Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (12): 3045-3056.doi: 10.3724/SP.J.1006.2022.12080

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development of functional markers and genotype screening for nitrogen use efficiency genes in rice

TAO Ya-Jun1,3(), ZHU Jing-Yan2, WANG Jun1,3, FAN Fang-Jun1,3, XU Yang1,3, LI Wen-Qi1,3, WANG Fang-Quan1,3, CHEN Zhi-Hui1,3, JIANG Yan-Jie1,3, ZHU Jian-Ping1,3, LI Xia1,3, YANG Jie1,3()   

  1. 1Jiangsu High Quality Rice Center / Nanjing Branch of Chinese National Center for Rice Improvement / Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
    2College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China
    3Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops / Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Received:2021-11-29 Accepted:2022-03-25 Online:2022-12-12 Published:2022-04-20
  • Contact: YANG Jie E-mail:ricetao@163.com;yangjie168@aliyun.com
  • Supported by:
    Jiangsu Province Key Research and Development Program Modern Agriculture(BE2020339);Natural Science Foundation of Jiangsu Province(BK20190255);National Natural Science Foundation of China(31901525);Jiangsu Province Key Research and Development Program Modern Agriculture(BE2021374);International Cooperation and Exchange of the National Natural Science Foundation of China(31861143011);Jiangsu Agricultural Science and Technology Innovation Fund(CX(19)1002)

RichHTML405

46

PDF

3270

Abstract:

Nitrogen is an essential mineral element that affects plants biomass and yield formation, and its efficient and reasonable utilization is an important guarantee for sustainable agricultural. Breeding rice varieties containing high nitrogen use efficiency (NUE) genes is an effective way to increase NUE and reduce the amount of nitrogen fertilizer. In this study, five genes, OsNR2, OsNPF6.1, OsTCP19, OsLHT1, and OsGRF4, were selected from the aspects of nitrogen absorption, transportation, and assimilation. Based on the reported functional haplotype, co-segregated markers were designed. Using six pairs of allele-specific PCR (AS-PCR) markers and one pair of InDel marker, 70 indica rice, 34 japonica rice, and 84 Taihu rice resources were identified. The results showed that OsNR2 was widely distributed in indica rice, while OsNPF6.1, OsTCP19, and OsGRF4 were less distributed. All 34 japonica rice and 84 Taihu rice resources only contained OsLHT1. We also successfully obtained two indica germplasms, which contained OsNR2, OsNPF6.1, and OsGRF4. The functional markers developed in this research and two materials provide technical support for breeding new rice varieties with high NUE through molecular marker-assisted selection (MAS) methods.

Key words: rice, high nitrogen-use efficiency, AS-PCR, InDel markers

Table 1

Functional marker primers designed based mutation sites"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')		 PCR片段大小
PCR fragment (bp)
NR2-F AGGGCGAGGACCCCAAGT
NR2-R GTCGTCGGGCTGGTCCCA 242
nr2-R GTCGTCGGGCTGGTCCCT 242
NPF6.1-F ATTATGGAACGGAGGGAG
NPF6.1-R CCCGGATGTCCACAACAC 246
npf6.1-R CCCGGATGTCCACAACAT 246
TCP19-INDEL-F TAACTCTTCAGGGTTCTTGC
TCP19-INDEL-R TGTGCCGTGTCACATAGA 253
LHT1-1-F GTTGCGGCTGACAATAAA
LHT1-1-R AGCATGGGGTCAAAGCAA 317
lht1-1-R AGCATGGGGTCAAAGCAG 317
LHT1-2-F TCATAATGCTTACCCTCT
LHT1-2-R TGGAGGGTTGACTCGTAA 166
lht1-2-R TGGAGGGTTGACTCGTAT 166
GRF4-1-F AACCAAATAAGCCCTTCA
GRF4-1-R ATGTGATAAAAAAGCTGTAA 329
grf4-1-R ATGTGATAAAAAAGCTGTAT 329
GRF4-2-F AACCAAATAAGCCCTTCA
GRF4-2-R TAGTTCACGCTAAAATTAG 410
grf4-2-R TAGTTCACGCTAAAATTAA 410

Fig. 1

Functional classification and variations of NUE genes in rice A: the functional classification of NUE genes; B: the variations of NUE genes."

