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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (1): 116-124.doi: 10.3724/SP.J.1006.2021.04087

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of calcium application on the structural diversity of endophytic bacterial community in peanut roots under acidic red soil cultivation

ZHANG Wei1(), HONG Yan-Yun1, LIU Deng-Wang2, ZHANG Bo-Wen2, YI Tu-Yong1,*(), LI Lin2,*()   

  1. 1College of Plant Protection, Hunan Agricultural University / Hunan Provincial Key Laboratory for Biology and Control of Plant Pests, Changsha 410128, Hunan, China
    2Institute of Dryland Crops of Hunan Agricultural University, Changsha 410128, Hunan, China
  • Received:2020-04-02 Accepted:2020-09-13 Online:2021-01-12 Published:2020-09-22
  • Contact: YI Tu-Yong,LI Lin E-mail:349187910@qq.com;yituyong@hunau.net;lilindw@163.com
  • Supported by:
    National Research and Development Program of China(2018YFD1000900)

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Abstract:

Peanut is an important economic crop and calcium-loving crop in China. In order to explore the effect of calcium application on the diversity of endophytic bacteria in peanut roots planted in acid red soil, the genomes of endophytic bacteria in peanut roots of different treatments and different growth stages were deeply sequenced by 16SrRNA gene V3-V4 region, and the root microbial community structure of peanut plants planted in acid red soil and control was analyzed. The results showed that Klebsiella and Enterobacter were the dominant genera with high relative abundance in the endophytic bacterial community from all sample groups. The relative abundance of Pseudomonas and Lysinibacillus were significantly higher than that from the control group, while the relative abundance of Ralstonia decreased significantly in the calcium application groups during the pod-pin stage at P ≤ 0.05. The interaction network analysis showed that the connection of root endophytic bacterial in calcium application group was relatively close. We reasonably inferred that the composition of peanut root endophytic bacterial community was affected by calcium application and plant growing development. Calcium application could change the community structure and improve the ability of peanut to cope with external stress. This study may lay a foundation for improving the quality of acid soil and improving the disease resistance of peanut by applying calcium in the future.

Key words: peanut, calcium, acid red soil, endophytic bacteria, microbial diversity

Table 1

Group number of 16S amplicon sequencing sample"

生育期
Stage		 不施钙组送样编号
Sample groups without calcium application		 施钙组送样编号
Sample groups with calcium application
苗期 Seeding stage B.R.1, B.R.2, B.R.3 BC.R.1, BC.R.2, BC.R.3
花针期 Acicula forming stage F.R.1, F.R.2, F.R.3 FC.R.1, FC.R.2, FC.R.3
饱果期 Pod maturing stage P.R.1, P.R.2, P.R.3 PC.R.1, PC.R.2, PC.R.3

Fig. 1

PCR electrophoresis detection of 16S V3-V4 from endophytic bacteria genome Sample groups are the same as those given in Table 1."

Fig. 2

Venn analysis and UPGMA clustering A: Venn diagram of OTUs from the root endophytes in different sample groups; B: UPGMA clustering tree of the root endophytes in different sample groups. Sample groups are the same as those given in Table 1."

Fig. 3

Histogram of the Top 20 genera relative abundance from different sample groups Sample groups are the same as those given in Table 1."

Table 2

Percentage of the Top 10 relative abundance under different growth stage and different treatments at the genus level"

处理
Treatment		 样品编号
Sample group		 优势菌属
Dominant genus		 相对丰度百分比PRA (%) 处理
Treatment		 样品编号
Sample group		 优势菌属
Dominant genus		 相对丰度百分比PRA(%)
苗期不施钙
Seeding stage without calcium		 B.R. Acinetobacter 16.9 苗期施钙 Seeding stage with calcium
 BC.R. Klebsiella 30.1
Klebsiella 16.8 Bacillus 26.3
Enterobacter 7.5 Enterobacter 6.1
Anoxybacillus 5.0 Enhydrobacter 5.1
Herbaspirillum 4.9 Bradyrhizobium 1.8
花针期不施钙
Acicula forming stage without calcium
 F.R. Klebsiella 16.9 花针期施钙
Acicula forming stage with calcium
 FC.R. Klebsiella 37.2
Enterobacter 8.9 Stenotrophomonas 13.1
Acinetobacter 6.7 Pseudomonas 8.7
Pantoea 5.0 Comamonas 8.3
Dyella 3.7 Enterobacter 7.4
饱果期不施钙
Pod maturing stage without calcium
 P.R. Klebsiella 33.4 饱果期施钙
Pod maturing stage with calcium		 PC.R. Klebsiella 30.9
Enterobacter 11.3 Pseudomonas 8.9
Paenibacillus 6.3 Paenibacillus 8.7
Pseudomonas 4.9 Lysinibacillus 8.3
Bacillus 1.7 Bacillus 3.2

Fig. 4

Genera with significant differences in relative abundance at acicula forming stage"

Fig. 5

LEfSe analysis cladogram of endophytic bacteria from different sample groups Sample groups are the same as those given in Table 1."

Fig. 6

Interaction network of endophytic bacteria under different treatments A: interaction network of endophytic bacteria with calcium application; B: interaction network of endophytic bacteria without calcium application."

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