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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 771-778.doi: 10.3724/SP.J.1006.2010.00771


Analysis of Plant Height Heterosis Based on QTL Mapping in Wheat

LI Zhuo-Kun1,XIE Quan-Gang1,ZHU Zhan-Ling1,LIU Jin-Liang2,HAN Shu-Xiao1,TIAN Bin1,YUAN Qian-Qian1,TIAN Ji-Chun1*   

  1. 1Croup of Quality Wheat Breeding of Key Laboratory of Crop Biology,Shandong Agricultural University,Tai'an 271018,China;2Agro-technique Extension station,Wenyang Town,Feicheng 271600,China
  • Received:2009-11-04 Revised:2010-01-06 Online:2010-05-12 Published:2010-03-15
  • Contact: TIAN Ji-Chun,E-mail:jctian@sdau.edu.cn,Tel:0538-8242040 E-mail:lizk1984@163.com, Tel: 0538-8248196-3


Plant height (PH) is an important indicator for plant type, population size, biomass, and resistance to lodging. Besides, it is also associated with grain yield in cereal crops. Thus, it is a constant focus in genetics and heterosis of wheat (Triticum aestivum L.). This study aimed at studying the molecular genetic basis of plant height heterosis in wheat. From a set of doubled haploid (DH) lines derived from Huapei 3 × Yumai 57, an “immortalized F2” population was constructed with 168 single crosses. The DH lines, IF2 population, and the parents were evaluated for plant height in three environments, i.e., in Tai’an, Shandong Province, in 2007 and 2008 cropping seasons and in Jiyuan, Henan Province, in 2008 cropping season. Based on the genetic map of quantitative trait locus (QTL) constructed in our previous study, we analyzed the heterosis of PH of wheat using the composite interval mapping method. A total of three additive QTLs, two dominance QTLs, four pairs of epistatic QTLs (including additive × additive, additive × dominance, dominance × additive, dominance × dominance), and 20 heterotic loci were detected for PH in the three environments. Two QTLs, Qph2D and Qph4D, were detected on chromosomes 2D and 4D with minor interaction of additive by environment. In addition, several other heterotic loci for PH were also identified on chromosome 2D at very close regions with similar marker intervals. Of them, QTLs Qph2D-2 and Qph2D-7 explained the variance of PH by 29.77% and 55.77%, respectively. Another QTL associated with PH heterosis, Qph7D-2, was mapped in the marker interval between Xwmc273.2 and Xcfd175 on chromosome 7D in the three environments. The results indicated that a few QTLs on chromosomes 2D, 4D, and 7D play an important role in PH heterosis in wheat. These loci have potential use for the improvement of PH in wheat breeding assisted with molecular markers.

Key words: Wheat, Immortalized F2 population, Plant height, Quantitative trait locus, Heterotic loci

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