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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2029-2036.doi: 10.3724/SP.J.1006.2009.02029

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression of Wheat Transcription Factor Gene TaWRKY72b-1 and Its Effect on Phosphorus Use Efficiency in Transgenic Tobacco Plants

MIAO Hong-Ying1,ZHAO Jin-Feng1,LI Xiao-Juan2,SUN Zhao-Hua2,LU Wen-Jing2,GU Jun-Tao2,GUO Cheng-Jin1,XIAO Kai1   

  1. 1 College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2 College of Life Science, Agricultural University of Hebei, Baoding 071001, China
  • Received:2008-12-22 Revised:2009-04-25 Online:2009-11-12 Published:2009-08-07
  • Contact: XIAO Kai, E-mail: xiaokai@hebau.edu.cn

Abstract:

Phosphorus is one of the indispensable elements in plant growth and development, which is the main component for ATP, nucleic acid, and lecithin. Since phosphorus usually exists in the hard-absorption compounds in soil to cause plant suffering from phosphorus deficiency during growing stage. So plant morphological and physiologically develops the adaptation to low phosphorus stress. Transcription regulation plays an important role in responding to deficient-P cue in plants. Several transcription factors mediating the deficient-P signal transduction have been reported in Arabidopsis and rice. But no similar studieshave been conducted in wheat by now. In this study, an expressed sequence tag (EST) homologous to Arabidopsis WRKY75 was identified based on sequencing of clones from a subtractive root cDNA library, in which the differential expressed genes responding to low-P were enriched. The EST gene with high similarity to wheat WRKY72b (GenBank accession No. EF368383), was cloned and referred to TaWRKY72b-1. TaWRKY72b-1 had two base differences with WRKY72b at the cDNA sequence, with an open reading frame (ORF) of 621 bp and encoding a polypeptide of 206 amino acids. TaWRKY72b-1 contained one of conserved WRKY motif and one of C2H2 zinc finger motif. Phylogenetic tree analysis indicated that TaWRKY72b-1, wheat WRKY72a and barley WRKY12  werepossibly derived from one ancestor. Compared with those in sufficient-P (2 mmol L-1 P) condition, the transcripts of TaWRKY72b-1 in roots and leaves under the deficient-P (20 µmol L-1 P) condition were all dramatically increased, suggesting that TaWRKY72b-1 gene was involved in the response to low P stress in the plants. Under deficient-P condition, the expression of TaWRKY72b-1 in transgenic tobacco plants obviously increased plant dry weights, plant accumulative phosphorus amount, and phosphorus utilization efficiency compared with that in CK (empty vector transformed plants). Therefore, the TaWRKY72b-1 gene has possibly a potential use in improving the crop phosphorus use efficiency under low-Pi stress condition.

Key words: Wheat(Triticum aestivum L.), WRKY transcription factor, Gene cloning, Expression, Function, Low phosphorus stress

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