绿豆R2R3-MYB转录因子家族鉴定及其类黄酮合成调控基因的筛选

doi:10.3724/SP.J.1006.2025.44077

Abstract

Abstract:

The R2R3-MYB transcription factor family plays a crucial regulatory role in the synthesis of secondary metabolites, stress responses, and the growth and development of plants. This study employed bioinformatics analysis to identify the R2R3-MYB transcription factors across the entire genome of mung bean (Vigna radiata L.), and to predict their physicochemical properties, phylogenetic evolution, chromosome localization, cis-acting elements of the promoter, and gene structure. The expression patterns of mung bean under different tissues, exogenous hormones, and stress conditions were analyzed using transcriptome data and Quantitative Real-time PCR (RT-PCR). R2R3-MYB members potentially involved in regulating the biosynthesis of mung bean flavonoids were screened through correlation analysis and protein interaction networks. The results demonstrated that a total of 168 R2R3-MYB members were identified in mung bean, 145 of which were distributed across 11 chromosomes, while the chromosome information for 23 members remains unknown. Most of these members contain three exons encoding proteins ranging from 99 to 1645 amino acids and are all hydrophilic. The phylogenetic analysis divided the R2R3-MYB gene family in mung beans into 30 subgroups (V1-V30), revealing structural differences among members of different subgroups. Collinearity analysis within the mung bean genome indicated that all segmental duplication events underwent purifying selection. Analysis of cis-acting elements in the promoter regions of the R2R3-MYB genes in mung beans revealed a large number of hormone-responsive and stress-responsive elements, as well as a small number of flavonoid synthesis-responsive elements. Gene expression analysis showed that members with higher expression levels in leaves, petioles, hypocotyls, and seed coats accounted for 15.5%, 16.1%, 16.1%, and 10.7% of the total, respectively. RT-PCR analysis indicated that the relative expression levels of almost all R2R3-MYB family members significantly decreased under low-temperature stress, with different members had diverse response patterns to stress. Protein interaction and correlation analysis suggested that the genes VrMYB6, VrMYB77, and VrMYB93 may be involved in the regulation of flavonoid biosynthesis in mung beans. The results of this study lay the foundation for further in-depth research on the function of the R2R3-MYB transcription factor family in mung beans.

Key words: mung bean, R2R3-MYB transcription factor, transcription factor family analysis, flavonoids synthesis regulatory genes

GUO Fei-Xiang, LI Chun-Xia, ZHOU Shuang, GUO Bin-Bin, ZHANG Jun, MA Chao. Identification of the R2R3-MYB transcription factor family and screening of genes regulating flavonoid synthesis in mung bean[J].Acta Agronomica Sinica, 2025, 51(1): 117-133.

Figures/Tables 15

Table 1

Fig. 1

Table S1

Physicochemical properties and subcellular localization of R2R3-MYB protein in mung bean"

