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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 768-777.doi: 10.3724/SP.J.1006.2009.00768

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Expression Profile and Main Function Genes during Ear Development in a Highly Heterotic Hybrid of Maize

LI Bo,ZHANG Deng-Feng,JIA Guan-Qing,ZHANG Ti-Fu,DAI Jing-Rri,WANG Shou-Cai*   

  1. National Maize Improvement Center of China/Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry/Key Laboratory of Genetic Improvement of Beijing City;China Agricultural University,Beijing 100193,China
  • Received:2008-09-16 Revised:2009-02-15 Online:2009-05-12 Published:2009-03-20
  • Contact: WANG Shuo-Cai,010-62732406 E-mail:wangsc@cau.edu.cn

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Abstract:

The development of ears is mostly responsible for the yield of maize (Zea mays L.), however, the molecular basis is unclear. To disclose the differential expression genes involved in the development of maize ear and their expression patterns, the gene expression at genome level of a highly heterotic hybrid (C8605-2×W1445) was detected from ear spikelet differentiation initiation to the later 8 d using microarrays with approximately 58 000 probes. The result of microarray analysis was verified using quantitative real-time PCR. A total of 671 genes expressed differentially which consisted of four expression patterns. These genes were involved in several biological processes, such as metabolism, development, responses to stimulus, cell signal transduction, and transport. Most genes for cell division, cell wall structure, and the modified protein of cell wall structure were identified to be upregulated during the ear development. Thus, these genes may play important roles in cell differentiation and formation of agronomic traits in maize ears. Moreover, genes for protein kinase, signal transduction, and transcription factors, which are involved in signal transduction and regulatory processes, may also take great functions in the development of maize ears.

Key words: Microarray, Gene expression, Ear, Development, Maize


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