Fig. 2

PCR amplification of different functional markers Lane 1, 3: NR2 marker; Lane 2, 4: nr2 marker; Lane 5, 7: NPF6.1 marker; Lane 6, 8: npf6.1 marker; Lane 9, 11: LHT1-1 marker; Lane 10, 12: lht1-1 marker; Lane 13, 15: LHT1-2 marker; Lane 14, 16: lht1-2 marker; Lane 17, 19: GRF4-1 marker; Lane 18, 20: grf4-1 marker; Lane 21, 23: GRF4-2 marker; Lane 22, 24: grf4-2 marker; Lane 25: PCR amplification of ‘Nipponbare’ using TCP19-INDEL marker; Lane 26: PCR amplification of ‘Kasalath’ using TCP19-InDel marker. M: DL2000 marker."

Table 2

Amplification parents and sites of designed functional markers"

标记名称
Marker name		 扩增亲本
Amplification parent		 扩增位点
Amplification site
NR2 NIP OsNR2779T
nr2 9311 OsNR2779A
NPF6.1 NIP OsNPF6.1160G
npf6.1 NJ11 OsNPF6.1160A
TCP19 NIP, Kasalath OsTCP启动子区29 bp的InDel A 29 bp InDel in the OsTCP19 promoter
LHT1-1 NIP OsLHT1-2140T
lht1-1 9311 OsLHT1-2140C
LHT1-2 NIP OsLHT1-595T
lht1-2 9311 OsLHT1-595A
GRF4-1 NIP OsGRF4-884T
grf4-1 RD23 OsGRF4-884A
GRF4-2 NIP OsGRF4-801C
grf4-2 RD23 OsGRF4-801T

Table 3

Distribution of NUE genes in indica"

材料Material OsNR2 OsNPF6.1 OsTCP19 OsLHT1-1 OsLHT1-2 OsGRF4-1 OsGRF4-2
951227 1 1 0 0 0 1 1
南京16 Nanjing 16 1 1 0 0 0 1 1
Z03108 1 1 0 0 0 0 0
04-286 1 1 0 0 0 0 0
扬稻8号 Yangdao 8 1 1 0 0 0 0 0
扬州98127 Yangzhou 98127 1 1 0 0 0 0 0
9311 1 1 0 0 0 0 0
中佳2号 Zhongjia 2 1 1 0 0 0 0 0
苏农3004 Sunong 3004 1 1 0 0 0 0 0
扬籼9850 Yangxian 9850 1 1 0 0 0 0 0
R6547 1 1 0 0 0 0 0
R6548 1 1 0 0 0 0 0
镇籼886 Zhenxian 886 1 0 0 0 0 0 0
IRRI超高产系 IRRI super high-yield line 1 0 0 0 0 0 0
金桂占选 Jinguizhanxuan 1 0 0 0 0 1 1
广超丝苗 Guangchaosimiao 1 0 0 0 0 0 0
玉香油占 Yuxiangyouzhan 1 0 0 0 0 0 0
银花占2号 Yinhuazhan 2 1 0 0 0 0 0 0
丰华占 Fenghuazhan 1 0 0 0 0 0 0
茉莉软占 Moliruanzhan 1 0 0 0 0 0 0
黄华占 Huanghuazhan 1 0 0 0 0 0 0
五山油占 Wushanyouzhan 1 0 1 0 0 0 0
茉莉丝占 Molisizhan 1 0 0 0 0 0 0
矮秀占 Aixiuzhan 1 0 0 0 0 0 0
茉莉油占 Moliyouzhan 1 0 0 0 0 0 0
丰新占 Fengxinzhan 1 0 1 0 0 0 0
黄美占 Huangmeizhan 1 0 0 0 0 0 0
黄莉占 Huanglizhan 1 0 0 0 0 0 0
黄丝占 Huangsizhan 1 0 0 0 0 0 0
丰二占 Feng’erzhan 1 0 0 0 0 0 0
华新占 Huaxinzhan 1 0 0 0 0 0 0
新514 Xin 514 1 1 0 0 0 0 0
R9912 1 0 0 0 0 1 1
浙恢7954 Zhehui 7954 1 0 0 0 0 0 0
泸恢17 Luhui 17 1 1 0 0 0 0 0
蜀恢881 Shuhui 881 1 0 0 0 0 0 0
恢76 Hui76 1 0 0 0 0 0 0
辐恢718 Fuhui 718 1 0 0 0 0 0 0
内香恢1号 Neixianghui 1 1 1 0 0 0 0 0
内多恢1号 Neiduohui 1 1 1 0 0 0 0 0
盐恢559 Yanhui 559 1 0 0 0 0 0 0
川恢725 Chuanhui 725 1 0 0 0 0 0 0
广恢128 Guanghui 128 1 0 0 0 0 0 0
湘747 Xiang 747 1 0 0 0 0 0 0
丰籼1号 Fengxian 1 1 0 0 0 0 0 0
小农占 Xiaonongzhan 1 0 0 0 0 0 0
五山丝苗 Wushansimiao 1 0 0 0 0 0 0
黄花占 Huanghuazhan 1 0 0 0 0 0 0
粤香占 Yuexiangzhan 1 0 0 0 0 0 0
粤禾丝苗 Yuehesimiao 1 1 0 0 0 0 0
粤综占 Yuezongzhan 1 1 0 0 0 0 0
粤农丝苗 Yuenongsimiao 1 1 0 0 0 0 0
金农丝苗 Jinnongsimiao 1 0 0 0 0 0 0
宁317 Ning 317 1 0 0 0 0 0 0
南农3017 Nannong 3017 1 1 0 0 0 0 0
泸恢195 Luhui 195 1 0 0 0 0 0 0
泸恢17 Luhui 17 1 0 0 0 0 0 0
桂99 Gui 99 1 0 0 0 0 0 0
辐恢838 Fuhui 838 1 0 0 0 0 0 0
Basmati-1 1 0 0 0 0 0 0
成恢149 Chenghui 149 1 0 0 0 0 0 0
绵恢725 Mianhui 725 1 1 0 0 0 0 0
E32辐照选-15 E32 radiation-15 1 1 0 0 0 0 0
成恢22 Chenghui 22 1 1 0 0 0 0 0
中香11 Zhongxiang 11 1 0 0 0 0 1 1
L30012 1 1 0 0 0 0 0
新513 Xin 513 1 0 0 0 0 0 0
胜泰1号 Shengtai 1 1 0 0 0 0 0 0
L30015 1 0 0 0 0 0 0
Kasalath 1 1 0 0 0 0 0

Table 4

Distribution of NUE genes in japonica"

材料Material OsNR2 OsNPF6.1 OsTCP19 OsLHT1-1 OsLHT1-2 OsGRF4-1 OsGRF4-2
连粳7号 Lianjing 7 0 0 0 1 1 0 0
连粳10号 Lianjing 10 0 0 0 1 1 0 0
徐稻3号 Xudao 3 0 0 0 1 1 0 0
淮稻5号 Huaidao 5 0 0 0 0 0 0 0
盐稻8号 Yandao 8 0 0 0 0 0 0 0
扬育粳2号 Yangyujing 2 0 0 0 1 1 0 0
扬9709 Yang 9709 0 0 0 1 1 0 0
扬中稻1号 Yangzhongdao 1 0 0 0 1 1 0 0
武陵粳1号 Wulingjing 1 0 0 0 1 1 0 0
扬辐粳1号 Yangfujing 1 0 0 0 1 1 0 0
宁粳1号 Ningjing 1 0 0 0 1 1 0 0
南粳49 Nanjing 49 0 0 0 1 1 0 0
南粳46 Nanjing 46 0 0 0 1 1 0 0
南粳5055 Nanjing 5055 0 0 0 1 1 0 0
苏沪香粳 Suhuxiangjing 0 0 0 1 1 0 0
武香粳14号 Wuxiangjing 14 0 0 0 1 1 0 0
武运粳7号 Wuyunjing 7 0 0 0 1 1 0 0
武运粳23号 Wuyunjing 23 0 0 0 1 1 0 0
苏香粳2号 Suxiangjing 2 0 0 0 1 1 0 0
嘉33 Jia 33 0 0 0 1 1 0 0
嘉991 Jia 991 0 0 0 1 1 0 0
镇稻1号 Zhendao 1 0 0 0 1 1 0 0
日辉粳6号 Rihuijing 6 0 0 0 0 0 0 0
泗阳紫稻 Siyangzidao 0 0 0 1 1 0 0
楚粳39 Chujing 39 0 0 0 0 0 0 0
云光104 Yunguang 104 0 0 0 1 1 0 0
粳优165 Jingyou 165 0 0 0 1 1 0 0
小粒香 Xiaolixiang 0 0 0 1 1 0 0
越光BL Yueguang BL 0 0 0 1 1 0 0
苏秀867 Suxiu 867 0 0 0 1 1 0 0
苏垦118 Suken 118 0 0 0 0 0 0 0
东北194 Dongbei 194 0 0 0 1 1 0 0
临稻16 Lindao 16 0 0 0 1 1 0 0
降糖稻 Jiangtangdao 0 0 0 0 0 0 0

Table 5

Distribution of NUE genes in Taihu resources"

材料Material NR2 NPF6.1 TCP19 LHT1-1 LHT1-2 GRF4-1 GRF4-2
抱芯太湖青 Baoxintaihuqing 0 0 0 1 1 0 0
矮脚太湖青 Aijiaotaihuqing 0 0 0 1 1 0 0
上海青 Shanghaiqing 0 0 0 1 1 0 0
海冬青 Haidongqing 0 0 0 1 1 0 0
常梗青 Changenqing 0 0 0 1 1 0 0
立更青 Ligengqing 0 0 0 1 1 0 0
齐江青 Qijiangqing 0 0 0 1 1 0 0
矮种罗汉黄 Aizhongluohanhuang 0 0 0 1 1 0 0
呆长青 Daichangqing 0 0 0 1 1 0 0
万年青 Wannianqing 0 0 0 1 1 0 0
韭菜青 Jiucaiqing 0 0 0 1 1 0 0
茭白叶青 Jiaobaiyeqing 0 0 0 1 1 0 0
大红稻(老来红) Dahongdao 0 0 0 1 1 0 0
老头老来红 Laotoulaolaihong 0 0 0 1 1 0 0
早黑头红 Zaoheitouhong 0 0 0 1 1 0 0
晚罗汉稻 Wanluohandao 0 0 0 1 1 0 0
晚牛毛黄 Wanniumaohuang 0 0 0 1 1 0 0
TH93 0 0 0 1 1 0 0
白石稻 Baishidao 0 0 0 1 1 0 0
大量稻 Daliangdao 0 0 0 1 1 0 0
白薄稻 Baibodao 0 0 0 1 1 0 0
二黑稻 Eeheidao 0 0 0 1 1 0 0
摧稻 Cuidao 0 0 0 1 1 0 0
红虹稻 Hongxiadao 0 0 0 1 1 0 0
鹅营白粳稻 Eyingbaijingdao 0 0 0 1 1 0 0
大穗头粳稻 Dasuitoujingdao 0 0 0 1 1 0 0
矮子粳稻 Aizijingdao 0 0 0 1 1 0 0
长黄稻 Changhuangdao 0 0 0 1 1 0 0
矮黄稻 Aihuangdao 0 0 0 1 1 0 0
减晚慢种 Jianwanmanzhong 0 0 0 1 1 0 0
天下第一种 Tianxiadiyizhong 0 0 0 1 1 0 0
红芒种 Hongmangzhong 0 0 0 1 1 0 0
矮洁种 Aijiezhong 0 0 0 1 1 0 0
瓜田种 Guatianzhong 0 0 0 1 1 0 0
龙沟种 Longgouzhong 0 0 0 1 1 0 0
老大种 Laodazhong 0 0 0 1 1 0 0
老虎种 Laohuzhong 0 0 0 1 1 0 0
余山种 Yushanzhong 0 0 0 1 1 0 0
TH262 0 0 0 1 1 0 0
江北种 Jiangbeizhong 0 0 0 1 1 0 0
TH274 0 0 0 1 1 0 0
TH275 0 0 0 1 1 0 0
白稻头 Baidaotou 0 0 0 1 1 0 0
TH284 0 0 0 1 1 0 0
TH301 0 0 0 1 1 0 0
TH303 0 0 0 1 1 0 0
TH324 0 0 0 1 1 0 0
TH345 0 0 0 1 1 0 0
TH346 0 0 0 1 1 0 0
TH359 0 0 0 1 1 0 0
不留名 Buliuming 0 0 0 1 1 0 0
TH369 0 0 0 1 1 0 0
甩杀极 Shuaishaji 0 0 0 1 1 0 0
TH373 0 0 0 1 1 0 0
TH374 0 0 0 1 1 0 0
TH376 0 0 0 1 1 0 0
TH380 0 0 0 1 1 0 0
TH382 0 0 0 1 1 0 0
TH384 0 0 0 1 1 0 0
TH385 0 0 0 1 1 0 0
南束罗汉黄 Nanshuluohanhuang 0 0 0 1 1 0 0
润叶黄 Runyehuang 0 0 0 1 1 0 0
TH390 0 0 0 1 1 0 0
有芒旱稻 Youmanghandao 0 0 0 1 1 0 0
TH394 0 0 0 1 1 0 0
TH403 0 0 0 1 1 0 0
TH407 0 0 0 1 1 0 0
TH413 0 0 0 1 1 0 0
六十子粳 Liushizijing 0 0 0 1 1 0 0
东方红1号(穗1) Dongfanghong 1 0 0 0 1 1 0 0
小红早 Xiaohongzao 0 0 0 1 1 0 0
润叶黄 Runyehuang 0 0 0 1 1 0 0
早小白稻 Zaoxiaobaidao 0 0 0 1 1 0 0
抖山旱稻(陆稻) Doushanhandao 0 0 0 1 1 0 0
早飞来风 Zaofeilaifeng 0 0 0 1 1 0 0
一时兴 Yishixing 0 0 0 1 1 0 0
头等一时兴 Toudengyishixing 0 0 0 1 1 0 0
香粳糯稻 Xiangjingnuodao 0 0 0 1 1 0 0
TH433 0 0 0 1 1 0 0
TH434 0 0 0 1 1 0 0
TH436 0 0 0 1 1 0 0
红芒糯 Hongmangnuo 0 0 0 1 1 0 0
TH438 0 0 0 1 1 0 0
TH444 0 0 0 1 1 0 0

Table 6

Sequencing primers for NUE genes designed and SNP (InDel) analysis"

引物名称 Primer name 引物序列 Primer sequence (5'-3')
NR2-CX F: AGGGCGAGGACCCCAAGT; R: TGTCGAGGTCATAGCCCATCTT
NPF6.1-CX F: ATTATGGAACGGAGGGAG; R: GGAGATAGAGGGTGGTGAA
TCP19-CX F: TAACTCTTCAGGGTTCTTGC; R: TGTGCCGTGTCACATAGA
LHT1-1-CX F: GTTGCGGCTGACAATAAA; R: AATCTCGTGGGAAATCTG
LHT1-2-CX F: TCATAATGCTTACCCTCT; R: TTGTTTTGCTAACTCACA
GRF4-CX F: AACCAAATAAGCCCTTCA; R: TTAGTCTGCTGCTCCAAC

Fig. 3

Sequence alignment of functional domains for partial rice varieties A:the partial sequence of OsNR2 in the 9311; B: the partial sequence of OsNPF6.1 in R6547; C: the partial sequence of OsLHT1-1 in SK118; D: the partial sequence of OsLHT1-2 in Huaidao 5; E: the partial sequence of OsGRF4-1 in Jinguizhanxuan; F: the partial sequence of OsGRF4-2 in Nanjing 16; G: the partial sequence of OsTCP19 in Wushanyouzhan. Red box indicates different SNP or InDel."

[1] 张超普, 余四斌, 张启发. 绿色超级稻新品种选育研究进展. 生命科学, 2018, 30: 1083-1089.
Zhang C P, Yu S B, Zhang Q F. Recent advances in green super rice development. Chin Sci Bull, 2018, 30: 1083-1089. (in Chinese with English abstract)
[2] Hu B, Wang W, Ou S, Tang J, Li H, Che R, Zhang Z, Chai X, Wang H, Wang Y, Liang C, Liu L, Piao Z, Deng Q, Deng K, Xu C, Liang Y, Zhang L, Li L, Chu C. Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies. Nat Genet, 2015, 47: 834-838.
doi: 10.1038/ng.3337
[3] Liu Y, Wang H, Jiang Z, Wang W, Xu R, Wang Q, Zhang Z, Li A, Liang Y, Ou S, Liu X, Cao S, Tong H, Wang Y, Zhou F, Liao H, Hu B, Chu C. Genomic basis of geographical adaptation to soil nitrogen in rice. Nature, 2021, 590: 600-605.
doi: 10.1038/s41586-020-03091-w
[4] Gao Z, Wang Y, Chen G, Zhang A, Yang S, Shang L, Wang D, Ruan B, Liu C, Jiang H, Dong G, Zhu L, Hu J, Zhang G, Zeng D, Guo L, Xu G, Teng S, Harberd N P, Qian Q. The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency. Nat Commun, 2019, 10: 5207.
doi: 10.1038/s41467-019-13110-8
[5] Yan M, Fan X, Feng H, Miller A J, Shen Q, Xu G. Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges. Plant Cell Environ, 2011, 34: 1360-1372.
doi: 10.1111/j.1365-3040.2011.02335.x
[6] Xia X, Fan X, Wei J, Feng H, Qu H, Xie D, Miller A J, Xu G. Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport. J Exp Bot, 2015, 66: 317-331.
doi: 10.1093/jxb/eru425 pmid: 25332358
[7] Guo N, Hu J, Yan M, Qu H, Luo L, Tegeder M, Xu G. Oryza sativa Lysine-Histidine-type Transporter 1 functions in root uptake and root-to-shoot allocation of amino acids in rice. Plant J, 2020, 103: 395-411.
doi: 10.1111/tpj.14742
[8] Li S, Tian Y, Wu K, Ye Y, Yu J, Zhang J, Liu Q, Hu M, Li H, Tong Y, Harberd N P, Fu X. Modulating plant growth-metabolism coordination for sustainable agriculture. Nature, 2018, 560: 595-600.
doi: 10.1038/s41586-018-0415-5
[9] Tang W, Ye J, Yao X, Zhao P, Xuan W, Tian Y, Zhang Y, Xu S, An H, Chen G, Yu J, Wu W, Ge Y, Liu X, Li J, Zhang H, Zhao Y, Yang B, Jiang X, Peng C, Zhou C, Terzaghi W, Wang C, Wan J. Genome-wide associated study identifies NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice. Nat Commun, 2019, 10: 5279.
doi: 10.1038/s41467-019-13187-1 pmid: 31754193
[10] Xu G, Fan X, Miller A. Plant nitrogen assimilation and use efficiency. Annu Rev Plant Biol, 2012, 63: 153-182.
doi: 10.1146/annurev-arplant-042811-105532 pmid: 22224450
[11] Andersen J R, Lubberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8: 554-560.
pmid: 14607101
[12] 方琳, 陶亚军, 张灵, 范方军, 张爱伟, 李文奇, 王芳权, 许扬, 陈智慧, 蒋彦婕, 杨杰, 王军. 水稻氮高效基因NRT1.1B功能标记开发和资源筛选. 分子植物育种, 2020, 18: 7795-7800.
Fang L, Tao Y J, Zhang L, Fan F J, Zhang A W, Li W Q, Wang F Q, Xu Y, Jiang Y J, Yang J, Wang J. Development of functional marker and screening resources for high nitrogen use efficiency gene NRT1.1B in rice. Mol Plant Breed, 2020, 18: 7795-7800. (in Chinese with English abstract)
[13] 王军, 杨杰, 陈志德, 仲维功. 稻香米基因标记的开发与应用. 分子植物育种, 2008, 6: 1209-1212.
Wang J, Yang J, Chen Z D, Zhong W G. Development and application of fragrance gene markers in rice. Mol Plant Breed, 2008, 6: 1209-1212. (in Chinese with English abstract)
[14] 王军, 赵婕宇, 许扬, 范方军, 朱金燕, 李文奇, 王芳权, 费云燕, 仲维功, 杨杰. 水稻稻瘟病抗性基因Bsr-d1功能标记的开发和利用. 作物学报, 2018, 44: 1612-1620.
Wang J, Zhao J Y, Xu Y, Fan F J, Zhu J Y, Li W Q, Wang F Q, Fei Y Y, Zhong W G, Yang J. development and application of functional markers for rice blast resistance gene Bsr-d1 in rice. Acta Agron Sin, 2018, 44: 1612-1620. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2018.01612
[15] 王芳权, 陈智慧, 许扬, 王军, 李文奇, 范方军, 陈丽琴, 陶亚军, 仲维功, 杨杰. 水稻广谱抗稻瘟病基因PigmR功能标记的开发及应用. 中国农业科学, 2019, 52: 955-967.
Wang F Q, Chen Z H, Xu Y, Wang J, Li W Q, Fan F J, Chen L Q, Tao Y J, Zhong W G, Yang J. Development and application of the functional marker for the broad-spectrum blast resistance gene PigmR in rice. Sci Agric Sin, 2019, 52: 955-967. (in Chinese with English abstract)
[16] 陈智慧, 王芳权, 许扬, 王军, 李文奇, 范方军, 仲维功, 杨杰. 软米基因Wx-mp在部分粳稻品种资源中的分布. 植物遗传资源学报, 2019, 20: 975-981.
Chen Z H, Wang F Q, Xu Y, Wang J, Li W Q, Fan F J, Zhong W G, Yang J. The distribution of low amylose content allele Wx-mp in japonica rice. J Plant Genet Res, 2019, 20: 975-981. (in Chinese with English abstract)
[17] 刘立军, 王康君, 卞金龙, 熊溢伟, 陈璐, 王志琴, 杨建昌. 水稻产量对氮肥响应的品种间差异及其与根系形态生理的关系. 作物学报, 2014, 40: 1999-2007.
doi: 10.3724/SP.J.1006.2014.01999
Liu L J, Wang K J, Bian J L, Xiong Y W, Chen L, Wang Z Q, Yang J C. Differences in yield response to nitrogen fertilizer among rice cultivars andtheir relationship with root morphology and physiology. Acta Agron Sin, 2014, 40: 1999-2007 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2014.01999
[18] Song M, Fan X, Chen J, Qu H, Luo L, Xu G. OsNAR2.1 Interaction with OsNIT1 and OsNIT2 functions in root-growth responses to nitrate and ammonium. Plant Physiol, 2020, 183: 289-303.
doi: 10.1104/pp.19.01364 pmid: 32071150
[19] Huang S, Liang Z, Chen S, Sun H, Fan X, Wang C, Xu G, Zhang Y. A transcription factor, OsMADS57, regulates long-distance nitrate transport and root elongation. Plant Physiol, 2019, 180: 882-895.
doi: 10.1104/pp.19.00142
[20] Li C, Tang Z, Wei J, Qu H, Xie Y, Xu G. The OsAMT1.1 gene functions in ammonium uptake and ammonium-potassium homeostasis over low and high ammonium concentration ranges. J Genet Genomics, 2016, 43: 639-649.
doi: 10.1016/j.jgg.2016.11.001
[21] Ranathunge K, El-Kereamy A, Gidda S, Bi Y, Rothstein S. AMT1;1 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. J Exp Bot, 2014, 65: 965-979.
doi: 10.1093/jxb/ert458 pmid: 24420570
[22] Tang Z, Fan X, Li Q, Feng H, Miller A J, Shen Q, Xu G. Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx. Plant Physiol, 2012, 160: 2052-2063.
doi: 10.1104/pp.112.204461 pmid: 23093362
[23] Fan X, Tang Z, Tan Y, Zhang Y, Luo B, Yang M, Lian X, Shen Q, Miller A J, Xu G. Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields. Proc Natl Acad Sci USA, 2016, 113: 7118-7123.
