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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 442-448.doi: 10.3724/SP.J.1006.2010.00442


Analsysis of QTLs for Root Traits at Seedling Stage Using an "Immortalized F2"Population in Wheat

LI Zhuo-Kun1,PENG Tao1,2,ZHANG Wei-Dong1,XIE Quan-Gang1,TIAN Ji-Chun1,*   

  1. 1 Group of Quality Wheat Breeding of Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; 2 Institute of Jiyuan Agricultural Science, Jiyuan 454652, China
  • Received:2009-07-07 Revised:2009-10-03 Online:2010-03-12 Published:2010-01-22
  • Contact: TIAN Ji-Chun,E-mail:jctian@sdau.edu.cn;Tel:0538-8242040


Identification of quantitative trait loci (QTLs) of roots plays an important role in the genetic mechanism study of root system. A number of QTLs for root traits have been detected using different populations in rice (Oryza sativa L.), but rarely in wheat (Triticum aestivum L.). The “immortalized F2” population is favorable for QTL analysis due to their similar proportions of genotypes in the population, and the replicability at multiple locations over several years. The objective of this study was to detect QTLs for several root traits at seedling stage in wheat. From a set of doubled haploid lines derived from Huapei 3 × Yumai 57, an “immortalized F2” population was constructed with 168 single crosses. Wheat seedlings were hydroponically cultured with Hoagland’s solution in a light incubator, and sampled when the fifth leaf emerged. Nine root traits including root total length, root surface area, root average diameter, root volume, root tips, maximum root length, shoot dry weight, root dry weight, and ratio of root dry weight to shoot dry weight per plant were measured using the WinRHIZO Root Analysis System. QTLs associated with the nine traits were detected using composite interval mapping (CIM) method. A total of seven additive QTLs and twelve pairs of epistatic QTLs were mapped on chromosomes 1A, 1D, 2A, 2B, 2D, 3A, 3B, 5D, 6D, and 7D. Additive, dominant and epistatic effects were observed across these QTLs, including the interactions between additive and additive, additive and dominance, dominance and additive, as well as dominance and dominance. The phenotypic variation explained by each QTL ranged from 0.01% to 11.91%. In the interval between XWMC41 and XBARC349.2 on chromosome 2D, a QTL for total root length and the root dry weight was detected. Epistasis was of great importance in root growth and development at seedling stage in wheat. Theresults showed a complex mechanism on the genetics of root traits in wheat, and the eight root traits were significantly correlated with each other. In marker-assisted breeding practice, several root traits should be considered simultaneously for an integrated performance of roots with proper size and shape.

Key words: Common wheat, Immortalized F2 population, Root traits, Quantitative trait locus mapping, Gene action

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