割手密萜烯合成酶(TPS)基因家族分析及其在生物胁迫下的表达分析

doi:10.3724/SP.J.1006.2022.14237

Abstract

Abstract:

Terpenoids produced by the action of terpene synthase (TPS) enzymes play important roles in plant biotic and abiotic stress. Saccharum spontaneum, an important parent material of modern sugarcane cultivars, contains a large number of stress resistance genes. To investigate the characteristics and functions of the TPS gene family in S. spontaneum, 39 SsTPS genes were identified in S. spontaneum genome that encoded proteins with two conserved domains (PF01397 and PF03936) by using an HMMER search. The SsTPS proteins were divided into TPS-a, b, e/f, and g subfamilies. The SsTPS gene family had mainly expanded through segmental duplications, and a total of 12 SsTPS genes involved in segmental duplication events. In addition, qRT-PCR showed that the expression patterns of some SsTPS genes differed in S. spontaneum between Spodoptera frugiperda-stressed and Xanthomonas albilineans-infected plants, whereas the relative expression levels of seven SsTPS genes were strongly up-regulated. Notably, SsTPS15 were up-regulated in response to Spodoptera frugiperda-stressed but were down-regulated by X. albilineans infection, while SsTPS26, SsTPS37, and SsTPS39 had the opposite results. These results will be of great significance to further understanding the biological roles of terpene synthases and to develop resistant breeding in S. spontaneum.

Key words: Saccharum spontaneum, terpene synthase gene, biotic stresses, expression analysis, resistance breeding

LIN Huan-Tai, ZHANG Tian-Jie, SHI Meng-Ting, GUO Yan-Fang, GAO San-Ji, WANG Jin-Da. Genome-wide analysis of terpene synthase (TPS) gene family and its expression under biological stress in Saccharum spontaneum[J].Acta Agronomica Sinica, 2022, 48(12): 3029-3044.

Figures/Tables 10

Table 1

Table 2

Basin information and physiochemical properties of SsTPS genes family in S. spontaneum"

基因名称 Gene name	基因编号 Gene ID	氨基酸数 Number of amino acids	等电点 pI	分子量 Molecular weight (kD)	负电荷残基 Asp+Glu	正电荷残基Arg+Lys	不稳定系数Coefficient of instability	平均疏水性 GRAVY	脂肪系数 AI	α-螺旋 α-helix(%)	延伸链 Extended chain (%)	无规则卷曲 Random curl (%)
基因名称 Gene name	基因编号 Gene ID	氨基酸数 Number of amino acids	等电点 pI	分子量 Molecular weight (kD)	负电荷残基 Asp+Glu	正电荷残基Arg+Lys	不稳定系数Coefficient of instability	平均疏水性 GRAVY	脂肪系数 AI	α-螺旋 α-helix(%)	延伸链 Extended chain (%)	无规则卷曲 Random curl (%)	SsTPS1	Sspon.01G0009730-1A	514	5.90	59,392.66	72	62	36.28	-0.306	88.29	57.39	11.67	30.93
SsTPS2	Sspon.01G0010010-1A	496	5.29	57,214.41	78	57	37.65	-0.288	94.92	54.03	14.72	31.25
SsTPS3	Sspon.01G0044880-1B	497	5.75	56,761.45	69	54	40.04	-0.312	83.84	49.90	17.51	32.60
SsTPS4	Sspon.04G0011840-1A	735	9.77	81,962.81	74	104	60.66	-0.460	75.17	43.67	8.03	48.30
SsTPS5	Sspon.04G0011840-2B	353	5.78	41,253.51	51	43	47.03	-0.333	87.00	64.31	3.68	32.01
SsTPS6	Sspon.04G0011840-3C	501	6.43	57,711.12	62	58	45.18	-0.311	87.96	55.09	6.99	37.92
SsTPS7	Sspon.05G0015000-1A	581	5.53	66,604.19	78	61	39.07	-0.216	91.02	51.29	12.22	36.49
SsTPS8	Sspon.06G0010490-1P	528	5.33	61,308.56	76	59	39.80	-0.173	98.26	52.65	12.12	35.23
SsTPS9	Sspon.06G0010500-1A	376	5.10	43,465.54	59	41	52.71	-0.328	87.95	52.39	11.17	36.44
SsTPS10	Sspon.06G0011520-1A	433	5.84	49,561.84	62	51	40.69	-0.242	83.44	55.43	9.70	34.87
SsTPS11	Sspon.06G0011680-1A	468	5.32	53,911.93	71	58	49.94	-0.247	97.67	60.47	7.69	31.84
SsTPS12	Sspon.06G0010490-1A	538	5.34	62,470.81	78	61	40.28	-0.201	96.43	50.74	12.64	36.62
SsTPS13	Sspon.06G0021690-1B	325	5.52	37,701.75	51	39	46.74	-0.380	89.14	55.38	12.62	32.00
SsTPS14	Sspon.06G0023410-1B	397	5.47	46,236.32	64	52	38.72	-0.254	96.93	50.13	12.85	37.03
SsTPS15	Sspon.06G0010490-2B	538	5.34	62,468.83	78	61	40.74	-0.192	97.16	50.93	12.64	36.43
SsTPS16	Sspon.06G0011680-2B	607	5.96	70,631.16	81	70	49.39	-0.222	94.23	50.74	13.67	35.58
SsTPS17	Sspon.06G0024130-1B	606	5.97	70,317.44	86	74	53.41	-0.363	83.83	55.61	13.53	30.86
SsTPS18	Sspon.06G0015570-2B	533	5.29	61,439.38	76	57	45.23	-0.220	83.49	50.66	14.82	34.52
SsTPS19	Sspon.06G0023520-2C	491	5.08	57,333.63	76	54	49.64	-0.302	84.83	46.84	13.65	39.51
SsTPS20	Sspon.06G0011660-2C	482	5.04	55,885.74	75	53	48.91	-0.274	86.72	47.51	18.67	33.82
SsTPS21	Sspon.06G0011680-1P	414	5.47	47,542.37	62	48	48.39	-0.301	93.16	53.86	10.63	35.51
SsTPS22	Sspon.06G0030650-1C	358	9.40	41,189.25	38	51	38.63	-0.391	79.55	46.37	13.69	39.94
SsTPS23	Sspon.06G0011680-3C	468	5.15	53,761.70	72	55	51.78	-0.219	98.93	58.55	9.83	31.62
SsTPS24	Sspon.06G0034360-1D	317	4.93	36,898.43	50	32	39.19	-0.134	91.29	48.26	17.98	33.75
SsTPS25	Sspon.06G0011680-2P	333	8.65	38,083.58	38	42	35.22	-0.328	83.75	38.44	21.32	40.24
SsTPS26	Sspon.06G0024130-2D	545	5.56	63,780.15	81	68	48.69	-0.286	90.11	53.39	13.58	33.03
SsTPS27	Sspon.06G0024130-1P	497	5.71	57,610.39	75	65	43.03	-0.181	93.00	51.71	17.30	30.99
SsTPS28	Sspon.06G0011680-4D	328	8.47	37,598.00	37	40	33.44	-0.324	82.65	42.38	17.38	40.24
SsTPS29	Sspon.07G0027750-1B	509	5.55	59,706.37	75	59	55.08	-0.414	90.84	52.65	9.23	38.11
SsTPS30	Sspon.08G0029890-1D	629	5.83	71,361.82	80	67	49.40	-0.160	86.38	46.26	13.67	40.06
SsTPS31	Sspon.01G0039100-1B	348	5.62	39,581.24	49	39	48.50	-0.288	84.74	47.14	12.93	39.66
SsTPS32	Sspon.01G0039100-2C	550	8.25	62,827.75	67	70	54.38	-0.376	82.91	50.36	9.45	40.18
SsTPS33	Sspon.04G0000830-1A	388	5.42	44,015.32	47	38	54.09	-0.088	93.84	55.41	7.47	37.11
SsTPS34	Sspon.05G0030980-1C	545	6.04	62,692.00	73	67	49.04	-0.309	84.02	41.47	12.29	46.24
SsTPS35	Sspon.05G0002480-3D	783	6.03	87,936.45	90	18	46.73	-0.168	89.13	47.51	11.11	41.38
SsTPS36	Sspon.04G0020000-1A	550	5.33	62,580.79	74	53	52.94	-0.116	92.62	49.27	16.55	34.18
SsTPS37	Sspon.04G0019960-2C	515	5.28	59,084.06	71	52	47.41	-0.090	96.37	53.59	14.17	32.23
SsTPS38	Sspon.04G0020000-4P	563	6.13	63,167.56	68	58	50.45	-0.145	89.68	52.75	11.90	35.35
SsTPS39	Sspon.04G0020000-3D	552	5.25	63,021.29	78	56	51.02	-0.123	95.27	54.53	11.78	33.70

Table 2

Fig. 1

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Fig. 8

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基因名称 Gene name	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')
SsTPS5	AGTACCGCATGCCCTACCTA	CACCGCTTCTTGCTGAAACC
SsTPS6	AGTACCGCATGCCCTACCTA	CGCTGCAAGTGTTCCTCAAC
SsTPS8	ATGATGTTCAGGGGCTGCAA	CATGTATGCACGCTTGGGTG
SsTPS10	AGGGGAAAGTGCGTCAGATG	AGTGTGGATACGGCTTAGCG
SsTPS15	AAAAGACCGGACCTTCCACC	CATGTATGCACGCTTGGGTG
SsTPS18	ACCAAGGAGGCATTCGAGTG	CGCGTTCTTCCTCCCTTTCT
SsTPS26	GTCCCATGCACTCCATCACA	GAGGTGGCAACGGTTCCTAA
SsTPS27	AATGGGCTCTCACCTTTCCG	AAGCTTGTTCACGCTCATGC
SsTPS35	TGTTCCTAGGCCATGCAAGG	TTTTGGACTCCGAGTGGCTC
SsTPS36	TGCTGCTGCGCAATGATTTT	GGTCGCTCTTCTGACGTTGA
SsTPS37	GAGCCAAGCCTTGCAGAGTA	ATGGGCAGGCTCTGTAGGTA
SsTPS39	GAAATACCTGTGGTGCGGGA	CCACAACATAGACAAGCGCG
GAPDH	CACGGCCACTGGAAGCA	TCCTCAGGGTTCCTGATGCC

Genome-wide analysis of terpene synthase (TPS) gene family and its expression under biological stress in Saccharum spontaneum

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