甘蓝型油菜皖油20号种子不同部位油脂合成的转录调控分析

doi:10.3724/SP.J.1006.2019.84105

Abstract

Abstract:

Brassica napus is one of the main oil crops and the seed oil content is generally between 35% and 50%. Oil is mainly stored in the seed embryo. Embryo is composed of cotyledons (including outer and inner cotyledons) and embryonic axis. The oil content and fatty acid composition of different parts of low erucic Brassica napus WY20’s seed were analyzed. There was a significant difference in oil content in different parts of the seed. The oil content in the outer cotyledon was the highest while embryonic axis had the lowest oil content. At the same time, the fatty acid composition also showed significant difference in different parts of the seed. The ratio of C16:0, C18:2, and C20:0 in embryonic axis was significantly higher than that in cotyledon. The ratio of C16:0 in the embryonic axis was about twice more than that of the cotyledons. The ratio of C18:1 and C20:1 in cotyledons was significantly higher than that in embryonic axis. C18:0 had the lowest content in the outer cotyledon and no difference in the inner cotyledons and embryonic axis. C18:3 had the highest content in the outer cotyledons and no difference between inner cotyledons and embryonic axis. Transcriptome analysis was performed for the inner cotyledon, outer cotyledon and embryonic axis of the 34-day-old seed. A total of 7192 differentially expressed genes (DEGs) were identified after pairwise comparison of gene expression of the three parts. There were much fewer DEGs between cotyledons and more DEGs between cotyledon and embryonic axis. These DEGs were enriched in biological processes such as photosynthesis, fatty acid metabolism and chlorophyll metabolism. Gene function annotations revealed that there were 355 genes involved in lipid metabolism, especially in the de novo fatty acid biosynthesis in plastid. This study suggests that transcriptional regulation of key genes involved in oil biosynthesis results in different oil contents and fatty acid compositions in different parts of seed in Brassica napus.

Key words: Brassica napus, different seed parts, oil content, fatty acid composition, transcriptional regulation

Yu-Ting ZHANG,Shao-Ping LU,Cheng JIN,Liang GUO. Transcriptional regulation of oil biosynthesis in different parts of Wanyou 20 (Brassica napus) seeds[J].Acta Agronomica Sinica, 2019, 45(3): 381-389.

Figures/Tables 5

Fig. 1

Fig. 2

Fig. 3

Table 1

Differentially expressed oil biosynthesis-related genes in different seed tissues of WY20"

基因名 Gene ID	注释描述 Annotation description	蛋白家族缩写 Protein family abbreviations		分组 Group
BnaA03g13590D	Fatty acid desaturase 3	FAD3		EA-IC-OC
BnaC03g16520D	Fatty acid desaturase 3	FAD3		EA-IC/EA-OC
BnaC04g14820D	Fatty acid desaturase 3	FAD3		EA-IC/EA-OC
BnaC04g40760D	Fatty acid desaturase 3	FAD3		EA-IC/EA-OC
BnaA04g17150D	Fatty acid desaturase 3	FAD3		EA-IC/EA-OC
BnaAnng09250D	Fatty acid desaturase 2	FAD2		EA-IC/EA-OC
BnaA02g11570D	Hydroxysteroid dehydrogenase 1	OBO		EA-IC-OC
BnaA03g23490D	Hydroxysteroid dehydrogenase 1	OBO		EA-IC-OC
BnaC03g27860D	Hydroxysteroid dehydrogenase 1		OBO	EA-IC-OC
BnaCnng57830D	Hydroxysteroid dehydrogenase 1		OBO	EA-IC
BnaA09g02110D	Oleosin		OBO	EA-OC
BnaA03g20420D	Stearoyl-acyl-carrier-protein desaturase protein		SAD	EA-IC/EA-OC
BnaA01g32860D	Stearoyl-acyl-carrier-protein desaturase protein		SAD	EA-IC/EA-OC
BnaC03g24420D	Stearoyl-acyl-carrier-protein desaturase protein		SAD	EA-IC/EA-OC
BnaC09g41580D	Stearoyl-acyl-carrier-protein desaturase protein		SAD	EA-IC/EA-OC
BnaA10g18080D	Stearoyl-acyl-carrier-protein desaturase protein		SAD	EA-IC/EA-OC
BnaA05g03490D	Stearoyl-acyl-carrier-protein desaturase		SAD	EA-OC
BnaC04g03030D	Stearoyl-acyl-carrier-protein desaturase		SAD	EA-OC
BnaC09g19280D	3-ketoacyl-acyl carrier protein synthase I		KASI	EA-IC/EA-OC
BnaA02g24400D	3-ketoacyl-acyl carrier protein synthase I		KASI	EA-IC/EA-OC
BnaA06g36060D	3-ketoacyl-acyl carrier protein synthase I		KASI	EA-IC/EA-OC
BnaC06g35760D	3-ketoacyl-acyl carrier protein synthase II		KAS II	EA-IC/EA-OC
BnaA07g31890D	3-ketoacyl-acyl carrier protein synthase II		KAS II	EA-IC/EA-OC
BnaA07g21940D	3-ketoacyl-acyl carrier protein synthase II		KAS II	EA-IC/EA-OC
BnaC06g22680D	3-ketoacyl-acyl carrier protein synthase II		KAS II	EA-IC
BnaA04g07120D	Acyl-ACP thioesterase		FATA	EA-IC/EA-OC
BnaCnng41490D	Acyl-ACP thioesterase		FATA	EA-IC/EA-OC
BnaCnng00070D	FATA acyl-ACP thioesterase FATA		FATA	EA-IC/EA-OC
BnaA07g05070D	FATA acyl-ACP thioesterase FATA		FATA	EA-IC/EA-OC
BnaC03g75820D	Ketoacyl-ACP Reductase		KAR	EA-IC/EA-OC
BnaA02g13310D	Beta-ketoacyl reductase		KAR	EA-IC/EA-OC
BnaA07g26670D	Beta-ketoacyl reductase		KAR	EA-IC/EA-OC
BnaC06g28830D	Beta-ketoacyl reductase		KAR	EA-IC/EA-OC
BnaC09g16320D	Acyl carrier protein		ACP	EA-IC/EA-OC
BnaC09g03000D	Acyl carrier protein		ACP	EA-IC/EA-OC
BnaA09g03610D	Acyl carrier protein		ACP	EA-IC/EA-OC
BnaAnng23710D	Acyl carrier protein		ACP	EA-OC
BnaC03g45040D	Enoyl-ACP Reductase		ENR	EA-IC/EA-OC
BnaC07g04330D	Enoyl-ACP Reductase		ENR	EA-IC/EA-OC
BnaA03g38220D	Enoyl-ACP Reductase		ENR	EA-IC/EA-OC
BnaAnng02240D	E2 component of pyruvate dehydrogenase complex		DHLAT	EA-IC/EA-OC
BnaC06g08280D	E2 component of pyruvate dehydrogenase complex		DHLAT	EA-IC/EA-OC
BnaC07g23030D	E2 component of pyruvate dehydrogenase complex		DHLAT	EA-IC
BnaA06g33300D	E2 component of pyruvate dehydrogenase complex		DHLAT	EA-IC
BnaAnng22560D	Chloroplasticacetyl coenzyme A carboxylase		BCCP	EA-IC/EA-OC
BnaC09g42420D	Biotin carboxyl carrier protein 2		BCCP	EA-IC/EA-OC
BnaA03g02830D	Thioesterase superfamily protein		HAD	EA-IC/EA-OC
BnaA02g00390D	Thioesterase superfamily protein		HAD	EA-IC
BnaA07g20920D	Long chain acyl-CoA synthetase 9		LACS9	EA-IC/EA-OC
BnaC06g20910D	Long chain acyl-CoA synthetase 9		LACS9	EA-IC
BnaA01g17630D	E3 component of pyruvate dehydrogenase complex		LPD	EA-IC/EA-OC
BnaCnng75250D	Acetyl-CoA carboxylase		ACCase	EA-IC
BnaA05g12180D	Acyl-carrier-protein		MCMT	EA-IC/EA-OC

Table 1

Fig. 4

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Transcriptional regulation of oil biosynthesis in different parts of Wanyou 20 (Brassica napus) seeds

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