Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 816-820.doi: 10.3724/SP.J.1006.2009.00816

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transferability of SSR from Adzuki Bean to Mungbean

WANG Li-Xie,CHENG Xu-Zhen*,WANG Su-Hua,LIU Chang-You,LIANG Hui   

  1. Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-08-08 Revised:2008-12-15 Online:2009-05-12 Published:2009-02-13
  • Contact: CHENG Xu-Zhen E-mail:chengxz@caas.net.cn

PDF

1762

Cited

10

Abstract:

SSR (Simple sequence repeat) has been a popular tool in genetic studies. However, only a few SSR markers are available in mungbean (Vigna radiata L.). Adzuki bean (Vigna angularis L.) is a close relative of mungbean. In the study reported in this paper, we tested the transferability of adzuki bean SSR markers into mungbean. A total of 187 sets of SSR primers were tested and about 75% of them generated repeatable and clear products in mungbean, indicating a high homology between these two genomes. Of the transferable SSRs, 28 sets of primers generated polymorphic products among 60 mungbean genotypes with a total of 81 alleles detected. The average PIC (polymorphic information content) value is 0.36. Based on the SSR fingerprints, the 60 accessions can be divided into different groups agreeing well with their geographical origins. The exotic accessions were well separated from the local genotypes, indicating the former could be important sources for broadening the mungbean gene pool. The present study identified useful SSR markers for mungbean research, which will undoubtedly accelerate the genetic study of this crop.

Key words: Adzuki bean, Mungbean, SSR, Transferability

[1] Zheng Z-J(郑卓杰), Wang S-M(王述民), Zong X-X(宗绪晓). Chinese Legumes (中国食用豆类学). Beijing: China Agriculture Press, 1997(in Chinese)
[2] Wang L X, Guan R X, Liu Z X, Chang R Z, Qiu L J. Genetic diversity of Chinese cultivated soybean revealed by SSR markers. Crop Sci, 2006, 46: 1032–1038
[3] R?der M S, Plaschke J, Konig S U, Borner A, Sorrells M E, Tanksley S D, Ganal M W. Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet, 1995, 246: 327–333
[4] Sun L, Wang C, Su C, Liu Y, Zhai H, Wan J. Mapping and marker-assisted selection of a brown plant hopper resistance gene bph2 in rice (Oryza sativa L.). Acta Genet Sin, 2006, 33: 717–723
[5] Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata. Mol Ecol Notes, 2002, 2: 293–295
[6] Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation of microsatellite markers in mungbean, Vigna radiata. Mol Ecol Notes, 2002, 2: 96–98
[7] Miyagi M, Humphry M, Ma Z Y, Lambrides C J, Bateson M, Liu C J. Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean. Theor Appl Genet, 2004, 110: 151–156
[8] Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A, Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean (Vigna radiata L). Mol Ecol Notes, 2006, 6: 1132–1134
[9] Liu C-Y(刘长友), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Sun L(孙蕾), Mei L(梅丽), Xu N(徐宁). The screening of SSR and STS markers for genetic diversity analysis of mungbean. J Plant Genet Res (植物遗传资源学报), 2007, 8(3): 298–302(in Chinese with English abstract)
[10] Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for azuki bean (Vigna angularis Willd. Ohwi & Ohashi). Theor Appl Genet, 2005, 111: 1278–1287
[11] Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the Genus and other legume genera: Implications for the transferability of SSRs in plants. Mol Biol Evol, 1998, 15: 1275–1287
[12] Sharma R K, Gupta P, Sharma V, Sood A, Mohapatra T, Ahuja P S. Evaluation of rice and sugarcane SSR markers for phylogenetic and genetic diversity analyses in bamboo. Genome, 2008, 51: 91–103
[13] Rohlf FJ. NTSYS-pc2.1 numerical taxonomy and multivariate analysis system. New York: State University of New York, 1992
[14] Bisht I S, Mahajan R K, Patel D P. The use of characterisation data to establish the Indian mungbean core collection and assessment of genetic diversity. Genet Res Crop Evol, 1998, 45: 127–133
[15] Han F-X(韩粉霞), Li G-Y(李桂英). Correlation analysis between main agronomical traits of mungbean. North China J Agric Sci (华北农学报), 1998, 13(4): 66–69(in Chinese with English abstract)
[16] Bhattacharya A, Vijaylaxmi. Genetic diversity in mungbean: Phenological, physiological and yield forming traits. Legume Res, 2005, 28: 1–6
[17] Chattopadhyay K, Ali M N, Sarkar H K, Mandal N, Bhattacharyya S. Diversity analysis by RAPD and ISSR markers among the selected mungbean(Vigna radiata L. Wilczek) genotypes. Indian J Genet Plant Breed, 2005, 65: 173–175
[18] Chen X(陈新), Yan J-Y(严继勇), Gao B(高兵), Zhang Z-M(张智民), Shrivies P. The evaluation on traits of F1 plants between diverse parents. Jiangsu Agric Sci (江苏农业科学), 2006, (5): 34–35(in Chinese)
[19] Cheng X-Z(程须珍), Yang C Y. Studies on relationships among species in mungbean groups using RAPD markers. Sci Agric Sin (中国农业科学), 2001, 34(2): 216–218(in Chinese with English abstract)
[20] Afzal M A, Haque M M, Shanmugasundatam S. Random amplified polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek) cultivars. Asian J Plant Sci, 2004, 3: 20–24
[21] Ma L-P(马丽萍), Cheng X-Z(程须珍), Zhang H(张辉). Study on AFLP marker related to bruchid resistance gene in wild mungbean germplasm TC1966. Southwest China J Agric Sci (西南农业学报), 2005, 8(5): 629–633(in Chinese with English abstract)
[22] Cheng X Z, Wang S H, Wu S Y, Zhou J H, Wang S M, Yang C Y. Tagging and utilization bruchid resistance gene using PCR markers in mungbean. Agric Sci China, 2005, 4: 579–583
[23] Selvi R, Muthiah A R, Manivannan N, Raveendran T S, Manickam A, Samiyappan R. Tagging of RAPD marker for MYMV resistance in mungbean (Vigna radiata L. Wilczek). Asian J Plant Sci, 2006, 5: 277–280
[24] Soehendi R, Chanprame S, Toojinda T, Srinives P. Inheritance and AFLP tagging of leaflet mutants in mungbean (Vigna radiate L. Wilczek). Kasetsart J, 2006, 40: 566–572
[25] Sun L(孙蕾), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Liu C-Y(刘长友), Mei L(梅丽), Xu N(徐宁). Heredity analysis and gene mapping of bruchid resistance of a mungbean cultivar V2709. Sci Agric Sin (中国农业科学), 2008, 41(5): 1297–1307(in Chinese with English abstract)
[26] Wang L, Guan Y, Guan R, Li Y, Ma Y, Dong Z, Liu X, Zhang H, Zhang Y, Liu Z, Chang R, Xu H, Li L, Lin F, Luan W, Yan Z, Ning X, Zhu L, Cui Y, Piao R, Liu Y, Chen P, Qiu L. Establishment of Chinese soybean (Glycine max) core collections with agronomic traits and SSR markers. Euphytica, 2006, 151: 215–223
[1] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[2] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[3] HU Liang-Liang, WANG Su-Hua, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage [J]. Acta Agronomica Sinica, 2022, 48(2): 367-379.
[4] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[5] WU Ran-Ran, LIN Yun, CHEN Jing-Bin, XUE Chen-Chen, YUAN Xing-Xing, YAN Qiang, GAO Ying, LI Ling-Hui, ZHANG Qin-Xue, CHEN Xin. Genetic and cytological analysis of male sterile mutant msm2015-1 in mungbean [J]. Acta Agronomica Sinica, 2021, 47(5): 860-868.
[6] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[7] XIANG Hong-Tao, LI Wan, ZHENG Dian-Feng, WANG Shi-Ya, HE Ning, WANG Man-Li, YANG Chun-Jie. Effects of uniconazole and waterlogging stress in seedling stage on the physio logy and yield in adzuki bean [J]. Acta Agronomica Sinica, 2021, 47(3): 494-506.
[8] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[9] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[10] WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[11] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[12] Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[13] LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F2 population in Chinese pea [J]. Acta Agronomica Sinica, 2020, 46(10): 1496-1506.
[14] YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[15] Ning HE,Xue-Yang WANG,Liang-Zi CAO,Da-Wei CAO,Yu LUO,Lian-Zi JIANG,Ying MENG,Chun-Xu LENG,Xiao-Dong TANG,Yi-Dan LI,Shu-Ming WAN,Huan LU,Xu-Zhen CHENG. Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings [J]. Acta Agronomica Sinica, 2019, 45(3): 460-468.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd