Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (8): 1624-1630.doi: 10.3724/SP.J.1006.2021.01069

• RESEARCH NOTES • Previous Articles    

Analysis on the accumulation characteristics of seven flavonoids at grain development stage in barley

HE Jun-Yu(), ZHONG Wei, CHEN Yun-Qiong, WANG Wei-Bin, XIONG Jing-Lei, JIANG Ya-Li, SHI Hui-Meng, CHEN Sheng-Wei*()   

  1. Collage of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2020-08-28 Accepted:2021-01-14 Online:2021-08-12 Published:2021-02-19
  • Contact: CHEN Sheng-Wei E-mail:etfiee_coisini@126.com;ynkmcsw@126.com
  • Supported by:
    National Natural Science Foundation of China(32060457)

RichHTML386

17

PDF

499

Abstract:

The analysis on the accumulation characteristics of flavonoids in barley grain is helpful for the studies, such as the content detection, accumulation mechanism and physiological function of these compounds. In this study, the contents of 15 flavonoids of eight barley material grains during seven development stages were determined by HPLC. The total contents of 15 compounds were exhibited the trend of up-down variation, reaching the highest value (335.71±41.63) μg g-1 on the 10th day after anthesis, and the lowest (165.52±38.63) μg g-1 in mature grain. The highest contents of troxerutin, apigenin, formononetin, and chrysin were (155.75±30.76) μg g-1, (8.41±1.54) μg g-1, (10.71±1.53) μg g-1, and (4.41±0.60) μg g-1 on the 10th day after anthesis, respectively. The highest contents of naringenin chalcone, dihydroquercetin, and luteolin were (42.41±15.39) μg g-1, (17.92±6.60) μg g-1, and (5.19±1.37) μg g-1 on the 5th, 15th, and 25th day after anthesis, respectively. The content of naringenin chalcone was changed with down-platform-down trend during development of barley grain, but the contents of troxerutin and chrysin were changed with up-down-platform-down trend, and the contents of dihydroquercetin, luteolin, apigenin and formononetin were changed with up-down trend. In terms of flavonoids content detection, the appropriate sampling time of for troxerutin, apigenin, formononetin and chrysin in barley grains was 10 days after post-anthesis stage, and the appropriate time of naringenin chalcone, dihydroquercetin, and luteolin was 5, 15, and 25 days after post-anthesis stage. These results can provide the theoretical support for the content detection of flavonoids in barley grains.

Key words: barley grain, development stage, flavonoids, accumulation characteristics

Table 1

Experimental materials and their basic characteristics"

材料编号
Code of materials		 材料名称
Name		 籽粒颜色
Coat color of grains		 材料类型
Material type
Yel1 Bowman 黄色籽粒Yellow grain coat 育成品种Improved variety
Yel2 Morex 黄色籽粒Yellow grain coat 育成品种Improved variety
Yel3 光头大麦Guangtoudamai 黄色籽粒Yellow grain coat 地方品种Landrace
Pur1 淳安六棱胭脂大麦Chunanliulengyanzhidamai 紫色籽粒Purple grain coat 地方品种Landrace
Pur2 紫青稞Purple highland barley 紫色籽粒Purple grain coat 育成品种Improved variety
Pur3 定海红绿黄Dinghaihonglyuhuang 紫色籽粒Purple grain coat 地方品种Landrace
Bla 云南高世代Yunnan gaoshidai 黑色籽粒Black grain coat 育成品系Developed line
Blu 北青7号Beiqing 7 蓝色籽粒Blue grain coat 育成品系Improved variety

Fig. 1

Total content curve of 15 compounds during seven development stages in barley grain. T1-T7: on the 5th, 10th, 15th, 20th, 25th, 30th day after anthesis, and at mature stage, respectively."

Table 2

Average contents and the differences of specific compounds at different developmental stages"

化合物
Compound		 T1 T2 T3 T4 T5 T6 T7
曲克芦丁
Troxerutin		 91.75±23.78
bc ABC		 155.75±30.76
a A		 134.88±24.36
ab AB		 65.23±27.44
cd B		 56.07±12.95
cd BC		 53.42±16.27
cd BC		 21.64±6.37
d C
二氢槲皮素
Dihydroquercetin		 6.52±2.37
cd BC		 21.80±5.19
a A		 17.92±6.60
ab AB		 16.48±3.97
abc ABC		 13.37±3.17
abcd ABC		 9.55±2.82
bcd ABC		 2.63±0.90
d C
木犀草素
Luteolin		 0.60±0.36
b B		 1.75±0.89
b AB		 1.32±0.68
b AB		 5.02±1.97
a A		 5.19±1.37
a A		 3.45±1.10
ab AB		 3.49±0.87
ab AB
柚皮素查尔酮
Naringenin chalcone		 42.41±15.39
a A		 11.31±6.69
b AB		 13.63±12.95
b AB		 10.23±7.89
b AB		 6.60±6.60
b B		 0.00±0.00
b B		 4.17±2.36
B B
芹菜素
Apigenin		 7.31±1.81
ab A		 8.41±1.54
a A		 5.51±0.80
abc AB		 5.81±0.94
ab AB		 4.23±1.00
bcd AB		 2.47±0.61
cd B		 2.24±1.03
c B
芒柄花素
Formononetin		 7.83±3.03
ab AB		 10.71±1.53
a A		 10.71±1.25
a A		 6.67±0.59
ab ABC		 5.84±0.43
b ABC		 4.21±0.48
bc BC		 1.44±0.71
c C
白杨素
Chrysin		 1.48±0.53
cd BC		 4.41±0.60
a A		 2.90±0.30
b B		 1.58±0.18
c BC		 1.98±0.30
bc B		 1.76±0.23
c BC		 0.42±0.32
d C