基因名称Gene name	登录号 Accession number	氨基酸数目 No. of amino acids	相对分子质量Molecular weight (Da)	等电点 pI	不稳定指数Instability index	脂肪系数 Aliphatic index	平均疏水指数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
VrMYB2	XP_014490478.1	353	40,519.12	9.42	55.78	62.95	-0.804	细胞核Nuclear
VrMYB154	XP_014490560.1	447	50,974.45	6.72	49.59	66.76	-0.748	细胞核Nuclear
VrMYB155	XP_014490587.1	369	41,290.28	5.72	57.00	67.13	-0.538	细胞核Nuclear
VrMYB29	XP_014491051.2	213	24,945.49	8.91	45.59	69.53	-0.759	细胞核Nuclear
VrMYB156	XP_014491137.1	336	37,977.18	6.28	61.76	62.74	-0.903	细胞核Nuclear
VrMYB157	XP_014491218.1	353	39,488.24	7.66	56.63	64.33	-0.728	细胞核Nuclear
VrMYB158	XP_014491519.1	279	31,560.73	9.00	49.20	71.94	-0.845	细胞核Nuclear
VrMYB159	XP_014491522.1	222	25,046.15	8.77	53.49	67.25	-0.728	细胞核Nuclear
VrMYB17	XP_014491580.1	254	28,186.93	8.97	49.55	76.89	-0.573	细胞核Nuclear
VrMYB160	XP_014491673.1	295	34,124.76	5.06	45.76	68.71	-0.740	细胞核Nuclear
VrMYB161	XP_014491740.1	326	36,571.14	6.17	44.82	82.85	-0.532	细胞核Nuclear
VrMYB162	XP_014492023.1	354	40,197.79	9.31	58.95	72.54	-0.797	细胞核Nuclear
VrMYB163	XP_014492260.1	321	35,431.69	8.65	48.69	75.70	-0.597	细胞核Nuclear
VrMYB164	XP_014492289.1	303	34,033.61	8.06	43.08	76.27	-0.519	细胞核Nuclear
VrMYB165	XP_014492385.1	446	49,816.51	7.19	58.09	59.30	-0.731	细胞核Nuclear
VrMYB166	XP_014492596.1	474	53,985.72	5.67	60.76	75.53	-0.753	细胞核Nuclear
VrMYB167	XP_014492680.1	223	25,598.86	5.71	63.01	73.00	-0.770	细胞核Nuclear
VrMYB24	XP_014493503.1	299	33,897.71	5.28	54.91	64.48	-0.592	细胞核Nuclear
VrMYB20	XP_014493509.1	308	34,486.58	6.32	58.14	61.10	-0.695	细胞核Nuclear
VrMYB30	XP_014493577.1	290	33,354.49	6.06	51.73	66.90	-0.671	细胞核Nuclear
VrMYB25	XP_014493625.1	312	34,732.4	4.95	56.11	60.64	-0.574	细胞核Nuclear
VrMYB27	XP_014493695.1	235	27,330.3	5.71	54.02	54.38	-0.913	细胞核Nuclear
VrMYB21	XP_014493915.2	969	109,225.52	5.34	51.49	70.24	-0.905	细胞核Nuclear
VrMYB18	XP_014494074.1	327	36,606.69	5.52	57.90	65.29	-0.652	细胞核Nuclear
VrMYB7	XP_014494133.1	273	30,238.8	5.75	50.18	72.56	-0.483	细胞核Nuclear
VrMYB8	XP_014494335.1	307	35,125.92	5.25	66.86	63.91	-0.764	细胞核Nuclear
VrMYB36	XP_014494877.1	404	45,089.38	6.64	58.14	67.65	-0.688	细胞核Nuclear
VrMYB35	XP_014495060.1	365	41,348.45	6.28	66.58	73.95	-0.571	细胞核Nuclear
VrMYB39	XP_014495171.1	393	43,217.33	8.15	51.87	69.72	-0.610	细胞核Nuclear
VrMYB37	XP_014495198.1	439	48,752.97	6.96	50.96	59.54	-0.752	细胞核Nuclear
VrMYB38	XP_014495343.1	296	34,406.21	8.88	58.65	67.84	-0.690	细胞核Nuclear
VrMYB40	XP_014495344.2	356	40,586.71	8.50	59.69	61.66	-0.827	细胞核Nuclear
VrMYB32	XP_014495439.1	334	37,009.28	6.89	47.45	61.35	-0.583	细胞核Nuclear
VrMYB33	XP_014495618.1	353	40,571.45	5.99	51.36	63.51	-0.874	细胞核Nuclear
VrMYB34	XP_014495632.1	276	30,743.97	5.78	50.45	63.66	-0.615	细胞核Nuclear
VrMYB31	XP_014495663.1	288	32,668.16	5.01	59.29	67.08	-0.835	细胞核Nuclear
VrMYB49	XP_014496414.1	393	43,324.87	6.37	52.14	60.84	-0.782	细胞核Nuclear
VrMYB51	XP_014496890.2	266	30,532.81	7.57	46.40	62.63	-0.666	细胞核Nuclear
VrMYB48	XP_014497102.1	317	35,260.48	8.50	57.97	60.38	-0.735	细胞核Nuclear
VrMYB50	XP_014497212.1	233	26,831.1	7.65	42.12	68.97	-0.804	细胞核Nuclear
VrMYB43	XP_014497533.1	332	37,589.87	6.98	61.47	59.61	-0.757	细胞核Nuclear
VrMYB47	XP_014497557.1	308	34,506.71	8.13	50.69	68.15	-0.675	细胞核Nuclear
VrMYB45	XP_014497590.1	202	23,404.17	6.52	54.95	61.83	-0.972	细胞核Nuclear
VrMYB44	XP_014497598.1	199	23,201.05	7.71	54.97	60.80	-0.897	细胞核Nuclear
VrMYB42	XP_014497919.1	245	28,010.17	5.85	51.28	51.31	-0.923	细胞核Nuclear
VrMYB63	XP_014498875.1	510	56,364.71	4.83	46.30	62.55	-0.688	细胞核Nuclear
VrMYB55	XP_014499283.1	332	37,079.62	6.07	54.79	72.62	-0.584	细胞核Nuclear
VrMYB64	XP_014499553.1	465	51,891.28	5.94	41.56	53.76	-0.956	细胞核Nuclear
VrMYB65	XP_014499570.1	241	27,677.98	6.23	57.75	71.62	-0.715	细胞核Nuclear
VrMYB59	XP_014499816.1	258	28,897.52	8.02	56.23	74.22	-0.753	细胞核Nuclear
VrMYB54	XP_014500310.1	341	38,408	5.84	54.17	72.55	-0.502	细胞核Nuclear
VrMYB61	XP_014500370.1	356	40,431.79	5.04	58.23	58.37	-0.887	细胞核Nuclear
VrMYB57	XP_014500397.1	255	28,177.83	9.58	49.35	64.67	-0.788	细胞核Nuclear
VrMYB52	XP_014500543.1	168	19,498.29	8.86	54.24	73.63	-0.783	细胞核Nuclear
VrMYB58	XP_014500580.2	316	35,386.47	8.61	51.44	73.16	-0.592	细胞核Nuclear
VrMYB56	XP_014500680.1	341	37,894.6	7.49	56.44	74.05	-0.581	细胞核Nuclear
VrMYB62	XP_014500689.1	354	39,760.86	6.11	52.36	85.65	-0.392	细胞核Nuclear
VrMYB60	XP_014500881.2	335	38,140.87	6.56	58.61	75.94	-0.566	细胞核Nuclear
VrMYB67	XP_014502988.1	229	26,557.09	9.76	60.71	70.70	-0.881	细胞核Nuclear
VrMYB71	XP_014503089.1	322	37,763.5	9.03	57.96	61.74	-0.966	细胞核Nuclear
VrMYB74	XP_014503316.1	239	26,950.93	7.90	50.64	58.49	-0.765	细胞核Nuclear
VrMYB77	XP_014503606.1	274	30,061.36	9.53	64.13	73.39	-0.546	细胞核Nuclear
VrMYB70	XP_014503716.1	322	36,800.06	4.90	52.65	69.97	-0.794	细胞核Nuclear
VrMYB76	XP_014504095.1	368	41,266.76	6.41	55.04	62.26	-0.761	细胞核Nuclear
VrMYB75	XP_014504137.1	311	34,682.76	8.10	57.24	63.38	-0.706	细胞核Nuclear
VrMYB69	XP_014504153.2	300	34,017.88	9.40	59.97	85.57	-0.54	细胞核Nuclear
VrMYB73	XP_014504193.2	415	46,264.58	6.50	51.14	73.28	-0.622	细胞核Nuclear
VrMYB78	XP_014504223.1	195	21,915.55	10.44	44.37	63.54	-0.756	细胞核Nuclear
VrMYB68	XP_014505357.1	317	36,325.3	8.12	51.91	66.72	-0.572	细胞核Nuclear
VrMYB72	XP_014505359.1	304	34,265.21	8.73	56.32	57.7	-0.796	细胞核Nuclear
VrMYB84	XP_014505860.1	288	31,053.32	9.06	60.05	72.19	-0.322	细胞核Nuclear
VrMYB5	XP_014506098.1	319	35,110.35	8.42	51.17	68.56	-0.602	细胞核Nuclear
VrMYB82	XP_014506348.1	399	44,463.32	5.96	54.87	71.15	-0.614	细胞核Nuclear
VrMYB10	XP_014506438.1	356	40,139.75	6.08	51.58	60.81	-0.735	细胞核Nuclear
VrMYB93	XP_014506712.1	245	27,984.64	8.13	51.43	75.63	-0.718	细胞核Nuclear
VrMYB95	XP_014506748.1	164	19,056.48	9.62	51.35	82.07	-0.598	细胞核Nuclear
VrMYB97	XP_014506838.2	298	33,320.62	7.06	44.96	78.79	-0.399	细胞核Nuclear
VrMYB81	XP_014506887.1	450	49,710.71	6.06	57.01	54.89	-0.729	细胞核Nuclear
VrMYB91	XP_014507110.1	295	32,234.85	6.98	65.33	59.86	-0.625	细胞核Nuclear
VrMYB89	XP_014507552.1	363	41,251.18	5.94	53.28	69.56	-0.658	细胞核Nuclear
VrMYB13	XP_014507919.1	555	60,998.53	5.57	45.88	64.88	-0.653	细胞核Nuclear
VrMYB94	XP_014507961.1	306	35,249.24	6.10	53.99	71.70	-0.769	细胞核Nuclear
VrMYB79	XP_014508007.1	225	25,636.07	9.08	55.83	80.22	-0.764	细胞核Nuclear
VrMYB90	XP_014508126.1	312	35,048.85	6.16	50.92	58.85	-0.774	细胞核Nuclear
VrMYB9	XP_014508294.1	229	26,995.16	8.51	60.83	60.00	-0.913	细胞核Nuclear
VrMYB92	XP_014508343.1	361	40,704.39	6.16	65.30	69.47	-0.751	细胞核Nuclear
VrMYB86	XP_014508468.1	198	22,943.64	6.60	57.67	74.39	-0.908	细胞核Nuclear
VrMYB80	XP_014508588.1	488	55,604.67	5.91	58.75	63.95	-0.988	细胞核Nuclear
VrMYB11	XP_014509201.2	358	39,544.61	8.89	56.66	67.54	-0.563	细胞核Nuclear
VrMYB87	XP_014509393.1	271	30,693.45	5.15	50.54	78.12	-0.68	细胞核Nuclear
VrMYB4	XP_014509585.2	399	44,662.72	7.64	51.72	79.65	-0.357	细胞核Nuclear
VrMYB15	XP_014509801.1	289	32,833.83	7.22	50.58	67.82	-0.757	细胞核Nuclear
VrMYB3	XP_014509861.2	251	29,115.64	7.87	63.01	73.47	-0.795	细胞核Nuclear
VrMYB96	XP_014510156.1	314	36,063.06	6.21	58.06	62.74	-0.836	细胞核Nuclear
VrMYB107	XP_014510965.1	262	30,135.86	5.67	65.63	60.34	-0.730	细胞核Nuclear
VrMYB100	XP_014511116.1	258	29,566.02	5.62	62.92	59.34	-0.777	细胞核Nuclear
VrMYB110	XP_014511901.1	273	31,804.92	6.21	44.41	63.22	-0.815	细胞核Nuclear
VrMYB112	XP_014511935.1	318	35,764.29	6.96	53.02	74.47	-0.584	细胞核Nuclear
VrMYB106	XP_014512306.1	395	44,483.57	6.60	54.21	65.42	-0.727	细胞核Nuclear
VrMYB104	XP_014512332.1	257	29,779.93	9.01	55.67	81.56	-0.589	细胞核Nuclear
VrMYB115	XP_014512566.1	241	27,351.48	6.14	51.98	61.83	-0.764	细胞核Nuclear
VrMYB102	XP_014512645.1	316	34,687.82	7.09	53.99	60.44	-0.615	细胞核Nuclear
VrMYB14	XP_014512651.1	969	109,188.43	5.36	51.82	70.72	-0.901	细胞核Nuclear
VrMYB114	XP_014512719.1	305	34,691	6.40	66.70	63.67	-0.730	细胞核Nuclear
VrMYB103	XP_014512761.1	360	40,628.34	5.20	55.12	76.36	-0.696	细胞核Nuclear
VrMYB101	XP_014513141.2	287	33,282.66	9.34	63.54	65.3	-0.805	细胞核Nuclear
VrMYB109	XP_014513204.1	261	30,035.61	5.10	53.88	70.27	-0.734	细胞核Nuclear
VrMYB105	XP_014513208.1	342	39,172.99	7.07	49.85	69.85	-0.863	细胞核Nuclear
VrMYB113	XP_014513226.1	269	30,264.31	9.01	64.58	70.04	-0.662	细胞核Nuclear
VrMYB108	XP_014513230.1	267	31,116.31	9.11	61.24	69.4	-0.842	细胞核Nuclear
VrMYB111	XP_014513300.1	1645	179,255.55	5.82	45.51	68.21	-0.614	细胞核Nuclear
VrMYB124	XP_014515157.1	352	38,079.81	6.23	46.47	73.75	-0.499	细胞核Nuclear
VrMYB116	XP_014515250.1	326	37,097.32	6.19	58.75	78.10	-0.453	细胞核Nuclear
VrMYB117	XP_014515266.1	266	30,146.01	8.45	60.91	74.77	-0.852	细胞核Nuclear
VrMYB118	XP_014515267.1	292	33,211.04	6.46	54.81	65.75	-0.819	细胞核Nuclear
VrMYB121	XP_014515433.2	320	36,512.18	5.86	65.81	68.84	-0.679	细胞核Nuclear
VrMYB125	XP_014515544.1	355	40,712.28	9.36	60.40	60.14	-0.721	细胞核Nuclear
VrMYB123	XP_014515811.1	350	40,036.64	8.96	64.20	75.63	-0.787	细胞核Nuclear
VrMYB120	XP_014516586.1	279	31,167.14	8.08	56.03	72.08	-0.623	细胞核Nuclear
VrMYB122	XP_014516816.1	359	41,072.71	9.12	56.34	75.68	-0.784	细胞核Nuclear
VrMYB126	XP_014517284.1	325	36,384.97	6.63	41.73	65.48	-0.631	细胞核Nuclear
VrMYB127	XP_014517297.1	412	46,439.76	9.05	51.83	70.29	-0.771	细胞核Nuclear
VrMYB129	XP_014517902.1	374	42,214.16	5.94	53.07	71.66	-0.627	细胞核Nuclear
VrMYB6	XP_014518062.1	408	44,437.02	5.93	56.82	63.41	-0.738	细胞核Nuclear
VrMYB130	XP_014518874.1	637	72,923.96	8.81	53.79	64.69	-1.002	细胞核Nuclear
VrMYB128	XP_014518898.1	320	36,181.58	9.07	59.95	60.34	-0.763	过氧化物酶体Peroxisomal
VrMYB19	XP_014519116.1	259	30,015.36	5.47	66.20	59.85	-0.822	细胞核Nuclear
VrMYB135	XP_014519579.1	179	20,859.68	9.53	56.90	71.40	-0.761	细胞核Nuclear
VrMYB139	XP_014519753.2	454	50,306.7	7.58	53.06	53.72	-0.701	细胞核Nuclear
VrMYB141	XP_014519828.1	284	31,907.4	8.87	46.97	84.15	-0.471	细胞核Nuclear
VrMYB142	XP_014519830.1	301	33,798.42	8.72	51.25	80.7	-0.509	细胞核Nuclear
VrMYB143	XP_014519831.1	284	31,877.33	8.87	46.12	82.78	-0.490	细胞核Nuclear
VrMYB144	XP_014519832.2	316	36,076.88	8.91	46.6	75.28	-0.684	细胞核Nuclear
VrMYB145	XP_014519833.1	301	33,771.47	7.68	44.26	79.73	-0.516	细胞核Nuclear
VrMYB134	XP_014520093.1	265	30,135.08	5.45	47.93	79.51	-0.649	细胞核Nuclear
VrMYB136	XP_014520380.1	350	39,831.52	6.75	49.12	65.94	-0.725	细胞核Nuclear
VrMYB138	XP_014520455.2	344	38,854.71	6.80	47.11	73.72	-0.697	细胞核Nuclear
VrMYB132	XP_014520504.1	303	34,206.42	6.59	44.17	77.59	-0.628	细胞核Nuclear
VrMYB140	XP_014520829.1	293	31,226.46	6.37	49.98	65.26	-0.600	细胞核Nuclear
VrMYB137	XP_014521508.1	310	33,733.35	9.27	54.04	73.55	-0.454	细胞核Nuclear
VrMYB146	XP_014521761.1	274	30,927.32	7.72	57.48	65.88	-0.836	细胞核Nuclear
VrMYB147	XP_014522324.1	336	38,530.62	8.97	60.39	55.74	-0.913	细胞核Nuclear
VrMYB16	XP_014523159.1	341	38,315.16	5.69	51.97	78.09	-0.543	细胞核Nuclear
VrMYB148	XP_014523541.1	286	32,163.29	5.92	53.84	82.83	-0.486	细胞核Nuclear
VrMYB149	XP_014523608.1	294	31,231.63	6.80	48.98	63.44	-0.645	细胞核Nuclear
VrMYB26	XP_014523889.1	224	25,899.25	6.72	43.28	70.62	-0.696	细胞核Nuclear
VrMYB28	XP_014523933.1	308	34,382.87	6.00	51.64	81.40	-0.483	细胞核Nuclear
VrMYB151	XP_014524064.1	374	42,360.42	8.81	53.39	74.30	-0.697	细胞核Nuclear
VrMYB152	XP_014524093.1	289	32,729.65	7.63	51.61	60.10	-0.979	细胞核Nuclear
VrMYB153	XP_014524479.1	526	58,840.02	5.33	49.40	65.48	-0.785	细胞核Nuclear
VrMYB133	XP_022631357.1	510	57,347.4	8.18	55.01	62.53	-0.676	细胞核Nuclear
VrMYB131	XP_022631644.1	397	45,022.38	6.94	42.96	88.14	-0.276	细胞核Nuclear
VrMYB150	XP_022632584.1	514	56,968.81	5.79	56.18	58.11	-0.746	细胞核Nuclear
VrMYB168	XP_022633932.1	386	43,709.37	6.28	57.89	67.98	-0.710	细胞核Nuclear
VrMYB12	XP_022634264.1	378	41,994.41	8.73	54.57	74.05	-0.513	细胞核Nuclear
VrMYB22	XP_022634320.1	99	11,526.34	10.4	66.57	66.06	-0.810	细胞核Nuclear
VrMYB23	XP_022634322.1	219	25,489.04	5.56	48.64	59.77	-0.859	细胞核Nuclear
VrMYB41	XP_022635231.1	312	35,451.36	5.52	58.31	59.04	-0.720	细胞核Nuclear
VrMYB46	XP_022635873.1	225	26,354.19	9.61	68.18	64.98	-0.902	细胞核Nuclear
VrMYB53	XP_022635910.1	415	47,715.06	6.29	54.57	69.54	-0.708	细胞核Nuclear
VrMYB66	XP_022637422.1	237	27,126.58	9.59	54.49	63.33	-0.965	细胞核Nuclear
VrMYB88	XP_022638520.1	189	21,854.73	8.97	49.45	63.39	-0.801	细胞核Nuclear
VrMYB98	XP_022638546.1	525	58,703.1	7.22	53.37	66.48	-0.613	细胞核Nuclear
VrMYB99	XP_022638786.1	282	31,347.02	8.47	52.64	66.77	-0.819	细胞核Nuclear
VrMYB83	XP_022640184.1	241	27,649.18	8.11	52.17	66.76	-0.822	细胞核Nuclear
VrMYB85	XP_022640223.1	304	34,030.11	7.62	55.37	69.31	-0.584	细胞核Nuclear
VrMYB1	XP_022640949.1	266	30,826.14	9.73	53.47	60.15	-0.840	细胞核Nuclear
VrMYB119	XP_022641765.1	294	32,984.46	8.52	55.40	77.69	-0.540	细胞核Nuclear

Table S1

Table S2

Secondary structure prediction of R2R3-MYB protein in mung bean"

登录号 Accession number	α-螺旋 Alpha helix (Hh)	β-转角 Beta turn (Tt)	延伸链 Extended strand (Ee)	无规则卷曲 Random coil (Cc)
XP_014490478.1	28.05	4.82	10.76	56.37
XP_014490560.1	28.41	2.91	6.04	62.64
XP_014490587.1	32.25	3.79	7.05	56.91
XP_014491051.2	43.19	6.10	1.88	48.83
XP_014491137.1	34.52	2.08	5.65	57.74
XP_014491218.1	27.76	5.67	10.20	56.37
XP_014491519.1	36.20	4.66	14.70	44.44
XP_014491522.1	29.73	6.76	6.76	56.76
XP_014491580.1	33.46	5.12	10.24	51.18
XP_014491673.1	37.29	5.76	5.08	51.86
XP_014491740.1	30.98	2.45	7.67	58.90
XP_014492023.1	53.67	2.26	3.95	40.11
XP_014492260.1	18.69	4.67	14.33	62.31
XP_014492289.1	38.28	5.28	8.25	48.18
XP_014492385.1	28.70	3.36	3.59	64.35
XP_014492596.1	36.92	4.01	7.59	51.48
XP_014492680.1	41.70	5.38	4.93	47.98
XP_014493503.1	30.43	4.35	8.70	56.52
XP_014493509.1	23.05	5.19	13.31	58.44
XP_014493577.1	31.72	5.52	6.55	56.21
XP_014493625.1	35.90	5.45	5.45	53.21
XP_014493695.1	40.85	6.81	6.81	45.53
XP_014493915.2	53.66	1.75	4.44	40.14
XP_014494074.1	28.75	5.20	8.87	57.19
XP_014494133.1	31.87	7.69	14.65	45.79
XP_014494335.1	32.25	4.56	8.47	54.72
XP_014494877.1	32.18	3.96	4.70	59.16
XP_014495060.1	34.25	8.22	14.52	43.01
XP_014495171.1	35.11	7.12	11.70	46.06
XP_014495198.1	25.97	4.10	4.56	65.38
XP_014495343.1	28.72	4.73	17.57	48.99
XP_014495344.2	29.21	2.53	8.15	60.11
XP_014495439.1	27.54	7.78	16.77	47.90
XP_014495618.1	35.13	5.95	15.01	43.91
XP_014495632.1	40.58	5.07	9.42	44.93
XP_014495663.1	35.07	3.47	5.21	56.25
XP_014496414.1	33.59	5.09	10.43	50.89
XP_014496890.2	37.59	6.02	7.89	48.50
XP_014497102.1	36.59	0.63	4.42	58.36
XP_014497212.1	35.62	9.44	9.44	45.49
XP_014497533.1	23.19	4.82	13.86	58.13
XP_014497557.1	36.04	5.84	6.82	51.30
XP_014497590.1	33.66	5.45	5.45	55.45
XP_014497598.1	33.17	5.03	6.03	55.78
XP_014497919.1	38.37	6.12	6.94	48.57
XP_014498875.1	26.27	4.12	6.47	63.14
XP_014499283.1	35.84	3.31	1.20	59.64
XP_014499553.1	26.45	4.09	4.73	64.73
XP_014499570.1	32.78	5.39	7.05	54.77
XP_014499816.1	34.11	6.20	6.98	52.71
XP_014500310.1	33.43	3.81	5.57	57.18
XP_014500370.1	27.25	4.78	8.15	59.83
XP_014500397.1	38.43	5.10	7.84	48.63
XP_014500543.1	38.10	9.52	1.79	50.60
XP_014500580.2	31.33	6.96	19.94	41.77
XP_014500680.1	31.09	2.93	6.16	59.82
XP_014500689.1	29.94	4.52	6.50	59.04
XP_014500881.2	42.09	5.07	8.36	44.48
XP_014502988.1	44.10	6.99	7.86	41.05
XP_014503089.1	27.02	4.35	15.22	53.42
XP_014503316.1	31.80	5.44	7.95	54.81
XP_014503606.1	33.58	3.65	5.84	56.93
XP_014503716.1	33.54	4.35	6.21	55.90
XP_014504095.1	26.09	4.62	13.59	55.71
XP_014504137.1	27.65	5.79	7.07	59.49
XP_014504153.2	28.33	5.00	10.33	56.33
XP_014504193.2	22.65	6.02	14.22	57.11
XP_014504223.1	40.51	7.69	7.18	44.62
XP_014505357.1	30.91	4.10	13.88	51.10
XP_014505359.1	20.39	3.29	17.76	58.55
XP_014505860.1	29.51	3.82	5.56	61.11
XP_014506098.1	33.54	2.51	4.08	59.87
XP_014506348.1	34.59	3.51	8.02	53.88
XP_014506438.1	26.97	4.21	8.99	59.83
XP_014506712.1	34.29	6.12	6.94	52.65
XP_014506748.1	50.00	6.71	6.71	36.59
XP_014506838.2	24.50	11.07	18.12	46.31
XP_014506887.1	24.44	3.78	10.22	61.56
XP_014507110.1	20.00	2.03	12.20	65.76
XP_014507552.1	33.61	3.31	6.89	56.20
XP_014507919.1	25.41	3.60	4.32	66.67
XP_014507961.1	37.58	2.61	6.21	53.59
XP_014508007.1	25.33	6.22	5.78	62.67
XP_014508126.1	34.62	2.88	4.49	58.01
XP_014508294.1	31.88	4.37	8.73	55.02
XP_014508343.1	36.01	2.22	4.71	57.06
XP_014508468.1	44.95	6.57	3.54	44.95
XP_014508588.1	36.68	3.28	8.61	51.43
XP_014509201.2	23.18	5.03	11.73	60.06
XP_014509393.1	36.53	4.06	5.90	53.51
XP_014509585.2	33.83	4.76	6.52	54.89
XP_014509801.1	32.53	5.54	4.50	57.44
XP_014509861.2	36.25	5.18	4.78	53.78
XP_014510156.1	32.17	3.82	4.78	59.24
XP_014510965.1	22.14	4.96	4.58	68.32
XP_014511116.1	30.62	4.26	3.10	62.02
XP_014511901.1	43.22	4.03	5.13	47.62
XP_014511935.1	34.91	6.29	12.89	45.91
XP_014512306.1	26.84	4.81	3.04	65.32
XP_014512332.1	39.30	6.61	9.34	44.75
XP_014512566.1	23.24	3.32	14.11	59.34
XP_014512645.1	25.00	6.33	12.66	56.01
XP_014512651.1	53.66	2.68	4.54	39.11
XP_014512719.1	31.48	3.93	6.56	58.03
XP_014512761.1	35.83	5.00	13.06	46.11
XP_014513141.2	47.04	8.36	9.06	35.54
XP_014513204.1	38.31	4.98	5.75	50.96
XP_014513208.1	39.77	5.26	3.80	51.17
XP_014513226.1	37.92	5.58	5.58	50.93
XP_014513230.1	48.69	5.62	8.99	36.70
XP_014513300.1	26.32	2.37	9.42	61.88
XP_014515157.1	25.28	3.98	11.08	59.66
XP_014515250.1	26.99	4.60	3.99	64.42
XP_014515266.1	27.44	6.39	3.38	62.78
XP_014515267.1	29.11	6.85	7.88	56.16
XP_014515433.2	23.75	3.75	9.38	63.12
XP_014515544.1	29.58	5.35	15.49	49.58
XP_014515811.1	56.00	4.00	4.00	36.00
XP_014516586.1	34.41	4.30	5.73	55.56
XP_014516816.1	56.55	4.18	5.29	33.98
XP_014517284.1	35.06	5.54	5.54	53.85
XP_014517297.1	30.34	4.61	9.95	55.10
XP_014517902.1	27.54	4.28	5.61	62.57
XP_014518062.1	29.41	3.92	9.31	57.35
XP_014518874.1	59.34	3.14	5.81	31.71
XP_014518898.1	22.81	2.81	14.06	60.31
XP_014519116.1	28.19	8.49	11.58	51.47
XP_014519579.1	41.90	7.82	7.26	43.02
XP_014519753.2	25.55	3.74	11.89	58.81
XP_014519828.1	38.38	4.93	7.75	48.94
XP_014519830.1	38.54	4.98	13.29	43.19
XP_014519831.1	41.20	5.99	8.10	44.72
XP_014519832.2	36.71	5.70	9.18	48.42
XP_014519833.1	36.88	3.99	11.63	47.51
XP_014520093.1	34.72	4.53	5.66	55.09
XP_014520380.1	36.86	4.57	9.14	49.43
XP_014520455.2	39.53	4.94	4.65	50.87
XP_014520504.1	43.56	3.30	5.94	47.19
XP_014520829.1	18.09	4.44	12.63	64.85
XP_014521508.1	25.48	3.87	6.77	63.87
XP_014521761.1	24.82	4.01	4.01	67.15
XP_014522324.1	25.60	2.38	10.42	61.61
XP_014523159.1	41.06	3.81	3.52	51.61
XP_014523541.1	41.26	5.59	9.79	43.36
XP_014523608.1	19.05	3.74	12.24	64.97
XP_014523889.1	31.70	7.14	6.70	54.46
XP_014523933.1	35.71	3.25	5.52	55.52
XP_014524064.1	35.83	5.08	8.29	50.80
XP_014524093.1	26.64	4.84	12.11	56.40
XP_014524479.1	22.43	3.04	5.89	68.63
XP_022631357.1	27.45	4.90	6.27	61.37
XP_022631644.1	41.31	4.53	13.60	40.55
XP_022632584.1	19.07	5.06	10.51	65.37
XP_022633932.1	32.90	4.40	6.99	55.70
XP_022634264.1	25.93	6.61	12.43	55.03
XP_022634320.1	33.33	9.09	8.08	49.49
XP_022634322.1	36.07	5.94	4.11	53.88
XP_022635231.1	36.22	4.49	8.01	51.28
XP_022635873.1	54.22	4.89	3.11	37.78
XP_022635910.1	30.84	3.61	8.67	56.87
XP_022637422.1	31.22	5.49	9.28	54.01
XP_022638520.1	41.80	11.11	10.05	37.04
XP_022638546.1	25.52	5.33	8.76	60.38
XP_022638786.1	25.18	5.32	16.67	52.84
XP_022640184.1	42.74	7.05	6.22	43.98
XP_022640223.1	35.20	3.29	8.55	52.96
XP_022640949.1	40.98	4.89	12.03	42.11
XP_022641765.1	39.80	4.42	8.16	47.62

Table S2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Table S3

Ka/Ks analysis of R2R3-MYB gene in mung bean"

序列1 Seq_1	序列2 Seq_2	非同义替换率 Ka	同义替换率 Ks	非同义替换/同义替换Ka/Ks	有效长度Effective length	平均S位点Average S-sites	平均N位点Average N-sites	cN	cS	pN	pS
rna-XM_022780189.1	rna-XM_014635074.1	0.3078	1.0338	0.2977	1224	256.0833	967.9167	244.3333	143.6667	0.2524	0.5610
rna-XM_022780566.1	rna-XM_014635101.2	0.1261	0.8136	0.1550	999	215.6667	783.3333	90.9167	107.0833	0.1161	0.4965
rna-XM_014668607.2	rna-XM_014636033.2	0.1720	1.1622	0.1480	822	180.0000	642.0000	98.6667	106.3333	0.1537	0.5907
rna-XM_014636094.2	rna-XM_014644330.2	0.1882	0.7434	0.2531	750	167.6667	582.3333	96.9167	79.0833	0.1664	0.4717
rna-XM_022778602.1	rna-XM_014636187.2	0.4748	1.7940	0.2647	480	103.5833	376.4167	132.4167	70.5833	0.3518	0.6814
rna-XM_014668447.2	rna-XM_014636254.2	0.2000	1.1215	0.1783	891	195.9167	695.0833	122.0000	114.0000	0.1755	0.5819
rna-XM_014662416.2	rna-XM_014636254.2	0.3013	2.6518	0.1136	936	207.8333	728.1667	180.6667	151.3333	0.2481	0.7281
rna-XM_014639391.2	rna-XM_014636899.2	0.1446	0.7099	0.2037	1191	257.6667	933.3333	122.7500	118.2500	0.1315	0.4589
rna-XM_014638588.2	rna-XM_014638091.2	0.3803	2.2624	0.1681	855	175.8333	679.1667	202.5833	125.4167	0.2983	0.7133
rna-XM_014638588.2	rna-XM_022779510.1	0.1619	0.6790	0.2384	921	192.3333	728.6667	106.0833	85.9167	0.1456	0.4467
rna-XM_014638647.2	rna-XM_014651352.2	0.2537	0.9661	0.2626	729	168.0000	561.0000	120.7500	91.2500	0.2152	0.5432
rna-XM_014638849.2	rna-XM_014654670.2	0.2541	0.5574	0.4558	897	189.4167	707.5833	152.5000	74.5000	0.2155	0.3933
rna-XM_014639712.2	rna-XM_014656820.2	0.1598	0.8404	0.1902	1161	254.2500	906.7500	130.5000	128.5000	0.1439	0.5054
rna-XM_014639857.2	rna-XM_014649871.2	0.1444	0.8683	0.1663	867	185.6667	681.3333	89.5000	95.5000	0.1314	0.5144
rna-XM_014639858.2	rna-XM_014641616.2	0.2792	2.6718	0.1045	870	188.0000	682.0000	159.0000	137.0000	0.2331	0.7287
rna-XM_014639858.2	rna-XM_014652640.2	0.1876	0.9742	0.1926	879	190.0833	688.9167	114.3333	103.6667	0.1660	0.5454
rna-XM_022778513.1	rna-XM_014639953.2	0.3419	1.3375	0.2556	888	186.5833	701.4167	192.5833	116.4167	0.2746	0.6239
rna-XM_014639953.2	rna-XM_014642047.2	0.3041	1.4189	0.2144	930	195.0833	734.9167	183.7500	124.2500	0.2500	0.6369
rna-XM_014640132.2	rna-XM_014657275.2	0.3539	1.4057	0.2518	975	201.0833	773.9167	218.3333	127.6667	0.2821	0.6349
rna-XM_014640928.2	rna-XM_014650862.2	0.4346	2.4693	0.1760	1110	244.4167	865.5833	285.5000	176.5000	0.3298	0.7221
rna-XM_014640928.2	rna-XM_022778211.1	0.3640	2.6801	0.1358	1050	229.6667	820.3333	236.5833	167.4167	0.2884	0.7290
rna-XM_014650612.2	rna-XM_014641616.2	0.1659	0.6854	0.2421	909	202.0000	707.0000	105.2500	90.7500	0.1489	0.4493
rna-XM_014641616.2	rna-XM_014652640.2	0.3519	2.1240	0.1657	861	188.6667	672.3333	188.8333	133.1667	0.2809	0.7058
rna-XM_022778513.1	rna-XM_014642047.2	0.1696	0.6238	0.2719	900	192.8333	707.1667	107.3333	81.6667	0.1518	0.4235
rna-XM_014642071.2	rna-XM_022786044.1	0.1376	0.6361	0.2163	822	180.3333	641.6667	80.6667	77.3333	0.1257	0.4288
rna-XM_014654375.2	rna-XM_014642112.2	0.1436	1.0902	0.1317	597	123.8333	473.1667	61.8333	71.1667	0.1307	0.5747
rna-XM_014663630.2	rna-XM_014642433.1	0.1817	0.7792	0.2331	693	149.0833	543.9167	87.7500	72.2500	0.1613	0.4846
rna-XM_022778602.1	rna-XM_014644084.2	0.5350	3.0374	0.1761	438	97.2500	340.7500	130.3333	71.6667	0.3825	0.7369
rna-XM_014644084.2	rna-XM_014666481.1	0.6052	2.3371	0.2590	294	62.6667	231.3333	96.0833	44.9167	0.4153	0.7168
rna-XM_014644911.2	rna-XM_014648120.2	0.2610	1.8531	0.1408	705	164.3333	540.6667	119.1667	112.8333	0.2204	0.6866
rna-XM_014644911.2	rna-XM_014650374.2	0.3289	1.5390	0.2137	717	170.5833	546.4167	145.5000	111.5000	0.2663	0.6536
rna-XM_014644911.2	rna-XM_014666022.2	0.3525	3.9120	0.0901	753	179.0833	573.9167	161.4167	133.5833	0.2813	0.7459
rna-XM_014645057.2	rna-XM_014651226.2	0.3277	1.8294	0.1791	432	96.1667	335.8333	89.1667	65.8333	0.2655	0.6846
rna-XM_014645094.2	rna-XM_014648737.2	0.2350	1.3692	0.1716	537	132.5833	404.4167	81.5833	83.4167	0.2017	0.6292
rna-XM_014645194.2	rna-XM_014648609.2	0.1841	1.1619	0.1584	966	211.3333	754.6667	123.1667	124.8333	0.1632	0.5907
rna-XM_014645194.2	rna-XM_022784502.1	0.3845	2.3274	0.1652	867	193.0000	674.0000	202.7500	138.2500	0.3008	0.7163
rna-XM_014647830.1	rna-XM_014666838.2	0.3694	1.1299	0.3269	690	149.7500	540.2500	157.5833	87.4167	0.2917	0.5838
rna-XM_014648120.2	rna-XM_014650374.2	0.2990	1.7962	0.1664	798	191.3333	606.6667	149.5833	130.4167	0.2466	0.6816
rna-XM_014649873.2	rna-XM_014663412.2	0.1160	0.8302	0.1397	903	202.6667	700.3333	75.2500	101.7500	0.1074	0.5021
rna-XM_014650374.2	rna-XM_014666022.2	0.1378	1.4860	0.0928	828	199.2500	628.7500	79.1667	128.8333	0.1259	0.6466
rna-XM_014650862.2	rna-XM_022778211.1	0.2296	1.3801	0.1663	1059	227.5833	831.4167	164.4167	143.5833	0.1978	0.6309
rna-XM_014651226.2	rna-XM_022786210.1	0.3563	3.1262	0.1140	582	127.4167	454.5833	128.9167	94.0833	0.2836	0.7384
rna-XM_014651401.2	rna-XM_014664267.2	0.2406	0.7111	0.3383	1257	281.1667	975.8333	200.8333	129.1667	0.2058	0.4594
rna-XM_014668447.2	rna-XM_014652066.2	0.3394	4.3051	0.0788	900	198.7500	701.2500	191.4167	148.5833	0.2730	0.7476
rna-XM_014652066.2	rna-XM_014662416.2	0.2332	0.9009	0.2588	1068	237.5833	830.4167	166.4167	124.5833	0.2004	0.5244
rna-XM_022778990.1	rna-XM_014652475.2	0.4088	2.6224	0.1559	786	177.8333	608.1667	191.6667	129.3333	0.3152	0.7273
rna-XM_014652475.2	rna-XM_014662545.2	0.1670	1.3036	0.1281	888	192.2500	695.7500	104.1667	118.8333	0.1497	0.6181
rna-XM_014652982.2	rna-XM_014664093.2	0.2184	1.2977	0.1683	504	106.4167	397.5833	75.3333	65.6667	0.1895	0.6171
rna-XM_014653907.2	rna-XM_014664607.2	0.0907	0.6921	0.1310	792	167.4167	624.5833	53.3333	75.6667	0.0854	0.4520
rna-XM_014654670.2	rna-XM_014657233.2	0.4056	1.9651	0.2064	846	177.8333	668.1667	209.3333	123.6667	0.3133	0.6954
rna-XM_014655630.2	rna-XM_014655479.2	0.1305	0.8446	0.1545	732	153.2500	578.7500	69.3333	77.6667	0.1198	0.5068
rna-XM_014655630.2	rna-XM_014636037.2	0.3851	1.4787	0.2604	633	131.6667	501.3333	151.0000	85.0000	0.3012	0.6456
rna-XM_022784502.1	rna-XM_014664894.2	0.2244	1.1396	0.1969	912	201.8333	710.1667	137.7500	118.2500	0.1940	0.5859
rna-XM_014667673.2	rna-XM_022775923.1	0.1594	0.6126	0.2601	864	188.9167	675.0833	96.9167	79.0833	0.1436	0.4186
rna-XM_022782825.1	rna-XM_022775636.1	0.1127	0.9731	0.1159	1494	342.0000	1152.0000	120.5833	186.4167	0.1047	0.5451
rna-XM_022785228.1	rna-XM_022780152.1	0.3979	1.9011	0.2093	447	94.2500	352.7500	108.9167	65.0833	0.3088	0.6905

Table S3

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Fig. 10

Fig. 11

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登录号 Accession number	基因名称 Gene name	上游引物 Forward primer (5'-3')	下游引物 Reverse primer (5'-3')
XP_014495171.1	VrMYB39	AAACTTCAGGCTGTTCTTGGC	AGATTGGTGTGGAGTGGCATT
XP_014495344.2	VrMYB40	ATGGACCAACTATCTCCGACCT	TTCCTCACTCTGCATCTTCTTCA
XP_014497212.1	VrMYB50	GAAGAGTTGTAGACTGAGATGGTTG	CTGTTGTGAGATTGTACTTTGCG
XP_014500543.1	VrMYB52	GATGGTCAATGATTGCAGGAA	CGAGTCTTGTCACGAGAGGCT
XP_014505357.1	VrMYB68	GGAAGAGAGCCAAAGCACAGA	AGAAGAGGCAAGATAACCAGGAA
XP_014505359.1	VrMYB72	GCCACTTCACCCTTCACCTTA	TTCTCCAATCACCTTTCCCATA
XP_014508007.1	VrMYB79	TCTTTACCTTATCCCACCTCG	AGCAATTAATGGCTTCCACTG
XP_014505860.1	VrMYB84	ACGACGGTCCCACCACCT	ATTCAACGCTCCCATTCCAA
XP_014510156.1	VrMYB96	CTACTTCAAAGAGCGTCAGCAGT	GGTCAGAAATGTATTGGGAGAGC
XP_022638546.1	VrMYB98	AGTGACTGTGGGCTTGGACAC	TAGGACATTGCTGCTGCGTT
XP_014513300.1	VrMYB111	ACGGCAGGAGCAGTAGGGA	CCAAGAGTTCACGAAATCGGA
XP_022631357.1	VrMYB133	GTAACAACAAAAGCAGCGGTG	CAATGGTGGCAGATAATTCGTA
XP_014521508.1	VrMYB137	TACTTCCCTTTCCCTGTCCC	AACTCCTCACCTCCTTCCTTATC
XP_014491137.1	VrMYB156	CTGCTGCGGAATCTTCTCAC	ACTCCATCATCTCCTCCACTTG
NP_001304238.1	Actin	GTTCTGTTCCAGCCATCCAT	GTGGTGCGACAACCTTGATT

Identification of the R2R3-MYB transcription factor family and screening of genes regulating flavonoid synthesis in mung bean

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