Abstract:

Simian 3 is one of major cultivars at the cotton-growing district in Yangtze River Valley in the 1990s. New breeding materials or varieties can be rapidly developed through direct transformation of exogenous genes. In this study, we generated transgenic plants from hypocotyl segments of Simian 3 by Agrobacterium-mediated transformation. The rates of callus induction and differentiation were higher in Simian 3 than those in Coker 312. And the major callus morphological characters of Simian 3 from which differentiation center could be formed differed from Coker 312. Histochemical GUS (β-glucuronidase) assay and PCR for nptⅡ (neomycin phosphotransferase) gene were carried out with the transgenic embryogenic callus and leaves of transgenic plantlets. The plantlets proved by histochemical GUS assay and PCR for nptⅡ were grafted and then cultivated in greenhouse. The application of kanamycin at the level of 2 063.98 μmol L^-1 and Southern blot also proved that the target gene was successfully integrated into the genome of Simian 3.

Key words: Simian 3, Agrobacterium-mediated transformation, Regeneration