Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (3): 747-755.doi: 10.3724/SP.J.1006.2024.31032

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of cutting on the recovery characteristics, grain and straw yield, and quality traits of Qingke

HE Jia-Qi1(), BAI Yi-Xiong1,3,4, YAO Xiao-Hua1,4, YAO You-Hua1,4, AN Li-Kun1,4, WANG Yu-Qin2, WANG Xiao-Ping1, LI Xin1,4, CUI Yong-Mei1,4, WU Kun-Lun1,4,*()   

  1. 1Qinghai University / Qinghai Academy of Agriculture and Forestry Sciences / Qinghai Key Laboratory of Qingke Genetic Breeding / National Improvement Center for Cereal Crops, Qingke Branch in Qinghai Province, Xining 810016, Qinghai, China
    2College of Animal Science and Veterinary Medicine, Qinghai University (Qinghai Academy of Animal Science and Veterinary Medicine), Xining 810016, Qinghai, China
    3College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
    4Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining 810016, Qinghai, China
  • Received:2023-05-10 Accepted:2023-09-13 Online:2024-03-12 Published:2023-09-27
  • Contact: *E-mail: wklqaaf@126.com
  • Supported by:
    Science and Technology Achievement Transformation Project of Qinghai Province(2020-NK-119);Key Research and Development Project of Qinghai Academy of Agricultural and Forestry Sciences(2019-NKY-01);National Natural Science Foundation of China(32060483);China Agriculture Research System of MOF and MARA(CARS-05);Natural Science Foundation Project of Qinghai Province(1-5)

RichHTML421

12

PDF

166

Abstract:

The objective of this study is to investigate the changes in agronomic and physicochemical characteristics of Qingke plants during regeneration and recovery after cutting and to analyze the response patterns of grain and straw yield, as well as quality indicators of Qingke. The findings will provide a theoretical basis for the application of the “food-seed-forage trinity” production model of Qingke and the analysis of regulatory mechanisms for plant regeneration and recovery in Qingke. This experiment was conducted from 2021 to 2022, focused on the Qingke varieties Kunlun 18 and Zang 1257. Using normal growth (CK) as the control, a two-factor split-plot design was adopted. Samples were taken at seven developmental stages: 0 hour (T0), 2 hour (T1), 24 hours (T2), 72 hours (T3) after cutting, jointing stage (T4), heading stage (T5), and grain filling stage (T6), which aimed to analyze the regeneration rate of stem recovery and changes in antioxidant enzymes in the stubble after cutting and to analyze the yield, quality characteristics of Qingke straw and grain, and the rapid response patterns of endogenous hormones after cutting. The results indicated that cutting increased the regrowth of Qingke straw and grain yield. It also leaded to higher levels of crude protein and crude ash content in the stem, while reducing the fiber content. As a result, the relative feeding value of the straw was significantly improved. Cutting promoted the recovery rate of plant height and individual biomass growth in the stem of Qingke. It increased the number of spikes per hectare and 1000-grain weight, resulting in a significant increase in grain yield. Additionally, it leaded to the accumulation of higher levels of crude protein and starch in the grains. After cutting, the levels of superoxide dismutase (SOD) and catalase (CAT) in the stubble of Qingke increased rapidly. It also significantly increased the content of trans-zeatin riboside (TZR) and isopentenyladenosine (iPA) in the stubble. However, it leaded to a significant decrease in the levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) in the stubble. Cytokinins and auxins in the stubble of Qingke may play important regulatory roles in the rapid response of the antioxidant enzyme system and the rapid regeneration process of above-ground parts after cutting. This study provided the foundation for the analysis of the regulatory mechanisms in the post-cutting regeneration and recovery of Qingke plants.

Key words: regeneration recovery, yield, feed quality, antioxidant enzymes, endogenous hormones

Fig. 1

Effect of cutting on straw yield CK1: normal growth of Kunlun 18; M1: mowing treatment of Kunlun 18; CK2: normal growth of Tibetan 1257; M2: mowing treatment of Tibetan 1257. Different lowercase letters indicate significant differences at the 0.05 probability level in the same year."

Table 1

Effect of mowing treatment on quality characteristics of barley straw after stubble"

年份
Year		 处理
Treatment		 粗蛋白
Crude protein (%)		 粗灰分
Ash
(%)		 纤维素
Cellulose
(%)		 半纤维素
Hemicellulose (%)		 木质素
Lignin
(%)		 酸性洗涤纤维
Acid detergent fiber (%)		 中性洗涤纤维
Neutral
detergent fiber (%)		 相对饲喂价值
RFV
2021 CK1 4.09 e 8.62 cd 40.80 a 29.56 c 5.73 a 43.82 b 73.29 a 69.51 d
M1 5.13 b 9.69 a 38.46 bc 27.54 e 5.68 a 39.77 e 67.44 c 79.89 b
CK2 4.61 d 8.73 c 39.19 b 32.68 a 5.28 c 45.29 a 71.51 b 69.75 d
M2 5.53 a 9.82 a 37.22 de 30.82 b 5.17 c 41.08 d 63.48 e 83.38 a
2022 CK1 3.90 f 8.41 d 39.33 b 28.50 d 5.64 ab 42.55 c 69.83 b 74.27 c
M1 4.97 c 9.41 b 37.97 cd 26.26 f 5.54 b 39.59 e 65.71 d 80.96 b
CK2 4.59 d 8.56 cd 39.02 b 32.44 a 5.26 c 44.06 b 67.13 cd 75.63 c
M2 5.46 a 9.79 a 36.86 e 30.45 b 5.21 c 41.04 d 63.82 e 82.98 a

Table 2

Effect of mowing on seed yield"

年份
Year		 处理
Treatment		 穗粒数
Number of grains per ear		 千粒重
1000-grain weight (g)		 每公顷穗数
Number of spikes per hm-2 (×106)		 籽粒产量
Grain yield (t hm-2)
2021 CK1 53.77 ab 34.42 d 4.37 c 3.42 d
M1 51.09 cd 35.37 cd 4.43 a 3.59 a
CK2 51.67 cd 37.96 b 4.15 e 3.36 e
M2 49.26 e 39.36 a 4.22 d 3.48 c
2022 CK1 54.64 a 34.94 d 4.35 c 3.44 d
M1 52.61 bc 36.15 c 4.40 b 3.60 a
CK2 52.44 bc 38.21 ab 4.11 f 3.32 f
M2 50.46 de 39.22 a 4.17 e 3.54 b

Table 3

Effects of cutting on grain quality"

年份
Year		 处理
Treatment		 粗蛋白
Protein content (%)		 淀粉
Starch content (%)
2021 CK1 11.30 d 69.95 d
M1 11.67 b 75.15 a
CK2 11.50 f 72.80 e
M2 12.18 d 73.90 bc
2022 CK1 10.30 c 65.77 ab
M1 11.28 a 71.26 a
CK2 11.10 e 71.30 bc
M2 12.21 a 73.60 ab

Fig. 2

Effect of cutting on stem regeneration recovery characteristics in 2021 and 2022 Fig. a-c show data for 2021 and Fig. d-f show data for 2022. CK1: normal growth of Kunlun 18; M1: mowing treatment of Kunlun 18; CK2: normal growth of Tibetan 1257; M2: mowing treatment of Tibetan 1257. T1: 2 h; T2: 24 h; T3: 72 h; T4: jointing stage; T5: heading stage; T6: filling stage."

Fig. 3

Effect of mowing on stem protective enzyme activity in 2021 and 2022 Fig. a-c show data for 2021 and Fig. d-f show data for 2022. POD: peroxidase; SOD: superoxide dismutase; CAT: catalase. * and ** indicate significant differences at the 0.05 and 0.01 probability levels, respectively. Treatments and fertility periods are the same as those given in Fig. 2."

Fig. 4

Effect of mowing on endogenous hormones in the stem in 2021-2022 Fig. a-e show data for 2021 and Fig. f-j show data for 2022. TZR: trans-zeatin nucleoside; iPA: isopentenyl adenosine; tZT: trans-zeatin; IAA: indole-3-acetic acid; ABA: abscisic acid. * and ** indicate significant differences at the 0.05 and 0.01 probability levels, respectively. Treatments and fertility periods are the same as those given in Fig. 2."

