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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (06): 939-942.

• RESEARCH NOTES • Previous Articles     Next Articles

PCR Detection of Nematode Resistant Gene in Sugarcane

WU Yang,ZHOU Hui, PAN Da-Ren   

  1. Crop College, Fujian Agriculture & Forestry University, Fuzhou 350002, Fujian, China
  • Received:2005-03-24 Revised:2005-12-15 Online:2006-06-12 Published:2006-06-12
  • Contact: PAN Da-Ren

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Abstract:

According to the sequence of root-knot nematode resistant gene in tomato and the sequence of nematode resistant gene in sugar beet, two forward primers and two reverse primers were separately designed, and a pair of primers each was selected among them by PCR. Thirty-eight sugarcane cultivars (without conventional identification of nematode resistance) was tested by the selected PCR primers. The results showed that two special fragments of about 460 bp and 690 bp were obtained, and PCR-Southern blotting about them was made further in order to affirm the homogeneous sequences of nematode resistant gene.

Key words: Sugarcane, Nematode resistant gene, Gene detection, PCR-Southern blotting

CLC Number: 

  • S566
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