doi: 10.1073/pnas.1525184113
[24] Funayama K, Kojima S, Tabuchi-Kobayashi M, Sawa Y, Nakayama Y, Hayakawa T, Yamaya T. Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots. Plant Cell Physiol, 2013, 54: 934-943.
doi: 10.1093/pcp/pct046 pmid: 23509111
[25] Ohashi M, Ishiyama K, Kojima S, Kojima M, Sakakibara H, Yamaya T, Hayakawa T. Lack of cytosolic glutamine synthetase1;2 activity reduces nitrogen-dependent biosynthesis of cytokinin required for axillary bud outgrowth in rice seedlings. Plant Cell Physiol, 2017, 58: 679-690.
doi: 10.1093/pcp/pcx022 pmid: 28186255
[26] Yang X, Nian J, Xie Q, Feng J, Zhang F, Jing H, Zhang J, Dong G, Liang Y, Peng J, Wang G, Qian Q, Zuo J. Rice Ferredoxin-dependent glutamate synthase regulates nitrogen-carbon metabolomes and is genetically differentiated between japonica and indica subspecies. Mol Plant, 2016, 9: 1520-1534.
doi: 10.1016/j.molp.2016.09.004
[27] Zeng D D, Qin R, Li M, Alamin M, Jin X L, Liu Y, Shi C H. The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice. Mol Genet Genomics, 2017, 292: 385-395.
doi: 10.1007/s00438-016-1275-z
[1] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[2] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[3] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[4] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[5] Shi Shao-Jie, Liu Kai, Chen Zi-Yi, Wang Hui-Ying, Li San-He, Zhou Lei, You Ai-Qing. Cloning and functional analysis of the dwarf and multi-tiller gene DMT1 in rice [J]. Acta Agronomica Sinica, 2026, 52(4): 1022-1034.
[6] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[7] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[8] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[9] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[10] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[11] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[12] GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng. Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive [J]. Acta Agronomica Sinica, 2025, 51(9): 2433-2453.
[13] CHEN Hui-Ying, HE Jia-Xin, ZHU Bin, HUANG Shi-Xuan, ZHOU Xing-You, WU Jun-Quan, YANG Mei-Yan. Whole genome analysis and biological characterization of phage vB_XaS_ HDB2 infected with Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2025, 51(8): 2087-2099.
[14] YANG Hai-Yang, WU Lin-Xuan, LI Bo-Wen, SHI Han-Feng, YUAN Xi-Long, LIU Jin-Zhao, CAI Hai-Rong, CHEN Shi-Yi, GUO Tao, WANG Hui. OsWRI3, identified based on QTL mapping, regulates seed shattering in rice [J]. Acta Agronomica Sinica, 2025, 51(7): 1712-1724.
[15] WANG Fen, WU Dong-Li, ZHANG Quan-Jun. Response of phenological phase stages of single-cropping rice to climate change in Hubei province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1934-1948.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd