绿豆遗传连锁图谱的整合

doi:10.3724/SP.J.1006.2010.00932

Abstract

Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. One genetic linkage map of mungbean (Vigna radiata, 2n=2x=22) for the recombinant inbred line population (RIL) derived from an intersubspecific cross between a highly bruchid-susceptible cultivar Berken and a highly bruchid-resistant wild type ACC41 (V. radiata subsp. sublobata) was established. A total of 103 polymorphic SSR markers were screened from 701 pairs markers for density-enhancement of the previous map. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 178 markers, including 96 SSR among which 90 locus from mungbean closely related species, 76 RFLP, four RAPD and two STS, spanning 12 linkage groups, covering total length 1 822.9 cM of the mungbean genome, with an average marker interval distance of 10.30 cM. In the study, we determined the SSR markers transferability of close relatives of mungbean including adzuki bean (Vigna angularis L.), black gram (Vigna mungo L.), common bean(Phaseolus vulgaris), cowpea (V. unguiculata) into mungbean genome. A total of 597 pairs SSR primers were tested and about 65%, 72%, 42%, 30% SSR markers above species respectively were effectively amplified in mungbean, indicating a certain degree of homology between these five genomes, and 97 pairs of polymorphic SSR screened from closely related species can be effectively used in the molecular genetic study of mungbean. Then the new map was compared with the map published for Azuki bean (Vigna angularis,2n=2x=22, n=11) using 32 SSR markers. Most of markers’ order in the two linkage maps was found to be highly conserved. However, chromosome rearrangement was occurred in two genomes after they diverged. In the new map, the major bruchid resistant gene was still mapped on LG I(9) with distances of less than 8.0 cM to the flanking markers, among which, the distance to SSR marker C220 which came from the LG 9 of common bean was less than 3.0 cM. Compared with the previous map, the mew location of the resistance gene is more closely linked with adjacent markers.

Key words: Mungbean, SSR, Genetic linkage map, Comparative mapping, Bruchid resistant gene

DIAO Dan, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, MA Yan-Ling. Integration of Mungbean(Vigna radiata) Genetic Linkage Map[J].Acta Agron Sin, 2010, 36(06): 932-939.

References

[1]Lin R-F(林汝法), Chai Y(柴岩), Liao Q(廖琴), Sun S-X(孙世贤). Minor Crops in China(中国小杂粮). Beijing: Chinese Agriculture Press, 2002. pp 192-209 (in Chinese)
[2]Zheng Z-J(郑卓杰). Food Legumes in China (中国食用豆类学). Beijing: Chinese Agriculture Press, 1995. pp 141-166 (in Chinese)
[3]Menancio-Hautea D, Kumar L, Danesh D, Young N D. A Genome Map for Mungbean
[4]
[Vigna radiata(L.) Wilczek] Based on DNA Genetic Markers (2n = 2x = 22) In: O'Brien J S ed. Genetic Maps 1992, A Compilation of Linkage and Restriction Maps of Genetically Studied Organisms. NY, USA: Cold Spring Harbor Laboratory Press, 1993. pp 6259-6261
[5]Lambrides C J, Lawn R J, Godwin I D. Two genetic linkage maps of mungbean using RFLP and RAPD markers
[J].Austr J Agric Resourc
[6]Humphry M E, Konduri V, Lambrides C J, Magner T, McIntyre C L, Aitken E A B, Liu C J. Development of a mungbean (Vigna radiate L.) Wilczek] RFLP linkage map and its comparison with lablab (Lablab purpureus) reveal a high level of colinearity between the two genomes. Theor Appl Genet, 2002, 105: 160-166
[7]Sholihin, Hautea D M. Molecular mapping of drought resistance in mungbean
[8]
[Vigna radiata(L.) Wilczek]: 1. Linkage map in mungbean using AFLP markers. J Biotechnol Pertanian, 2002, 7: 17-24
[9]Chen H M, Liu C A, Kuo C G, Chien C M, Sun H C, Huang C C, Lin Y C , Ku H M. Development of a molecular marker for a bruchid (Callosobruchus chinensis L.) resistance gene in mungbean. Euphytica, 2007, 157: 113-22
[10]Klos K L E, Paz M M, Marek L F, Cregan P B, Shoemaker R C. Molecular markers useful for detecting resistance to brown stem rot in soybean
[J].Crop Sci
[11]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata
[J].Mol Ecol Notes
[12]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation of microsatellite markers in mungbean, Vigna radiata
[J].Mol Ecol Notes
[13]Miyagi M, Humphry M, Ma Z Y, Lambrides C J, Bateson M, Liu C J. Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean
[J].Theor Appl Genet
[14]Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A, Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean (Vigna radiata L)
[J].Mol Ecol Notes
[15]Liu C-Y(刘长友), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Sun L(孙蕾), Mei L(梅丽), Xu N(徐宁). The screening of SSR and STS markers for genetic diversity analysis of mungbean. J Plant Genet Res (植物遗传资源学报), 2007, 8(3): 298-302 (in Chinese with English abstract)
[16]Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the Genus and other legume genera: Implications for the transferability of SSRs in plants. Mol Biol Evol, 1998, 15: 1275-1287
[17]Sharma R K, Gupta P, Sharma V, Sood A, Mohapatra T, Ahuja P S. Evaluation of rice and sugarcane SSR markers for phylogenetic and genetic diversity analyses in bamboo. Genome, 2008, 51: 91-103
[18]J Kingston R W. Berken-a new mungbean variety. Qld Agric J, 1975, 101: 659-661
[19]Lawn R J, Cottrel1. A Wild mungbean and its relatives in Australia. Biologist, 1988, 35: 267-273
[20]Rebetzke G J. Attributes of Potential Adaptive and Agronomic Significance in the Wild Mungbean (Vigna radiate L.) Wikzek ssp. Sublobata (Roxb.) Verdc.). Brisbane, Australia: MS Dissertation of University of Queensland, 1994
[21]Ji W(季伟), Wang L-X(王立新), Sun H(孙辉), Wang M-Y(王茅雁), Zhao C-P(赵昌平). Predigestion of wheat SSR analysis protocol. J Agric Biotechnol (农业生物技术学报), 2007, 15(5): 907-908 (in Chinese with English abstract)
[22]Mei L(梅丽), Wang S-H(王素华), Wang L-X(王丽侠), Liu C-Y(刘长友), Sun L(孙蕾), Xu N(徐宁), Liu C-J(刘春吉), Cheng X-Z(程须珍).Mapping of genes resistant to bruchid in mungbean using recombinant inbred lines population. Acta Agron Sin(作物学报), 2007, 33(10): 1601-1605(in Chinese with English abstract)
[23]Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for azuki bean
[24]em>Vigna angularis (Willd.) Ohwi & Ohashi]
[J].Theor Appl Genet
[25]Song X-L(宋宪亮), Sun X-Z(孙学振), Zhang T-Z(张天真). Segregation distortion and its effect on genetic mapping in plants. J Agric Biotechnol (农业生物技术学报), 2006, 14(2): 286-292 (in Chinese with English abstract)
[26]Li W-H(李卫华), Liu W(刘伟), You M-S(尤明山), Xu J(许杰), Liu C-L(刘春雷), Li B-Y(李保云), Liu G-T(刘广田). Construction of wheat molecular linkage map using different SSR markers and the polymorphism of the markers. J Triticeae Crops (麦类作物学报), 2007, 27(1): 1-6 (in Chinese with English abstract)
[27]Wang Z-L(王竹林), Liu S-D(刘曙东), Liu H-Y(刘惠远), He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民). Genetic linkage map in ‘Bainong 64 × ‘Jingshuang 16 of wheat. Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(5): 886-892 (in Chinese with English abstract)
[28]Fatokun C A, Menancio-Hautea D I, Danesh D, Young N D. Evidence for orthologous seed weight genes in cowpea and mungbean based on RFLP mapping. Genetics, 1992, 132: 841-846
[29]Pereira M G, Lee M. Identification of genomic regions affecting plant height in sorghum and maize. Theor Appl Genet, 1995, 90: 380-388
[30]Menancio-Hautea D, Fatokun C A, Kumar L, Danesh D, Young N D. Comparative genome analysis of mungbean (Vigna radiata L. Wilczek) and cowpea(V. unguiculata L.) Walpers using RFLP mapping data. Theor Appl Genet, 1993, 86: 797-810
[31]Boutin S R, Young N D, Olsen T C, Yu Z H, Shoemaker R C, Vallejos C E. Genome conservation among three legume genera detected with DNA markers. Genome, 1995, 38: 928-937
[32]Pereira M G, Lee M. Identification of genomic regions affecting plant height in shorghum and maize. Theor Appl Genet, 1995, 90: 380-388
[33]Lagercrantz U. Comparative mapping in Arabidopsis and Brassica, fine scale genome collinearity and congruence of genes controlling flowering time
[J].Plant J

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Integration of Mungbean(Vigna radiata) Genetic Linkage Map

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