Fig. 2

Measured curves of seven compounds content during grain development A-G: naringenin chalcone, troxerutin, dihydroquercetin, luteolin, apigenin, formononetin, and chrysin. T1-T7: on the 5th, 10th, 15th, 20th, 25th, 30th day after anthesis and at mature stage, respectively."

[1] 诸姮, 胡宏友, 卢昌义, 李雄. 植物体内的黄酮类化合物代谢及其调控研究进展. 厦门大学学报(自然科学版), 2007,46:136-143.
Zhu H, Hu H Y, Lu C Y, Li X. Progresses on flavonoid metabolism in plants and its regulation. J Univ Xiamen (Nat Sci), 2007,46:136-143 (in Chinese with English abstract).
[2] 杨涛, 曾亚文, 萧凤回, 普晓英, 杜娟, 杨树明. 药用大麦及其活性物质研究进展. 麦类作物学报, 2007,27:1154-1158.
Yang T, Zeng Y W, Xiao F H, Pu X Y, Du J, Yang S M. Research progress on medicinal barley and its active substance. J Triticeae Crops, 2007,27:1154-1158 (in Chinese with English abstract).
[3] 周明, 沈勇根, 朱丽琴, 艾啸威, 曾璟, 杨洪烨. 植物黄酮化合物生物合成、积累及调控的研究进展. 食品研究与开发, 2016,37(18):216-221.
Zhou M, Shen Y G, Zhu L Q, Ai X W, Zeng J, Yang H Y. Research progress on biosynthesis, accumulation and regulation of flavonoids in plants. Food Res Dev, 2016,37(18):216-221 (in Chinese with English abstract).
[4] 胡少伟, 钟昆芮, 杨佳颖, 苏磊, 姜艳艳, 刘斌. 薄荷茎、叶中黄酮类成分在不同采收期的含量差异性研究. 中国中药杂志, 2018,43:544-550.
Hu S W, Zhong K R, Yang J Y, Su L, Jiang Y Y, Liu B. Study on difference of flavonoids content in stems and leaves of Mentha Haplocalycis Herba in different harvest periods. China J Chin Mat Med, 2018,43:544-550 (in Chinese with English abstract).
[5] 代吉瑞, 魏文峰, 霍金海, 王伟明. 不同采收期刺五加叶黄酮类成分动态变化分析. 中国农学通报, 2019,35(12):151-156.
Dai J R, Wei W F, Huo J H, Wang W M. Dynamic analysis of flavonoids in leaves of Acanthopanax senticosus in different harvest periods. Chin Agric Sci Bull, 2019,35(12):151-156 (in Chinese with English abstract).
[6] 王帅, 贾琦珍, 陈根元, 张玲, 马春晖. 不同生长时期小花棘豆总黄酮含量及其抗氧化活性研究. 家畜生态学报, 2014,35(8):61-65.
Wang S, Jia Q Z, Chen G Y, Zhang L, Ma C H. Total flavonoids of Oxytropis glabra dc at different growth phases and their anti-oxidative ability. J Domest Anim Ecol, 2014,35(8):61-65 (in Chinese with English abstract).
[7] 马英丽, 赵桦, 闫蕊, 王霞. 蜜楝叶、花及果实中总黄酮含量积累动态研究. 天然产物研究与开发, 2016,28:1572-1575.
Ma Y L, Zhao H, Yan R, Wang X. Accumulation of total flavonoids in different parts of Evodia lenticellata Huang. Nat Prod Res Dev, 2016,28:1572-1575 (in Chinese with English abstract).
[8] 华为, 朱靖环, 尚毅, 贾巧君, 汪军妹, 杨建明. 有色大麦研究进展. 植物遗传资源学报, 2013,14:2024-1026.
Hua W, Zhu J H, Shang Y, Jia Q J, Wang J M, Yang J M. Research advances in colored barley. J Plant Genet Resour, 2013,14:2024-1026 (in Chinese with English abstract).
[9] Bellido G G, Beta T. Anthocyanin composition and oxygen radical scavenging capacity (ORAC) of mill ed and pearled purple, black, and common barley. J Agric Food Chem, 2009,57:1022-1028.
doi: 10.1021/jf802846x
[10] Kim M J, Hyun J N, Kim J A, Park J C, Kim M Y, Kim J G, Lee S J, Chun S C, Chung I M. Relationship between phenolic compounds, anthocyanins content and antioxidant activity in colored barley germplasm. J Agric Food Chem, 2007,55:4802-4809.
doi: 10.1021/jf0701943
[11] 胡家坤, 谢文英, 陈升位, 王楠, 杨映宝, 王乐. 25个大麦地方品种籽粒总黄酮和花色苷的含量差异. 云南农业大学学报(自然科学版), 2015,30:522-527.
Hu J K, Xie W Y, Chen S W, Wang N, Yang Y B, Wang L. The content difference of total flavonoids and anthocyn in from the grains of 25 local barley varieties. J Agric Univ Yunnan (Nat Sci Edn), 2015,30:522-527 (in Chinese with English abstract).
[12] 曾亚文, 杨涛, 普晓英, 杜娟, 杨树明, 张晓燕. 大麦籽粒中γ-氨基丁酸、总黄酮和生物碱含量在发芽过程中的变化. 麦类作物学报, 2012,32:135-139.
Zeng Y W, Yang T, Pu X Y, Du J, Yang S M, Zhang X Y. Transformation of γ-aminobutyric acid and total flavones and alkaloids content in barley grains during germination process. J Triticeae Crops, 2012,32:135-139 (in Chinese with English abstract).
[13] 谢文英, 陈升位, 王楠, 王家曦, 杨映宝, 王乐. 25个地方大麦籽粒黄酮类化合物及其含量的差异分析. 云南农业大学学报(自然科学), 2015,30:657-664.
Xie W Y, Chen S W, Wang N, Wang J X, Yang Y B, Wang L. The difference analysis of the grain flavonoid components and their contents of 25 local barley varieties. J Agric Univ Yunnan (Nat Sci), 2015,30:657-664 (in Chinese with English abstract).
[14] 王楠, 王家曦, 谢文英, 陈升位, 杨映宝, 王乐. 25个地方大麦品种籽粒中花色苷成分及其含量差异. 云南农业大学学报(自然科学版), 2015,30:829-835.
Wang N, Wang J X, Xie W Y, Chen S W, Yang Y B, Wang L. Difference of the anthocyanin components and their contents of 25 local barley variety grains. J Agric Univ Yunnan (Nat Sci Edn), 2015,30:829-835 (in Chinese with English abstract).
[15] Kohyama N, Ono H, Yanagisawa T. Changes in anthocyanins in the grains of purple waxy hull-less barley during seed maturation and after harvest. J Agric Food Chem, 2008,56:5770-5774.
doi: 10.1021/jf800626b
[16] 杨晓梦, 李霞, 普晓英, 杜娟, Muhammad K A, 杨加珍, 曾亚文, 杨涛. 大麦重组自交系群体籽粒总花色苷含量和千粒重QTL定位. 作物学报, 2020,46:52-61.
Yang X M, Li X, Pu X Y, Du J, Muhammad K A, Yang J Z, Zeng Y W, Yang T. QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population. Acta Agron Sin, 2020,46:52-61 (in Chinese with English abstract).
[17] Zhang X W, Jiang Q T, Wei Y M. Inheritance analysis and mapping of quantitative trait loci (QTL) controlling individual anthocyanin compounds in purple barley (Hordeum vulgare L.) grains. PLoS One, 2017,12:e0183704.
doi: 10.1371/journal.pone.0183704
[18] 杨晓梦, 曾亚文, 普晓英, 杨涛, 杨树明, 杜娟. 大麦籽粒功能成分含量的遗传效应分析. 麦类作物学报, 2016,33:635-639.
Yang X M, Zeng Y W, Pu X Y, Yang T, Yang S M, Du J. Inheritance analysis of functional components in barley grains. J Triticeae Crops, 2016,33:635-639 (in Chinese with English abstract).
[19] 沈真辉, 王楠, 李静烨, 王家曦, 陈升位, 李应秋, 吴海燕, 王卫斌, 陈柚汐. 25份大麦材料成熟籽粒中黄酮醇化合物含量的差异分析和QTLs检测. 分子植物育种, 2016,14:2197-2204.
Shen Z H, Wang N, Li J Y, Wang J X, Chen S W, Li Y Q, Wu H Y, Wang W B, Chen Y X. The variance analysis and the QTLs detection on the content of flavonol compounds from the grains of 25 barley materials. Mol Plant Breed, 2016,14:2197-2204 (in Chinese with English abstract).
[20] Abdel-Aal E S M, Young J C, Rabalski I. Anthocyanin composition in black, blue, pink, purple, and red cereal grains. J Agric Food Chem, 2006,54:4696-4704.
