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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 442-448.doi: 10.3724/SP.J.1006.2010.00442

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analsysis of QTLs for Root Traits at Seedling Stage Using an "Immortalized F2"Population in Wheat

LI Zhuo-Kun1,PENG Tao1,2,ZHANG Wei-Dong1,XIE Quan-Gang1,TIAN Ji-Chun1,*   

  1. 1 Group of Quality Wheat Breeding of Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; 2 Institute of Jiyuan Agricultural Science, Jiyuan 454652, China
  • Received:2009-07-07 Revised:2009-10-03 Online:2010-03-12 Published:2010-01-22
  • Contact: TIAN Ji-Chun,E-mail:jctian@sdau.edu.cn;Tel:0538-8242040

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Abstract:

Identification of quantitative trait loci (QTLs) of roots plays an important role in the genetic mechanism study of root system. A number of QTLs for root traits have been detected using different populations in rice (Oryza sativa L.), but rarely in wheat (Triticum aestivum L.). The “immortalized F2” population is favorable for QTL analysis due to their similar proportions of genotypes in the population, and the replicability at multiple locations over several years. The objective of this study was to detect QTLs for several root traits at seedling stage in wheat. From a set of doubled haploid lines derived from Huapei 3 × Yumai 57, an “immortalized F2” population was constructed with 168 single crosses. Wheat seedlings were hydroponically cultured with Hoagland’s solution in a light incubator, and sampled when the fifth leaf emerged. Nine root traits including root total length, root surface area, root average diameter, root volume, root tips, maximum root length, shoot dry weight, root dry weight, and ratio of root dry weight to shoot dry weight per plant were measured using the WinRHIZO Root Analysis System. QTLs associated with the nine traits were detected using composite interval mapping (CIM) method. A total of seven additive QTLs and twelve pairs of epistatic QTLs were mapped on chromosomes 1A, 1D, 2A, 2B, 2D, 3A, 3B, 5D, 6D, and 7D. Additive, dominant and epistatic effects were observed across these QTLs, including the interactions between additive and additive, additive and dominance, dominance and additive, as well as dominance and dominance. The phenotypic variation explained by each QTL ranged from 0.01% to 11.91%. In the interval between XWMC41 and XBARC349.2 on chromosome 2D, a QTL for total root length and the root dry weight was detected. Epistasis was of great importance in root growth and development at seedling stage in wheat. Theresults showed a complex mechanism on the genetics of root traits in wheat, and the eight root traits were significantly correlated with each other. In marker-assisted breeding practice, several root traits should be considered simultaneously for an integrated performance of roots with proper size and shape.

Key words: Common wheat, Immortalized F2 population, Root traits, Quantitative trait locus mapping, Gene action

[1] Liu T-J(刘桃菊), Qi C-H(戚昌瀚), Tang J-J(唐建军). Studies on relationship between the character parameters of root and yield formation in rice. Sci Agric Sin (中国农业科学), 2002, 35(11): 1416-1419 (in Chinese with English abstract)

[2] Partha D A S, Ali M N, Sarkar H K. Genetical studies on roots in bread wheat. J Interacademicia, 2004, 8: 166-168

[3] Moudal S K, Kour K. Genetic variability and correlation coefficients of some root characteristics and yield components in bread wheat (Triticum aestivum L.) under rainfed condition. Environ Ecol, 2004, 22: 646-648

[4] Caradus J R. Genetic control of phosphorus uptake and phosphorus status in plants. In: Genetic Manipulation of Crop Plants to Enhance Integrated Nutrient Management in Cropping System. Patancheru, India: ICRISAT Asia Centre, 1995. pp 55-74

[5] Champoux M C, Wang G, Sarkarung S, Mackill D J, Toole T C O, Huang N, McCouch S R. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet, 1995, 90: 969-981
[6] Ray J D, Yu L X, Mccouch S R, Mackill D J, Toole T C O, Huang N, MeCouch S R. Mapping quantitative trait loci associated with root penetration ability in rice (Oryza sativa L.). Theor Appl Genet, 1996, 92: 627-636
[7] Fang P(方萍), Wu P(吴平), Tao Q-N(陶勤南). QTLs for rice root morphological characters. Acta Agron Sin (作物学报), 1999, 25(2): 181-185 (in Chinese with English abstract)

[8] Mu P(穆平), Li Z-C(李自超), Li C-P(李春平), Zhang H-L(张洪亮), Wu C-M(吴长明), Li C(李晨), Wang X-K(王象坤). QTL mapping and G×E interaction for root traits in a DH population from japonica upland and lowland rice cross under three ecosystems. Chin Sci Bull (科学通报), 2003, 48(20): 2162-2169 (in Chinese with English abstract)
[9] Zhang Z-B(张正斌), Xu P (徐萍). Reviewed on wheat genome. Hereditas (遗传), 2002, 24(3): 389-394 (in Chinese with English abstract)
[10] Zhou X-G(周晓果), Jing R-L(景蕊莲), Hao Z-F(郝转芳), Chang X-P(昌小平), Zhang Z-B(张正斌). Mapping QTL for seedling root traits in common wheat. Sci Agric Sin (中国农业科学), 2005, 38(10): 1951-1957 (in Chinese with English abstract)
[11] Hua J P, Xing Y Z, Wu W R, Xu C G, Sun X L, Yu S B, Zhang Q F. Single-locus heterotic effects and dominance by dominance interaction can adequately explain the genetic basis of heterosis in an elite hybrid. Proc Natl Acad Sci USA, 2003, 100: 2574-2579

[12] Hai Y(海燕), Kang M-H(康明辉). Breeding of a new wheat variety Huapei 3 with high yield and early maturing. Henan Agric Sci (河南农业科学), 2007, (5): 36-37 (in Chinese with English abstract)

[13] Guo C-Q(郭春强), Bai Z-A(柏志安), Liao P-A(廖平安), Jin W-K(靳文奎). New high quality and yield wheat variety Yumai 57. China Seed (中国种业), 2004, (4): 54 (in Chinese with English abstract)
[14] An D G, Su J Y, Liu Q Y, Zhu Y G, Tong Y P, Li J M, Jing R L, Li B, Li Z S. Mapping QTLs for nitrogen uptake in relation to the early growth of wheat (Triticum aestivum L.). Plant Soil, 2006, 284: 73-84
[15] Zhang K P, Tian J C, Zhao L, Liu B, Chen G F. Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars. Genetica, 2009, 135:257-265

[16] Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255-1264

[17] Yang J, Zhu J. Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet, 2005, 110: 1268-1274

[18] McIntosh RA, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Anderson O D. Catalogue of gene symbols for wheat. 2005
[2009-06-30].http://wheat.pw.usda.gov/ggpages/wgc/2005upd.html
[19] Li Z K, Luo L J, Mei H W, Shu Q Y, Tabien R, Zhong D B, Ying C S, Stansel J W, Khush G S, Paterson A H. Overdominance epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice: I. Biomass and grain yield. Genetics, 2001, 158: 1737-1753

[20] Mei H W, Li Z K, Shu Q Y, Guo L B, Wang Y P, Yu X Q, Ying C S, Luo L J. Gene actions of QTL affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross population. Theor Appl Genet, 2005, 110: 649-659
[21] Xing Y Z, Tan Y F, Hua J P, Sun X L, Xu C G, Zhang Q F. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor Appl Genet, 2002, 105: 248-257

[22] Yu S B, Li J X, Xu C G, Tan Y F, Gao Y J, Li H X, Zhang Q F, Saghai Maroof M A. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA, 1997, 94: 9226-9231

[23] Xu J-L(徐建龙), Xue Q-Z(薛庆中), Luo L-J(罗利军), Li Z-K(黎志康). QTL dissection of panicle number per plant and spikelet number per panicle in rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2001, 28(8): 752-759 (in Chinese with English abstract)
[24] Zhang K-P(张坤普), Xu X-B(徐宪斌), Tian J-C(田纪春). QTL mapping for grain yield and spike related traits in common wheat. Acta Agron Sin (作物学报), 2009, 35(2): 270-278 (in Chinese with English abstract)
[25] Rebetzke G J, Bruce S E, Kirkegaard J A. Longer coleoptiles improve emergence through crop residues to increase seedling number and biomass in wheat (Triticum aestivum L.). Plant Soil, 2005, 272: 87-100
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