasmodiophora brassicae Wor. Chin J Oil Crop Sci, 2011, 33:420-426 (in Chinese with English abstract)
[18]	沈向群, 聂凯, 吴琼, 张玉光, 孟星河 . 大白菜根肿病主要生理小种种群分化鉴定初报. 中国蔬菜, 2009,1(8):59-62
	Shen X Q, Nie K, Wu Q, Zhang Y G, Meng X H . Initial research report on differentiation identification of Chinese cabbage clubroot main physiological races. China Veg, 2009,1(8):59-62 (in Chinese with English abstract)
[19]	季海雯, 任莉, 陈坤荣, 徐理, 刘凡, 孙超超, 李俊, 刘胜毅, 方小平 . 油菜根肿病病原主要生理小种和品种抗病性鉴定. 中国油料作物学报, 2013,35:301-306
	Ji H W, Ren L, Chen K R, Xu L, Liu F, Sun C C, Li J, Liu S Y, Fang X P . Identification of physiological races of clubroot and resistance of rape cultivars to Plasmodiophora brassicae. Chin J Oil Crop Sci, 2013,35:301-306 (in Chinese with English abstract)
[20]	费维新 , Hwang S F, 王淑芬, 吴晓芸, 高智谋, 李强生, 侯树敏, 荣松柏, 江莹芬, 雷伟侠, 郝仲萍, 胡宝成. 根肿菌生理小种鉴定与甘蓝型油菜品种资源的抗性评价. 中国油料作物学报, 2016,38:626-639 doi: 10.7505/j.issn.1007-9084.2016.05.013
	Fei W X, Hwang S F, Wang S F, Wu X Y, Gao Z M, Li Q S, Hou S M, Rong S B, Jiang Y F, Lei W X, Hao Z P, Hu B C . Pathotype identification of Plasmodiophora brassicae and resistance of rapeseed variety resources to clubroot disease. Chin J Oil Crop Sci, 2016,38:626-639 (in Chinese with English abstract) doi: 10.7505/j.issn.1007-9084.2016.05.013
[21]	Jones D R, Ingram D S, Dixon G R . Factors affecting tests for differential pathogenicity in populations of Plasmodiophora brassicae. Plant Pathol, 1982,31:229-238
[22]	李茜, 沈向群, 耿新翠, 李林 . 芸薹根肿菌( Plasmodiophora brassicae)单孢分离接种及生理小种的鉴定. 植物保护, 2012,38(3):95-101
	Li Q, Shen X Q, Geng X C, Li L . Separate inoculation of single resting spores and identification of Plasmodiophora brassicae races. Plant Prot, 2012,38(3):95-101 (in Chinese with English abstract)
[23]	Gossen B D, Kasinathan H, Cao T, Manolii V P, Strelkov S E, Hwang S F , McDonald M R . Influence of pH and temperature on infection and symptom development of clubroot in canola. Can J Plant Pathol, 2013,35:294-303 doi: 10.1080/07060661.2013.804882
[24]	Friberg H, Lagerlof J, Ramert B . Germination of Plasmodiophora brassicae resting spores stimulated by a non-host plant. Eur J Plant Pathol, 2005,113:275-281
[25]	费维新, 王淑芬, 李强生, 吴晓芸, 陈凤祥, 侯树敏, 荣松柏, 郝仲萍, 高智谋, 胡宝成 . 冬油菜适当迟播有效减轻油菜根肿病. 中国油料作物学报, 2016,38:502-507
	Fei W X, Wang S F, Li Q S, Wu X Y, Chen F X, Hou S M, Rong S B, Hao Z P, Gao Z M, Hu B C . Reducing clubroot disease by late sowing of winter rapeseed. Chin J Oil Crop Sci, 2016,38:502-507 (in Chinese with English abstract)
[26]	杨进, 殷丽琴, 王晓, 付绍红, 王学贵, 李云, 王继胜, 邹琼, 陶兰蓉, 康泽明, 唐蓉 . 4种杀菌剂对油菜根肿病的防治潜力及对幼苗防御酶活性的影响. 中国油料作物学报, 2017,39:546-550 doi: 10.7505/j.issn.1007-9084.2017.04.017
	Yang J, Yin L Q, Wang X, Fu S H, Wang X G, Li Y, Wang J S, Zou Q, Tao L R, Kang Z M, Tang R . Preventive potential of 4 fungicides on clubroot ( Plasmodiophora brassicae) and rapeseed defense enzyme activity. Chin J Oil Crop Sci, 2017,39:546-550 (in Chinese with English abstract) doi: 10.7505/j.issn.1007-9084.2017.04.017
[27]	尚慧, 杨佩文, 董丽英, 刘树芳, 俞华根, 李家瑞 . 大白菜根肿病化学防治技术. 植物保护, 2009,35:157-159 doi: 10.3969/j.issn.0529-1542.2009.06.036
	Shang H, Yang P W, Dong L Y, Liu S F, Yu H G, Li J R . Chemical control strategy against Chinese cabbage clubroot. Plant Prot, 2009,35:157-159 (in Chinese with English abstract) doi: 10.3969/j.issn.0529-1542.2009.06.036
[28]	陈坤荣, 任莉, 刘凡, 徐理, 孙超超, 方小平 . 三种杀菌剂防治油菜根肿病技术研究. 中国油料作物学报, 2013,35:424-427
	Chen K R, Ren L, Liu F, Xu L, Sun C C, Fang X P . Controlling effects of three fungicides on rapeseed clubroot. Chin J Oil Crop Sci, 2013,35:424-427 (in Chinese with English abstract)
