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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1075-1083.doi: 10.3724/SP.J.1006.2010.01075


Identification and Expression Analysis of ZmERD16,a Ubiquitin Extension Protein Gene in Maize (Zea mays L.)

LU Yue-Shang1,2,LIU Ying-Hui2,3,**, ZHANG Deng-Feng2, SHI Yun-Su2, SONG Yan-Chun2, WANG Tian-Yu2,*, YANG De-Guang1,*, LI Yu2   

  1. 1College of Agronomy,Northeast Agricultural University,Harbin 150030,China;2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Hebei North University,Zhangjiakou 075000,China
  • Received:2010-01-05 Revised:2010-03-04 Online:2010-07-12 Published:2010-04-28
  • Contact: WANG Tian-Yu,E-mail: wangtianyu@263.net;YANG De-Guang,E-mail: ydgl@tom.com

Abstract: Ubiquitin extension protein is a fusion prot0ein of an ubiquitin monomer followed by a ribosomal protein with 52–53 animo acids or 76–78 amino acids. Ubiquitin extension proteins have been paid attention that they play important roles in responding to stresses and regulating certain developmental processes in plants. We describe here the isolation, sequence characteristics and expression analysis of ZmERD16 gene encoding a homologue of ubiquitin extension protein. ZmERD16 includes a 390 bp open reading frame (ORF) encoding an ubiquitin monomer followed by 53 animo acids, with a predicted molecular mass of 14.7582 kD and pI of 9.94. The genomic DNA and the promoter region of ZmERD16 were obtained by PCR method. The genomic DNA was composed of four exons and three introns. Promoter had some motifs that were related to light, stress, defense, development, auxin and other stresses. The tissue-specific expression analysis suggested that ZmERD16 was constitutively expressed in maize different tissues. Quantitative real-time RT-PCR results showed ZmERD16 was a multiple stresses inducible gene, induced by various stresses, such as salt, dehydration, cold, heat, PEG, methy jasmonat (MJ) and salicylic acid (SA), but not by ABA and 2,4-D. These results suggested that ZmERD16 might play an important role in various signal transduction pathways of stresses in plant.

Key words: Maize, Ubiquitin extension protein, Clone, Stress, ZmERD16

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