doi: 10.1021/jf0606609
[21] 余春磊, 齐国昌, 张鹍飞, 袁金娥, 罗小娇, 冯宗云. 大麦花后四种主要黄酮物质含量的动态变化. 麦类作物学报, 2016,34:222-226.
Yu C L, Qi G C, Zhang K F, Yuan J E, Luo X J, Feng Z Y. Studies on the accumulation of four major flavonoids after flowering in barley. J Triticeae Crops, 2016,34:222-226 (in Chinese with English abstract).
[22] 张鹍飞, 余春磊, 齐国昌, 袁金娥, 刘新春, 冯宗云. 大麦籽粒不同部位黄酮含量的比较分析. 麦类作物学报, 2014,34:1441-1446.
Zhang K F, Yu C L, Qi G C, Yuan J E, Liu X C, Feng Z Y. Comparative analysis on the flavonoids content in different parts of barley grains. J Triticeae Crops, 2014,34:1441-1446 (in Chinese with English abstract).
[23] 王芳, 王宪泽. 小麦籽粒淀粉合成动态及其相关酶活性的研究. 麦类作物学报, 2004,24:57-60.
Wang F, Wang X Z. Study on the dynamic changes of starch synthesis and their related enzymes in wheat. J Triticeae Crops, 2004,24:57-60 (in Chinese with English abstract).
[24] 谭彩霞, 封超年, 郭文善, 朱新开, 李春燕, 彭永欣. 花后不同时期高温处理下小麦籽粒的淀粉合成及相关酶基因表达. 核农学报, 2012,26:1311-1316.
Tan C X, Feng C N, Guo W S, Zhu X K, Li C Y, Peng Y X. Effect of high temperature during different growth stage after anthesis on starch synthesis and related enzyme gene expression in wheat grains. J Nucl Agric Sci, 2012,26:1311-1316 (in Chinese with English abstract).
[25] 罗小娇, 刘新春, 杨晓云, 袁金娥, 冯宗云. 青稞C4H基因的克隆及组织表达分析. 植物遗传资源学报, 2014,15:589-596.
Luo X J, Liu X C, Yang X Y, Yuan J E, Feng Z Y. Cloning and tissue expression analysis of HvC4H gene in Hulless barley. J Plant Genet Resour, 2014,15:589-596 (in Chinese with English abstract).
[26] 杨晓云, 杨智敏, 罗小娇, 孔德媛, 袁金娥, 刘新春, 冯宗云. 青稞4-香豆酸辅酶A连接酶基因4CL的克隆及表达分析. 麦类作物学报, 2014,34:1603-1610.
Yang X Y, Yang Z M, Luo X J, Kong D Y, Yuan J E, Liu X C, Feng Z Y. Cloning and expression analysis of 4-ceoumarate: CoA ligase gene 4CL in hulless barley. J Triticeae Crops, 2014,34:1603-1610 (in Chinese with English abstract).
[1] HUANG Qi-Xiu,QU Yan-Ying,YAO Zheng-Pei,LI Meng-Yu,CHEN Quan-Jia*. Correlation between Resistance to Fusarium Wilt and Expression of Flavonoid Metabolism Related Genes in Gossypium barbadense L. [J]. Acta Agron Sin, 2017, 43(12): 1791-1801.
[2] ZHAO Xin-Hua, SHU Hong-Mei, WANG You-Hua, CHEN Bing-Lin, ZHOU Zhi-Guo. Effects of Sowing Date on Accumulation and Distribution of Biomass and Nitrogen in Cotton Bolls [J]. Acta Agron Sin, 2010, 36(10): 1707-1714.
[3] ZHONG Xiu-Li;WANG Dao-Long;YOSHIDA Hisashi;HU Xin;ZHAO Peng;HAN Li-Shuai;WANG Xiao-Guang;HUANG Shao-Hua;HUANG Jian-Ying;SUN Zhong-Fu. Analysis on the Factors Affecting Frost Resistance for Winter Wheat [J]. Acta Agron Sin, 2007, 33(11): 1810-1814.
[4] ZONG Xue-Feng; ZHANG Jian-Kui;LI Bang-Xiu; YU Guo-Dong;SHI You-Ming and WANG San-Gen. Relationship between Antioxidation and Grain Colors of Wheat (Triticum aestivum L.) [J]. Acta Agron Sin, 2006, 32(02): 237-242.
[5] ZHAO Xiang-Qian; WANG Xue-De. Composition Analysis of Pigment in Colored Cotton Fiber [J]. Acta Agron Sin, 2005, 31(04): 456-462.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd