谷子籽粒类黄酮含量和粒色的QTL定位

doi:10.3724/SP.J.1006.2024.34186

Abstract

Abstract:

Foxtail millet (Setaria italica L.) is an important grain crop in northern China. The grain is rich in nutrients and many kinds of flavonoid, which plays an important role in growth and quality formation. At present, there are few studies on the regulatory mechanisms of flavonoid synthesis and seed color formation in foxtail millet. The quantitative trait loci (QTL) analysis of flavonoid content and seed color traits in foxtail millet laid a foundation for fine mapping, cloning, and functional study of key genes for flavonoid synthesis, and it also provided technical support for revealing the mechanism of flavonoid synthesis and metabolism in foxtail millet and cultivating millet varieties rich in flavonoid. In this study, a recombinant inbred line (RIL) population consisting of 150 families was used as the experimental materials, which was constructed using a red seed-color high-flavonoid variety Jinmiaohongjiugu and a yellow seed-color low-flavonoid variety Yugu 28 as the parents. The related traits of seed color and flavonoid content were analyzed at maturity stage in foxtail millet. At the same time, composite interval mapping (CIM) was used to locate and analyze QTL for seed color and flavonoid content, and the candidate genes within the QTL confidence intervals were predicted. Correlation analysis showed that flavonoid content was significantly positively correlated with seed color. A total four and eleven QTL associated with flavonoids content and seed color were located on chromosomes 1, 2, 5, 6, 7, 8, and 9, respectively, and individual QTL phenotypic contribution rate was 2.01%-29.25%, six major QTLs were identified, qSC1-2 and qFLA1-1, qSC7-1 and qFLA7-1, qSC9-3, and qFLA9-1 were co-localized QTL under both two traits. Through gene prediction and functional annotation, five candidate genes related to flavonoid synthesis and metabolism in the confidence interval of QTL were screened, indicating that the genes related to flavonoid synthesis, metabolism, and utilization were most probably to regulate the expression of these genes. 15 QTL were clustered on seven chromosomes, respectively, and five candidate genes related to flavonoid synthesis and metabolism were screened based on gene functional annotation, indicating that different QTL loci were involved in the common genetic mechanism, and the pyramiding breeding of beneficial genes such as flavonoid synthesis and metabolism could be carried out through molecular marker-assisted selection.

Key words: foxtail millet, flavonoid content, seed colour, QTL mapping, candidate genes

QIN Na, YE Zhen-Yan, ZHU Can-Can, FU Sen-Jie, DAI Shu-Tao, SONG Ying-Hui, JING Ya, WANG Chun-Yi, LI Jun-Xia. QTL mapping for flavonoid content and seed color in foxtail millet[J].Acta Agronomica Sinica, 2024, 50(7): 1719-1727.

Figures/Tables 7

Table 1

Fig. 1

Table 2

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性状 Trait	年份 Year	亲本 Parents		RILs群体 RILs populations
性状 Trait	年份 Year	金苗红酒谷 Jinmiaohongjiugu	豫谷28 Yugu 28	范围 Range	平均值 Mean	标准差 SD
类黄酮	2021	10.39	5.38	4.07-11.37	6.92	0.60
Flavonoid (mg g^-1)	2022	10.28	5.46	4.12-10.48	6.78	0.62
粒色	2021	红粒 Red seed	黄粒 Yellow seed	0-2	0.80	0.11
Seed color	2022	红粒 Red seed	黄粒 Yellow seed	0-2	0.78	0.13

	类黄酮 Flavonoid		粒色 Seed color
	2021	2022	2021	2022
类黄酮Flavonoid
2021	1
2022	0.969^**	1
粒色 Seed color
2021	0.756^**	0.866^**	1
2022	0.832^**	0.841^**	0.987^**	1

QTL	染色体 Chr.	环境 Environment	标记区间 Markers interval	遗传位置 Genetic position (cM)	LOD值 LOD value	表型贡献率 Phenotypic variance efficiency (%)	加性效应 Additive effect
qSC1-1	1	BLUP (2021, 2022)	SICAAS1049-SICAAS1006	30.39-50.57	10.70	7.87	0.27
qSC1-2	1	BLUP (2021, 2022)	SICAAS1052-SICAAS1068	134.52-175.47	31.59	29.25	-0.78
qSC1-3	1	BLUP	SICAAS1068-SICAAS1008	175.47-192.79	6.04	9.08	0.43
qSC2-1	2	BLUP (2022)	SICAAS2035-SICAAS2038	0-35.40	6.45	5.93	0.34
qSC5-1	5	BLUP (2021, 2022)	SICAAS5034-SICAAS5035	40.16-59.87	2.89	2.01	-0.11
qSC6-1	6	BLUP (2022)	SICAAS6081-SICAAS6002	27.38-41.67	5.23	11.48	0.40
qSC7-1	7	BLUP	SICAAS7019-SICAAS7073	49.53-61.76	3.01	10.67	0.32
qSC8-1	8	BLUP	SICAAS8001-SICAAS8019	5.39-45.95	2.78	3.51	0.06
qSC9-1	9	BLUP (2021)	SICAAS9023-SICAAS9107	0-68.88	3.86	3.68	0.02
qSC9-2	9	BLUP (2022)	SICAAS9107-SICAAS9131	68.88-123.22	2.79	3.52	-0.01
qSC9-3	9	BLUP (2021, 2022)	SICAAS9131-SICAAS9042	123.33-169.79	2.94	3.51	0.03
qFLA1-1	1	BLUP (2021, 2022)	SICAAS1052-SICAAS1068	134.52-175.47	4.52	16.89	0.71
qFLA5-1	5	BLUP (2021, 2022)	SICAAS5046-SICAAS5008	133.79-153.33	2.70	10.87	0.60
qFLA7-1	7	BLUP (2022)	SICAAS7019-SICAAS7073	49.53-61.76	3.05	9.63	0.54
qFLA9-1	9	BLUP	SICAAS9131-SICAAS9042	123.33-169.79	3.56	18.05	0.62

QTL	染色体 Chr.	标记 Marker	候选基因 Candidate genes	功能注释 Functional annotation	基因物理位置 Gene physical location (Mb)
qSC1-2	1	SICAAS1052-SICAAS1068	Seita.1G240600	苯丙氨酸解氨酶 Phenylalanine ammonia-lyase	31,806,380-31,808,846
qFLA5-1	5	SICAAS5046-SICAAS5008	Seita.5G070500	异黄酮还原酶 Isoflavone reductase	6,041,130-6,042,851
qSC5-1	5	SICAAS5060-SICAAS5034	Seita.5G124200	UDP-糖基转移酶 UDP-glycosyltransferase	10,408,350-10,412,722
qSC8-1	8	SICAAS8001-SICAAS8019	Seita.8G188600	香豆酰辅酶A Agmatine coumaroyl CoA	33,585,009-33,586,364
qFLA9-1	9	SICAAS9131-SICAAS9042	Seita.9G169100	醛酮还原酶 Aldo/keto reductase	55,949,693-55,952,956

QTL mapping for flavonoid content and seed color in foxtail millet

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