[29]	Peng G, Lahlali R, Hwang S F . Crop rotation, cultivar resistance, and fungicides/biofungicides for managing clubroot ( Plasmodiophora brassicae) on canola. Can J Plant Pathol, 2014,36(suppl. 1):99-112
[30]	Peng G, Pageau D, Strelkov S E, Lahlali R, Hwang S F, Hynes R K . Assessment of crop rotation, cultivar resistance and Bacillus subtilis biofungicide for control of clubroot on canola. Acta Hort, 2013,1005(suppl. 2-3):591-598
[31]	Hwang S F, Howard R J, Strelkov S E, Gossen B D, Peng G . Management of clubroot, on canola, in western Canada. Can J Plant Pathol, 2014,36(suppl):49-65 doi: 10.1080/07060661.2013.863806
[32]	Yoshikawa H . Studies on breeding of clubroot resistance in cole crops. Bull Natl Res Inst Veg Ornam Plants Tea, 1993,A7:1-165
[33]	Peng G, Falk K C, Gugel R K, Franke G, Yu F, James B, Strelkov S E, Hwang S F , McGregor L. Sources of resistance to Plasmodiophora brassicae( clubroot) pathotypes virulent on canola. Can J Plant Pathol, 2014,36 doi: 10.1080/07060661.2013.863805
[34]	刘勇, 黄小琴, 柯绍英, 刘红雨 . 四川主栽油菜品种根肿病抗性研究 . 中国油料作物学报, 2009,31:90-93
	Liu Y, Huang X Q, Ke S Y, Liu H Y . Evaluation of resistance of rapeseed varieties to clubroot infected by Plasmodiophora brassicae in Sichuan. Chin J Oil Crop Sci, 2009,31:90-93 (in Chinese with English abstract)
[35]	Manzanares-Dauleux M J, Deloureme R, Baron F, Thomas G . Mapping of one major gene and of QTL involved in resistance to clubroot in Brassica napus. Theor Appl Genet, 2000,101:885-891
[36]	Werner S, Diederichsen E, Frauen M, Schondelmaier J, Jung C . Genetic mapping of clubroot resistance genes in oilseed rape. Theor Appl Genet, 2008,116:363-372 doi: 10.1007/s00122-007-0674-2 pmid: 18040658
[38]	Crisp P, Crute I R, Sutherland R A, Angell S M, Bloor K, Burgess H, Gordon P L . The exploitation of genetic resources of Brassica oleracea in breeding for resistance to clubroot( Plasmodiophora brassicae). Euphytica, 1989,42:215-226
	Manzanares-Dauleux M J, Divaret I, Baron F, Thomas G . Evaluation of French Brassica oleracea landraces for resistance to Plasmodiophora brassicae. Euphytica, 2000,113:211-218
[39]	司军, 李成琼, 宋洪元, 任雪松, 宋明, 王小佳 . 结球甘蓝对根肿病的抗性鉴定与评价. 西南大学学报: 自然科学版, 2009,31(6):26-30
	Si J, Li C Q, Song H Y, Ren X S, Song M, Wang X J . Identification and evaluation of resistance to clubroot in cabbage ( Brassica oleracea var. capitata L.) . J Southwest Univ ( Nat Sci Edn), 2009,31(6):26-30 (in Chinese with English abstract)
[40]	Kamei A, Tsuro M, Kubo N , Hayashi T Wang N, Fujimura T, Hirai M. QTL mapping of clubroot resistance in radish ( Raphanus sativus L.). Theor Appl Genet, 2010,1209:1021-1027
[41]	Chen H G, Wu J S . Characterization of fertile amphidiploid between Raphanus sativus and Brassica alboglabra and the crossability with Brassica species. Genet Resour Crop Evol, 2008,55:143-150
[42]	Fuchs H, Sacristán M D . Identification of a gene in Arabidopsis thaliana controlling resistance to clubroot( Plasmodiophora brassicae) and characterization of the resistance response. Mol Plant Microbe Interact, 1996,9:91-97
[43]	Nagaoka T, Doullah M A, Matsumoto S, Kawasaki S, Ishikawa T, Hori H . Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea. Theor Appl Genet, 2010,120:1335-1346
[44]	Tomita H, Shimizu M, Doullah M A, Fujimoto R , Okazaki K. Accumulation of quantitative trait loci conferring broadspectrum clubroot resistance in Brassica oleracea. Mol Breed, 2013, 32:889-900
[45]	Voorrips R E, Jongerius M C, Kanne H J . Mapping of two genes for resistance to clubroot ( Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet, 1997,94:75-82
[46]	Lee J, Izzah N K, Choi B S, Joh H J, Lee S C, Perumal S . Genotyping-by-sequencing map permits identification of clubroot resistance QTLs and revision of the reference genome assembly in cabbage ( Brassica oleracea L.). DNA Res, 2015,23:29-41
[47]	Matsumoto E, Yasui C, Ohi M, Tsukada M . Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage ( Brassica rapa ssp. pekinensis). Euphytica, 1998,104:79-86
[48]	Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M . Identification of two loci for resistance to clubroot ( Plasmodiophora brassicae Woronin) in Brassica rapa L . Theor Appl Genet, 2003,107:997-1002
[49]	Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S . Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance. Genetics, 2006,173:309-319
[50]	Suwabe K, Suzuk G, Nunome T, Hatakeyama K, Mukai Y, Fukuoka H, Matsumoto S . Microstructure of a Brassica rapa genome segment homologous to the resistance gene cluster on Arabidopsis chromosome 4. Breed Sci, 2012,62:170-177
[51]	Hatakeyama K, Suwabe K, Tomita R N, Kato T, Nunome T, Fukuoka H, Matsumoto S . Identification and characterization of Crr1a, a gene for resistance to clubroot disease( Plasmodiophora brassicae Woronin) in Brassica rapa L . PLoS One, 2013, 8:e54745
[52]	Hirai M, Harada T, Kubo N, Tsukada M, Suwabe K, Matsumoto S . A novel locus for clubroot resistance in Brassica rapa and its linkage markers. Theor Appl Genet, 2004,108:639-643 doi: 10.1007/s00122-003-1475-x pmid: 14551685
[53]	Piao Z Y, Deng Y Q, Choi S R, Park Y J, Lim Y P . SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage( Brassica rapa ssp. pekinensis). Theor Appl Genet, 2004,108:1458-1465
[54]	Sakamoto K, Saito A, Hayashida N, Taguchi G, Matsumoto E . Mapping of isolate-specific QTL for clubroot resistance in Chinese cabbage ( Brassica rapa L. ssp. pekinensis). Theor Appl Genet, 2008,117:759-767
[55]	Chen J, Jing J, Zhan Z, Zhang T, Zhang C, Piao Z . Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa. PLoS One, 2013,8:e85307
[56]	Chu M, Song T, Falk K C, Zhang X, Liu X, Chang A , Lahlali, R, McGregor L, Gossen B D, Yu F, Peng G. Fine mapping of Rcr1 and analyses of its effect on transcriptome patterns during infection by Plasmodiophora brassicae. BMC Genomics, 2014,15:1166
[57]	Yu F, Zhang X, Peng G, Falk K C, Strelkov S E, Gossen B D . Genotyping-by-sequencing reveals three QTL for clubroot resistance to six pathotypes of Plasmodiophora brassicae in Brassica rapa. Sci Rep, 2017,7:4516
[58]	Saito M, Kubo N, Matsumoto S, Suwabe K, Tsukada M, Hirai M . Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa. Theor Appl Genet, 2006,114:81-91
[59]	Zhang T, Zhao Z, Zhang C, Pang W, Choi S R, Lim Y P, Piao Z . Fine genetic and physical mapping of the CRb gene conferring resistance to clubroot disease in Brassica rapa. Mol Breed, 2014,34:1173-1183
[60]	Kato T, Hatakeyama K, Fukino N, Matsumoto S . Fine mapping of the clubroot resistance gene CRb and development of a useful selectable marker in Brassica rapa. Breed Sci, 2013,63:116-124
[61]	Zhang H, Feng J, Hwang S H, Strelkov S E, Falak I, Huang X, Sun R . Mapping of clubroot ( Plasmodiophora brassicae) resistance in canola( Brassica napus). Plant Pathol, 2016,65:435-440
[62]	战宗祥, 江莹芬, 朱紫媛, 张春沙, 杨庆勇, 李倩, 侯照科, 龚建芳, 程雨贵, 吴江生, 傅廷栋, 周永明, 朴钟云, 张椿雨 . 与位点 PbBa8.1紧密连锁分子标记的开发及甘蓝型油菜根肿病抗性育种. 中国油料作物学报, 2015,37:772-779 doi: 10.7505/j.issn.1007-9084.2015.06.005
	Zhan Z X, Jiang Y F, Zhu Z Y, Zhang C S, Yang Q Y, Li Q, Hou Z K, Gong J F, Cheng Y G, Wu J S, Fu T D, Zhou Y M, Piao Z Y, Zhang C Y . Development of close linked marker to PbBa8.1 conferring canola resistance to Plasmodiophora brassicae. Chin J Oil Crop Sci, 2015,37:772-779 (in Chinese with English abstract) doi: 10.7505/j.issn.1007-9084.2015.06.005
[63]	Pang W, Fu P, Li X, Zhan Z, Yu S, Piao Z . Identification and Mapping of the Clubroot Resistance Gene CRd in Chinese Cabbage( Brassica rapa ssp. pekinensis). Front Plant Sci, 2018,9:653
[64]	Ueno H, Matsumoto E, Aruga D, Kitagawa S, Matsumura H, Hayashida N . Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa. Plant Mol Biol, 2012,80:621-629 doi: 10.1007/s11103-012-9971-5 pmid: 23054353
[65]	Hatakeyama K, Niwa T, Kato T, Ohara T, Kakizaki T, Matsumoto S . The tandem repeated organization of NB-LRR genes in the clubroot-resistant CRb locus in Brassica rapa L . Mol Genet Genomics, 2017,292:397-405
[66]	Jones J D, Dangl J L . The plant immune system. Nature, 2006,444:323-329 doi: 10.1038/nature05286
[67]	Chen J, Pang W, Chen B, Zhang C, Piao Z . Transcriptome analysis of Brassica rapa Near-Isogenic Lines carrying clubroot- resistant and -susceptible alleles in response to Plasmodiophora brassicae during early infection. Front Plant Sci, 2016,6:1183
[68]	Yu F, Zhang X, Huang Z, Chu M, Song T, Falk K, Deora A, Chen Q, Zhang Y , McGregor L, Gossen B D, McDonald M R, Peng G. Identification of genome-wide variants and discovery of variants associated with Brassica rapa clubroot resistance gene Rcr1 through bulked segregant RNA sequencing. PLoS One, 2016,11:e0153218
[69]	Song T, Chu M, Lahlali R, Yu F, Peng G . Shotgun Label-free proteomic analysis of clubroot ( Plasmodiophora brassicae) resistance conferred by the gene Rcr1 in Brassica rapa. Front Plant Sci, 2016,7:1013
[70]	Li H, Li X, Xuan Y, Jiang J, Wei Y, Piao Z . Genome wide identification and expression profiling of SWEET genes family reveals its role during Plasmodiophora brassicae-induced formation of clubroot in Brassica rapa. Front Plant Sci, 2018,9:207
[71]	Matsumoto E, Ueno H, Aruga D, Sakamoto K, Hayashida N . Accumulation of three clubroot resistance genes through marker-assisted selection in Chinese cabbage ( Brassica rapa ssp. pekinensis). J Jpn Soc Hortic Sci, 2012,81:184-190
[72]	Diederichsen E, Beckmann J, Schondelmeier J, Dreyer F . Genetics of clubroot resistance in Brassica napus ‘Mendel’. Acta Hort, 2006,706:307-312
[73]	Diederichsen E, Frauen M, Linders E G, Hatakeyama K, Hirai M . Status and perspectives of clubroot resistance breeding in crucifer crops. J Plant Growth Regul, 2009,28:265-281 doi: 10.1007/s00344-009-9100-0
[74]	Zhan Z, Nwafor C C, Hou Z, Gong J, Zhu B, Jiang Y, Zhou Y, Wu J, Piao Z, Tong Y, Liu C, Zhang C . Cytological and morphological analysis of hybrids between Brassica raphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L. PLoS One, 2017,12:e0177470
[75]	Matsumoto E, Ueno H, Aruga D, Sakamoto K, Hayashida N . Accumulation of three clubroot resistance genes through marker-assisted selection in Chinese cabbage ( Brassica rapa ssp. pekinensis). Engei Gakkai Zasshi, 2012,81:184-190
[76]	Diederichsen E, Frauen M, Ludwig-Müller J . Clubroot disease management challenges from a German perspective. Can J Plant Pathol, 2014,36(suppl. 1):85-98 doi: 10.1080/07060661.2013.861871

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