Fig. 5

Correlation analysis of the relative values of each individual index Ph: plant height; Cs: culm strength; Mb: single plant biomass; TZR: trans-zeatin nucleoside; iPA: isopentenyl adenosine; tZT: trans-zeatin; IAA: indole-3-acetic acid; ABA: abscisic acid; POD: peroxidase activity; SOD: superoxide dismutase activity; CAT: catalase activity. * and ** indicate significant correlation at the 0.05 and 0.01 probability levels, respectively."

[1] 王燕, 姚晓华, 姚有华, 苏乐平, 安立昆, 吴昆仑. 青稞籽粒花青素合成相关基因HvnF3’M的克隆与表达分析. 西北农业学报, 2022, 31: 1174-1184.
Wang Y, Yao X H, Yao Y H, Su L P, An L K, Wu K L. Cloning and expression analysis of HvnF3’M gene related to anthocyanin synthesis of hulless barley. Acta Agric Boreali-occident Sin, 2022, 31: 1174-1184 (in Chinese with English abstract).
[2] 李洁. 干旱胁迫对青稞幼苗可溶性蛋白的影响. 江苏农业科学, 2015, 43(12): 124-126.
Li J. Effect of drought stress on soluble protein of barley seedlings. Jiangsu Agric Sci, 2015, 43(12): 124-126 (in Chinese with English abstract).
[3] Quiroga R E, Blanco L J, Namur P R. Defoliation intensity and simulated grazing strategy effects on three C4 rangeland bunchgrasses. Rangeland Ecol Manag, 2018, 71: 58-66.
doi: 10.1016/j.rama.2017.09.002
[4] Newell M T, Hayes R C. An initial investigation of forage production and feed quality of perennial wheat derivatives. Crop Past Sci, 2017, 68: 1141-1148.
doi: 10.1071/CP16405
[5] Younis S. Effect of seeding rates and mowing stages on growth, yield and quality of forage and seed of common vetch (Vicia sativa L.). Mesopot J Agric, 2018, 46: 219-232.
[6] Butchee J D, Edwards J T. Dual-purpose wheat grain yield as affected by growth habit and simulated grazing intensity. Crop Sci, 2013, 53: 1686-1692.
doi: 10.2135/cropsci2013.01.0033
[7] 王小萍, 白羿雄, 姚晓华, 姚有华, 李新, 安立昆, 吴昆仑. 刈割留茬高度对青稞饲草与籽粒产量及饲用品质的影响. 麦类作物学报, 2023, 43: 513-523.
Wang X P, Bai Y X, Yao X H, Yao Y H, Li X, An L K, Wu K L. Effect of mowing stubble height on forage and grain yield and forage quality characteristics of hulless barley. J Triticeae Crops, 2023, 43: 513-523 (in Chinese with English abstract).
[8] Zeleke K T. Effect of grazing time and intensity on growth and yield of spring wheat (Triticum aestivum L.). J Integr Agric, 2019, 18: 204-213.
[9] 张腾薇, 吕二锁, 刘志萍, 包文龙, 段俊杰, 云颖, 张霞, 韩磊, 孙娟娟. 留茬高度对全株大麦青贮品质的影响. 中国草地学报, 2022, 44(7): 87-92.
Zhang T W, Lyu E S, Liu Z P, Bao W L, Duan J J, Yun Y, Zhang X, Han L, Sun J J. Effects of stubble height on silage quality of whole barley. Chin J Grassland, 2022, 44(7): 87-92 (in Chinese with English abstract).
[10] 李变变, 张凤华, 赵亚光. 刈割高度对油莎豆氮代谢及产量和品质的影响. 草业学报, 2023, 32(2): 84-96.
doi: 10.11686/cyxb2022061
Li B B, Zhang F H, Zhao Y G. Effects of stubble height on nitrogen metabolism, yield and quality of Cyperus esculentus. Acta Pratac Sin, 2023, 32(2): 84-96 (in Chinese with English abstract).
[11] 陈晓东, 赵斌, 季昌好, 朱斌, 王瑞. 刈割期对多棱饲料大麦饲草及籽粒产量与品质的影响. 麦类作物学报, 2017, 37: 409-413.
Chen X D, Zhao B, Ji C H, Zhu B, Wang R. Effect of defoliation timing on the yield and quality of six-row barley forage and grain. J Triticeae Crops, 2017, 37: 409-413 (in Chinese with English abstract).
[12] 赵小红, 白羿雄, 王凯, 姚有华, 姚晓华, 吴昆仑. 种植密度对2个青稞品种抗倒伏及秸秆饲用特性的影响. 作物学报, 2020, 46: 586-595.
