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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 772-777.doi: 10.3724/SP.J.1006.2011.00772

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genome-wide Analysis of MuDR-related Transposable Elements Insertion Population in Maize

FENG Jing1,FU Xue-Qian1,WANG Ting-Ting2,TAO Yong-Sheng2,GAO You-Jun1,*,ZHENG Yong-Lian1   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;
    2 Hebei Research Station of National Key Laboratory of Crop Genetic Improvement, Agricultural University of Hebei, Baoding 071000, China
  • Received:2010-10-23 Revised:2011-03-08 Online:2011-05-12 Published:2011-03-24
  • Supported by:

    This work was supported by the grants of “863” High-tech Program (No. 2006AA10A106), the China National Fundamental Fund of Personnel Training (No. J0730649) and partly supported by the open funds of the National Key Laboratory of Crop Genetic Improvement.

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Abstract: Insertional mutagenesis has now been widely used to knockout genes for functional genomics. The maize Mutator transposons hold an advantage of high activity to construct large mutant libraries. In this study, a MuDR line was used to cross with an elite Chinese maize inbred line Z31. A total of 1000 M1 individuals were planted and self-pollinated to generatetheir M2 families. Experiments were conducted to investigate the insertion specificity of MuDR related transposable elements. Six hundred and ninety-five MuDR inserted flanking sequences were isolated with a modified MuTAIL-PCR method and analyzedwith bioinformatics. Three hundred and seventy-four non-redundant insertion sites were identified and 298 of them were mapped to a single locus on the integrated maize map. The results revealed some prominent features of the MuDR-related insertions of maize: random distribution across the 10 chromosomes, preferential insertion into genic sequence and favoring some classes of functional genes.

Key words: Zea mays, Mutator (Mu) transposons, MuDR elements, Flanking sequence, Insertion sites, MuTAIL-PCR

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