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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (5): 725-733.doi: 10.3724/SP.J.1006.2020.94145

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Controlling efficiency against clubroot disease of rapeseed by mixed-cropping of susceptible and resistant cultivars

Qing-Yun GUO1,Bo WANG1,*(),Jie KUAI1,Chun-Yu ZHANG1,Gen-Ze LI2,Hui-Xian KANG3,Ting-Dong FU1,Guang-Sheng ZHOU1   

  1. 1Key Laboratory of Crop Physiology, Ecology and Cultivation (The Middle Reaches of the Yangtze River) of Ministry of Agriculture / College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
    2Industrial Crop Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
    3Linxiang Agricultural Technique Extension Station, Lincang City, Linxiang 677000, Yunnan, China
  • Received:2019-09-28 Accepted:2019-12-26 Online:2020-05-12 Published:2020-01-13
  • Contact: Bo WANG E-mail:wangbo@mail.hzau.edu.cn
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2018YFD1000900);the Technological Innovation Major Project of the Science and Technology Department of Hubei Province(2017ABA064);and the Fundamental Research Funds for the Central Universities.(2662017JC005)

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Abstract:

In recent years, rapeseed clubroot disease has spread widely in China, and the cost of the chemical control is high while the efficiency is low. Using resistant varieties is the most economical and efficient way to control this disease. Mixed-cropping different resistant varieties can improve the control efficiency of crop diseases, but the research on it has not been reported. In two naturally-infested locations of clubroot diseases in Jixi of Anhui province and Linxiang of Yunnan province, two pairs of susceptible and resistant cultivars were used (highly resistant variety Huashuang 5R mixed with highly susceptible variety Huashuang 5 and highly resistant variety Huayouza 62R mixed with highly susceptible variety Huayouza 62). Seeds of each pair were mixed at different ratios (10:0, 1:9, 2:8, 3:7, and 0:10) and sown in the two locations respectively. The incidence rate was surveyed after occurrence of clubroot disease. The mixed-cropping at different ratios of susceptible and resistant cultivars decreased the incidence and disease index of clubroot disease, and stabilized the yield and quality of rapeseed. The actual incidence rate in the mixed cropping treatment increased with the decrease of resistant cultivar proportion, which was from 5.7% to 17.1% at Jixi and from 4.4% to 32.1% at Linxiang for Huashuang 5R/Huashang 5, while from 5.7% to 18.6% at Jixi and from 2.1% to 12.0% at Linxiang for Huayouza 62R/Huayouza 62 respectively, and significantly lower than the theoretical disease incidence rate. The relative effectiveness of mixture of the two pairs of cultivars were lower than 1, which indicates that the mixed-cropping of susceptible and resistant cultivars has positive effect. This study mainly clarified the control effect of mixed-cropping two different Near-isogenic lines with different resistance levels on clubroot disease, which can provide new ideas and countermeasures for the prevention of clubroot disease.

Key words: rapeseed, clubroot disease, near-isogenic lines, mixed-cropping

Table 1

Incidence under different treatments in different plaeas"

地点
Place		 组合
Combination		 比例
Ratio		 发病率A
Incidence rateA (%)		 病情分级A
Disease scaleA		 病情
指数A
Disease indexA		 发病率B
Incidence rateB (%)		 病情分级B
Disease scaleB		 病情
指数B
Disease indexB
理论值Theoretical 实际值Actual 0 1 2 3 理论值Theoretical 实际值
Actual		 0 1 2 3
绩溪
Jixi		 华双5号:华双5 R
Huashuang 5(S): Huashuang 5R(R)		 0:10 0 0 c 30 0 0 0 0 d 0.3 0.3 b 30 0 0 0 0 d
1:9 10.0 5.7 c 28 0 2 0 4.4 c 10.3 0.6 b 28 0 1 1 5.6 b
2:8 20.0 16.1 b 25 1 1 3 13.3 b 20.2 0.8 b 28 1 0 1 4.4 c
3:7 30.0 17.1 b 25 10 3 1 11.1 b 30.2 1.4 b 29 0 0 1 3.3 c
10:0 100.0 100.0 a 0 0 2 28 97.8 a 100.0 100.0 a 0 0 0 30 100.0 a
理论值Theoretical 实际值Actual 0 1 2 3 理论值Theoretical 实际值
Actual		 0 1 2 3
华油杂62号:
华油杂62 R
Huayouza 62(S): Huayouza 62R(R)		 0:10 0 0 e 30 0 0 0 0 e 0 0 d 30 0 0 0 0 d
1:9 10.0 5.7 d 28 1 1 0 3.3 d 10.0 3.6 c 29 0 1 0 2.2 c
2:8 20.0 10.9 c 27 2 1 0 4.4 c 20.0 4.1 c 29 0 1 0 2.2 c
3:7 30.0 18.6 b 25 4 1 1 10.0 b 30.0 12.4 b 27 2 1 0 4.5 d
10:0 100.0 100.0 a 0 0 0 30 100.0 a 100.0 100.0 a 0 0 0 30 100.0 a
临翔
Linxiang		 华双5号:华双5 R Huashuang 5(S): Huashuang 5R(R) 0:10 2.0 2.0 c 29 1 0 0 1.0 c 2.2 2.2 b 29 0 0 1 3.3 d
1:9 5.0 4.4 bc 28 0 1 1 5.6 b 8.1 4.4 b 29 0 0 1 3.3 d
2:8 8.0 7.2 bc 28 0 0 2 6.7 b 14.0 7.8 b 28 0 0 2 6.7 c
3:7 11.0 10.1 b 27 1 1 1 6.7 b 20.0 11.1 b 27 0 1 2 8.9 b
10:0 32.1 32.1 a 20 0 0 10 33.3 a 61.1 61.1 a 12 0 1 17 58.9 a
华油杂62号:
华油杂62 R
Huayouza 62(S): Huayouza 62R(R)		 0:10 0 0 e 30 0 0 0 0 e 8.4 8.4 d 27 1 2 0 5.6 d
1:9 7.7 2.1 d 29 1 0 0 1.1 d 16.5 12.0 c 26 2 1 1 7.8 c
2:8 15.5 8.0 c 28 2 0 0 2.2 c 24.5 13.4 c 26 1 2 1 8.9 b
3:7 23.3 12.0 b 26 3 1 0 5.6 b 32.6 16.0 b 25 3 1 1 8.9 b
10:0 77.5 77.5 a 7 0 1 22 75.6 a 88.9 88.9 a 3 0 27 27 90.0 a

Table 2

Relative effectiveness of mixture (REM) under different treatments"

组合
Combination		 比例
Ratio		 绩溪Jixi 临翔 Linxiang
苗期Seedling 蕾薹期Budding 蕾薹期Budding 成熟期Maturation
华双5号:华双5 R Huashuang 5(S): Huashuang 5R(R) 0:10 — — — —
1:9 0.57 0.06 0.88 0.54
2:8 0.81 0.04 0.90 0.56
3:7 0.57 0.05 0.92 0.56
10:0 — — — —
华油杂62号:华油杂62 R
Huayouza 62(S):
Huayouza 62R(R)		 0:10 — — — —
1:9 0.57 0.36 0.27 0.73
2:8 0.56 0.21 0.52 0.55
3:7 0.62 0.41 0.52 0.50
10:0 — — — —

Table 3

Yield and yield components under different treatments in Jixi and Linxiang"

组合
Combination		 地点
Place		 比例
Proportion		 产量
Yield
(kg hm-2)		 实收密度
Density (×104 plants hm-2)		 单株产量
Yield per plant
(g plant-1)		 单株角果数
Silique no.
per plant		 每角果粒数
Seed no. per silique		 千粒重
1000-seed weight (g)
华双5号:华双5号R
Huashuang 5(S): Huashuang 5R(R)		 绩溪
Jixi		 0:10 2719.5 a 28.1 a 11.5 b 176.0 b 18.8 b 3.48 d
1:9 2545.5 ab 26.9 a 12.6 ab 181.7 ab 19.9 a 3.53 c
2:8 2506.5 ab 22.7 b 13.5 a 187.3 ab 20.0 a 3.61 a
3:7 2466.0 b 22.1 b 13.7 a 192.7 a 20.1 a 3.58 b
10:0 0 c 0 c 0 c 0 c 0 c 0 e
临翔Linxiang 0:10 3019.5 a 30.1 a 10.8 c 187.0 d 19.6 b 2.95 b
1:9 2981.4 a 29.2 a 11.2 b 194.7 b 20.1 ab 2.87 b
2:8 2938.2 a 26.7 b 14.3 a 190.7 c 20.6 ab 3.63 a
3:7 2846.7 a 26.0 b 14.5 a 202.3 a 21.0 a 3.41 a
10:0 2579.1 b 24.7 c 10.6 c 149.7 e 19.4 b 3.65 a
华油杂62号:华油杂62R
Huayouza 62(S):
Huayouza 62R(R)		 绩溪
Jixi		 0:10 3383.3 a 25.7 a 13.2 b 180.7 b 20.6 c 3.54 b
1:9 3142.2 ab 22.3 b 14.1 a 186.5 a 21.2 b 3.57 b
2:8 2904.4 b 20.8 b 13.9 ab 176.0 c 21.6 b 3.68 a
3:7 3092.2 b 20.7 b 14.9 a 180.0 b 22.7 a 3.66 a
10:0 0 c 0 c 0 c 0 d 0 d 0 c
临翔
Linxiang		 0:10 3413.7 a 31.5 a 10.8 b 180.7 c 19.4 a 3.08 b
1:9 3158.8 b 28.1 b 11.3 a 191.7 a 19.2 a 3.07 b
2:8 3058.1 b 28.5 b 10.7 b 184.5 b 19.2 a 3.02 c
3:7 2776.3 c 28.0 b 9.9 c 177.2 d 18.5 b 3.02 c
10:0 2527.3 d 26.6 b 9.5 c 160.5 e 18.5 b 3.20 a

Table 4

Difference of rapeseed quality under different treatments in Jixi and Linxiang (%)"

组合
Combination		 比例
Ratio		 绩溪 Jixi 临翔 Linxiang
含油量
Oil content		 蛋白质
Protein		 含油量
Oil content		 蛋白质
Protein
华双5号:华双5号R
Huashuang 5(S) : Huashuang 5R(R)		 0:10 48.30 a 21.17 a 44.16 b 23.25 a
1:9 49.31 a 20.71 a 44.59 ab 22.44 ab
2:8 49.05 a 19.22 a 45.65 a 21.96 b
3:7 48.61 a 19.00 a 44.60 ab 21.98 b
10:0 — — 44.88 ab 22.60 ab
华油杂62号:华油杂62 R
Huayouza 62(S) : Huayouza 62R(R)		 0:10 45.12 a 20.26 a 42.04 b 25.21 a
1:9 45.05 a 20.24 a 42.20 ab 24.08 a
2:8 45.91 a 19.15 a 42.31 ab 24.34 a
3:7 46.22 a 19.06 a 43.22 a 24.28 a
10:0 — — 43.07 ab 24.33 a

Table 5

Amino acid content in rapeseed under different treatments for Huashuang 5(S):Huashuang 5R(R) "

氨基酸
Amino acid		 绩溪Jixi 临翔Linxiang
比例Ratio 变异系数
CV (%)		 比例Ratio 变异系数
CV (%)
0:10 3:7 0:10 3:7 10:0
中性 Phe 0.82 0.80* 1.51 0.90 ab 0.86 b 0.92 a 3.51
Neutral Lle 0.81 0.78* 2.07 0.85 ab 0.82 b 0.88 a 3.87
Thr 0.75 0.70* 3.58 0.73 b 0.67 c 0.85 a 10.27
Ser 0.61 0.56* 6.11 0.53 b 0.44 b 0.74 a 22.50
Met 0.25 0.23* 3.49 0.23 b 0.24 b 0.25 a 3.15
Val 1.03 1.00* 1.85 1.14 a 1.12 a 1.15 a 2.73
Leu 1.26 1.24 1.20 1.40 ab 1.33 b 1.44 a 4.54
Tyr 0.52 0.52 1.60 0.52 a 0.48 c 0.60 a 9.61
Pro 1.19 1.19 1.92 1.40 a 1.30 b 1.33 b 3.76
Gly 1.02 1.00 1.08 1.11 a 1.07 a 1.10 a 2.88
Ala 0.85 0.84 1.54 0.96 a 0.93 a 0.95 a 2.18
碱性 Lys 1.38 1.35* 1.20 1.51 a 1.40 b 1.53 a 4.62
Basic Arg 1.22 1.16* 2.41 1.43 a 1.34 b 1.46 a 3.71
His 0.61 0.57* 3.48 0.64 a 0.62 a 0.63 a 5.42
酸性 Glu 3.37 3.46 3.00 3.89 a 3.65 b 3.65 b 3.72
Acidic Asp 1.36 1.29* 2.69 1.55 a 1.49 a 1.54 a 2.72
总量Total 17.00 16.66 — 18.74 17.74 18.97 —
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