doi: 10.3724/SP.J.1006.2020.91038
Zhao X H, Bai Y X, Wang K, Yao Y H, Yao X H, Wu K L. Effects of planting density on lodging resistance and straw forage characteristics in two hulless barley varieties. Acta Agric Sin, 2020, 46: 586-595 (in Chinese with English abstract).
[13] Cui G, Zhao M, Zhang S, Wang Z, Meng M, Sun F, Zhang C, Xi Y. MicroRNA and regulation of auxin and cytokinin signaling during post-mowing regeneration of winter wheat (Triticum aestivum L.). Plant Physiol Biochem, 2020, 155: 769-779.
doi: 10.1016/j.plaphy.2020.08.032
[14] Pan X, Welti R, Wang X. Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nat Protoc, 2010, 5: 986-992.
doi: 10.1038/nprot.2010.37 pmid: 20448544
[15] Tian L H, Bell L W, Shen Y Y, Whish J P M. Dual-purpose use of winter wheat in western China: cutting time and nitrogen application effects on phenology, forage production, and grain yield. Crop Past Sci, 2012, 63: 520-528.
doi: 10.1071/CP12101
[16] Lyons S E, Ketterings Q M, Cherney D J R, Cherney J H, Godwin G S, Kilcer T F. Growth stage at harvest influences forage quality and yield of brown midrib forage sorghum. J Anim Sci, 2017, 95: 136-136.
doi: 10.2527/asasann.2017.274
[17] Zhang H, Zhao Y, Zhu J K. Thriving under stress: how plants balance growth and the stress response. Dev Cell, 2020, 55: 529-543.
doi: 10.1016/j.devcel.2020.10.012 pmid: 33290694
[18] 许能祥, 顾洪如, 董臣飞, 程云辉, 张文洁, 丁成龙. 刈割对不同品种褐色中脉饲用高粱饲草品质及农艺性状的影响. 草地学报, 2014, 22: 623-630.
doi: 10.11733/j.issn.1007-0435.2014.03.028
Xu N X, Gu H R, Dong C F, Cheng Y H, Zhang W J, Ding C L. Feed quality and agronomic traits of brown midrib (BMR) forage sorghum varieties. Acta Agrest Sin, 2014, 22: 623-630 (in Chinese with English abstract).
[19] Chen X D, Zhao B, Ji C H, Zhu B, Wang R. Breeding for purpose: Sole- and dual-use barley. Agron J, 2021, 113: 1758-1765.
doi: 10.1002/agj2.v113.2
[20] 张晓佩, 高承芳, 刘远, 李文杨, 陈鑫珠, 董晓宁. 刈割高度对多花黑麦草新品种产量和品质的影响. 热带作物学报, 2014, 35: 1695-1698.
Zhang X P, Gao C F, Liu Y, Li W Y, Chen X Z, Dong X N. Mowing height effects on the yield and quality of new varieties of Lolium multiflorum. Chin J Tropic Crops, 2014, 35: 1695-1698 (in Chinese with English abstract).
[21] 杨景宁. 播期和刈割时期对粮饲兼用冬小麦生产力形成的影响机制. 兰州大学博士学位论文, 甘肃兰州, 2021.
Yang J N. Effects of Sowing Date and Cutting Regime on Productivity of Dual-Purposed Winter Wheat. PhD Dissertation of Lanzhou University, Lanzhou, Gansu, China, 2021 (in Chinese with English abstract).
[22] Jiang M Y, Zhang J H. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot, 2002, 53: 2401-2410.
doi: 10.1093/jxb/erf090 pmid: 12432032
[23] Srivalli S, Khanna-Chopra R. Delayed wheat flag leaf senescence due to removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins. Plant Physiol Biochem, 2009, 47: 663-670.
doi: 10.1016/j.plaphy.2009.03.015
[24] An Y, Zhang S. Effects of mechanical damage and herbivore wounding on H2O2 metabolism and antioxidant enzyme activities in hybrid poplar leaves. J For Res, 2009, 20, 156-160.
doi: 10.1007/s11676-009-0027-x
[25] 郭安琪, 周瑞莲, 宋玉, 马会雷. 刈割后黑麦草生理保护作用对其补偿性生长的影响. 生态学报, 2018, 38: 3495-3503.
Guo A Q, Zhou R L, Song Y, Ma H L. Relationship between physiological protection mechanisms and the compensatory growth of Lolium perenne L. at different cutting treatment levels. Acta Ecol Sin, 2018, 38: 3495-3503 (in Chinese with English abstract).
[26] Rong C, Liu Y, Chang Z, Liu Z, Ding Y, Ding C. Cytokinin oxidase/dehydrogenase family genes exhibit functional divergence and overlap in rice growth and development, especially in control of tillering. J Exp Bot, 2022, 73: 3552-3568.
doi: 10.1093/jxb/erac088 pmid: 35247044
[27] Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H. Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J, 2006, 45: 1028-1036.
doi: 10.1111/j.1365-313X.2006.02656.x pmid: 16507092
[28] Wu L Y, Shang G D, Wang F X, Gao J, Wan M C, Xu Z G, Wang J W. Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration. Cell, 2022, 57: 526-542.
[1] LOU Fei, ZUO Yi-Ping, LI Meng, DAI Xin-Meng, WANG Jian, HAN Jin-Ling, WU Shu, LI Xiang-Ling, DUAN Hui-Jun. Effects of organic fertilizer substituting chemical fertilizer nitrogen on yield, quality, and nitrogen efficiency of waxy maize [J]. Acta Agronomica Sinica, 2024, 50(4): 1053-1064.
[2] ZHANG Zhen, ZHAO Jun-Ye, SHI Yu, ZHANG Yong-Li, YU Zhen-Wen. Effects of different sowing space on photosynthetic characteristics after anthesis and grain yield of wheat [J]. Acta Agronomica Sinica, 2024, 50(4): 981-990.
[3] YUE Hai-Wang, WEI Jian-Wei, LIU Peng-Cheng, CHEN Shu-Ping, BU Jun-Zhou. Comprehensive evaluation of maize hybrids in the mega-environments of Huanghuaihai plain based on GYT biplot analysis [J]. Acta Agronomica Sinica, 2024, 50(4): 836-856.
[4] LIU Cheng-Min, MEN Ya-Qi, QIN Du-Lin, YAN Xiao-Yu, ZHANG Le, MENG Hao, SU Xun-Ya, SUN Xue-Zhen, SONG Xian-Liang, MAO Li-Li. Effects of nitrogen application rate on cotton yield and nitrogen utilization under long-term straw return to the field [J]. Acta Agronomica Sinica, 2024, 50(4): 1043-1052.
[5] ZOU Jia-Qi, WANG Zhong-Lin, TAN Xian-Ming, CHEN Liao-Yuan, YANG Wen-Yu, YANG Feng. Estimation of maize grain yield under drought stress based on continuous wavelet transform [J]. Acta Agronomica Sinica, 2024, 50(4): 1030-1042.
[6] WU Xia-Yu, LI Pan, WEI Jin-Gui, FAN Hong, HE Wei, FAN Zhi-Long, HU Fa-Long, CHAI Qiang, YIN Wen. Effect of reduced irrigation and combined application of organic and chemical fertilizers on photosynthetic physiology, grain yield and quality of maize in northwestern irrigation areas [J]. Acta Agronomica Sinica, 2024, 50(4): 1065-1079.
[7] WEI Huan-He, ZHANG Xiang, ZHU Wang, GENG Xiao-Yu, MA Wei-Yi, ZUO Bo-Yuan, MENG Tian-Yao, GAO Ping-Lei, CHEN Ying-Long, XU Ke, DAI Qi-Gen. Effects of salinity stress on grain-filling characteristics and yield of rice [J]. Acta Agronomica Sinica, 2024, 50(3): 734-746.
[8] LI Bo-Yang, YE Yin, CHU Rui-Wen, JING Miao, ZHANG Sui-Qi, YAN Jia-Kun. Effects of biochar application on dry matter accumulation, transport, and distribution of foxtail millet and soil physicochemical properties [J]. Acta Agronomica Sinica, 2024, 50(3): 695-708.
[9] SHANG Yong-Pan, YU Ai-Zhong, WANG Yu-Long, WANG Peng-Fei, LI Yue, CHAI Jian, LYU Han-Qiang, YANG Xue-Hui, WANG Feng. Effects of green manure application methods on dry matter accumulation, distribution, and yield of maize in oasis irrigation area [J]. Acta Agronomica Sinica, 2024, 50(3): 686-694.
[10] KE Hui-Feng, SU Hong-Mei, SUN Zheng-Wen, GU Qi-Shen, YANG Jun, WANG Guo-Ning, XU Dong-Yong, WANG Hong-Zhe, WU Li-Qiang, ZHANG Yan, ZHANG Gui-Yin, MA Zhi-Ying, WANG Xing-Fen. Identification for yield and fiber quality traits and evaluation of molecular markers in modern cotton varieties [J]. Acta Agronomica Sinica, 2024, 50(2): 280-293.
[11] LI Zhi-Kun, JIA Wen-Hua, ZHU Wei, LIU Wei, MA Zong-Bin. Effects of nitrogen fertilizer and DPC combined application on temporal distribution of cotton yield and fiber quality [J]. Acta Agronomica Sinica, 2024, 50(2): 514-528.
[12] XU Ran, YANG Wen-Ye, ZHU Jun-Lin, CHEN Song, XU Chun-Mei, LIU Yuan-Hui, ZHANG Xiu-Fu, WANG Dan-Ying, CHU Guang. Effects of different irrigation regimes on grain yield and water use efficiency in japonica-indica hybrid rice cultivar Yongyou 1540 [J]. Acta Agronomica Sinica, 2024, 50(2): 425-439.
[13] NIE Xiao-Yu, LI Zhen, WANG Tian-Yao, ZHOU Yuan-Wei, XU Zheng-Hua, WANG Jing, WANG Bo, KUAI Jie, ZHOU Guang-Sheng. Effect of planting density and weak light stress at pod-filling stage on seed oil accumulation in rapeseed [J]. Acta Agronomica Sinica, 2024, 50(2): 493-505.
[14] XIE Wei, HE Peng, MA Hong-Liang, LEI Fang, HUANG Xiu-Lan, FAN Gao-Qiong, YANG Hong-Kun. Effects of straw mulching from autumn fallow and phosphorus application on nitrogen uptake and utilization of winter wheat [J]. Acta Agronomica Sinica, 2024, 50(2): 440-450.
[15] WU Hao, ZHANG Ying, WANG Chen, GU Han-Zhu, ZHOU Tian-Yang, ZHANG Wei-Yang, GU Jun-Fei, LIU Li-Jun, YANG Jian-Chang, ZHANG Hao. Effects of cultivation optimization on root characteristics and starch properties of rice at grain filling stage in the lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2024, 50(2): 478-492.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Li Shaoqing, Li Yangsheng, Wu Fushun, Liao Jianglin, Li Damo. Optimum Fertilization and Its Corresponding Mechanism under Complete Submergence at Booting Stage in Rice[J]. Acta Agronomica Sinica, 2002, 28(01): 115 -120 .
[2] Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[3] YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[4] Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[5] Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[6] WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[7] TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[8] HU Xi-Yuan;LI Jian-Ping;SONG Xi-Fang. Efficiency of Spatial Statistical Analysis in Superior Genotype Selection of Plant Breeding[J]. Acta Agron Sin, 2008, 34(03): 412 -417 .
[9] WANG Yan;QIU Li-Ming;XIE Wen-Juan;HUANG Wei;YE Feng;ZHANG Fu-Chun;MA Ji. Cold Tolerance of Transgenic Tobacco Carrying Gene Encoding Insect Antifreeze Protein[J]. Acta Agron Sin, 2008, 34(03): 397 -402 .
[10] ZHENG Xi;WU Jian-Guo;LOU Xiang-Yang;XU Hai-Ming;SHI Chun-Hai. Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments[J]. Acta Agron Sin, 2008, 34(03): 369 -375 .
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd