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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 918-923.doi: 10.3724/SP.J.1006.2011.00918

• RESEARCH NOTES • Previous Articles     Next Articles

Expression Analysis of Three Genes from SSH Library Constructed Using Tillering Nodes of Dongnongdongmai 1 under Low Temperature

MOU Yong-Chao, CUI Hong, YU Jing, ZENG Yan, MENG Jian-Nan,CANG Jing*   

  1. College of Life Science, Northeast Agricultural University, Harbin 150030, China
  • Received:2010-10-28 Revised:2011-03-08 Online:2011-05-12 Published:2011-03-24

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Abstract: To reveal the molecular mechanism of cold resistance of winter wheat variety Dongnongdongmai 1, under natural conditions in the north region of China, we collected tillering nodes before (5°C) and in winter season (-25°C) respectively, then constructed cDNA library of Dongnongdongmai 1 containing low temperature stress related genes by suppression subtractive hybridization. A total of 300 positive clones selected from cDNA library randomly were sequenced to obtain 230 high quality expressed sequence tags (EST). After BLAST and functional annotation, we found complex and diverse types of genes in the library, including stress response genes (such as heat shock protein, CS66, Wcor8 and other related genes) and genes encoding 40S and 60S ribosomal subunit. These genes with high frequency may play an important role in protecting Dongnongdongmai 1 from injuring under low temperature. Two unknown genes and Clp selected from the cDNA library were analyzed through quantitative PCR. The expression levels of these genes in Dongnongdongmai 1 were increased in various degrees with the decrease of temperature; however, their expression patterns were different in Jimai 22 (a winter wheat cultivar with low resistance to low temperature). This result suggests that these genes may play an important role in resistance to low temperature stress.

Key words: Dongnongdongmai 1, Gene for cold resistance, Suppression subtractive hybridization, Expressed sequence tag, Real-time PCR

[1]Yu J(于晶), Zhang L(张林), Cui H(崔红), Zhang Y-X(张永侠), Cang J(苍晶), Hao Z-B(郝再彬), Li Z-F(李卓夫). Physiological and biochemical characteristics of Dongnongdongmai 1 before wintering in high-cold area. Acta Agron Sin (作物学报), 2008, 34(11): 2019–2025 (in Chinese with English abstract)
[2]Li H-X(李红霞), Zhang L-Y(张龙雨), Zhang G-S(张改生), Niu N(牛娜), Zhu Z-W(朱展望). Construction on cDNA Library from Fertility-Related Genes of Male Sterile Wheat with Aegilops kotschyi Cytoplasm by SSH. Acta Agron Sin (作物学报), 2008, 34(6): 965–971 (in Chinese with English abstract)
[3]Yao Y Y, Ni Z F, Zhang Y H, Chen Y, Ding Y H, Han Z F, Liu Z Y, Sun Q X. Identi?cation of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction. Plant Mol Biol, 2005, 58: 367–384
[4]Monroy A F, Dryanova A, Malette B, Oren D H, Farajalla M R, Liu W C, Danyluk J, Ubayasena L W C, Kane K, Scoles G J, Sarhan F, Gulick P J. Regulatory gene candidates and gene expression analysis of cold acclimation in winter and spring wheat. Plant Mol Biol, 2007, 64: 409–423
[5]Houde M, Belcaid M, Ouellet F, Danyluk J, Monroy A F, Dryanova A, Gulick P, Bergeron A, Laroche A, Links M G, MacCarthy L, Crosby W L, Sarhan F. Wheat EST resources for functional genomics of abiotic stress. BMC Genomics, 2006, 7: 149–171
[6]Chen S-Y(陈少裕). The harm of menbrances lipid peroxidation change to the plant cell. Plant Physiol Commun (植物生理学通讯), 1991, 27(2): 84–90 (in Chinese)
[7]Zhang M-S(张明生), Qi J-L(戚金亮), Du J-C(杜建厂), Yang C-X(杨春贤), Tan F(谈锋). Relationship between relative penetration of Plasmalemma and water condition under water stress and drought resistance in sweet potato. J South China Agric Univ (华南农业大学学报), 2006, 27(1): 69–75 (in Chinese with English abstract)
[8]Huang Y-B(黄毅斌), Ying C-Y(应朝阳), Ke B-N(柯碧南), Zhuang Z-G(庄重光). Study on membrane permeability of two perennial Arachis under low temperature stress. J Sichuan Grassland(四川草原), 2005, (10): 4–5
[9]Li F-S(李富生), He L-L(何丽莲). Plant’s response to non-biological stress and advances in the research of sugarcane resistance to drought and low temperature. Sugarcane (甘蔗), 2004, 11(1): 31–37 (in Chinese with English abstract)
[10]Roberts M R, Salinas J, Collinge D B. 14-3-3 proteins and the response to abiotic and biotic stress. Plant Mol Biol, 2002, 50: 1031–1039
[11]Santner A, Calderon-Villalobos L I A, Estelle M. Plant hormones are versatile chemical regulators of plant growth. Nature Chem Biol, 2009, 5: 301–307
[12]Yu J(于晶), Zhang L(张林), Cang J(苍晶), Wang X(王兴), Zhou Z-S(周子珊), Hao Z-B(郝再彬), Li Z-F(李卓夫). Effects of exogenous ABA on cold resistance and tender seedlings growth of winter wheat Dongnongdongmai 1 in cold area. J Triticeae Crops (麦类作物学报), 2008, 28(5): 883–887 (in Chinese with English abstract)
[13]Wang X(王兴), Yu J(于晶), Yang Y(杨阳), Cang J(苍晶), Li Z-F(李卓夫). Changes of endogenous hormones of winter wheat varieties with different cold-resistances under low temperature. J Triticeae Crops (麦类作物学报), 2009, 29(5): 827–831 (in Chinese with English abstract)
[14]Jaglo-Ottosen K R, Gilmour S J, Zarka D G, Schabenberger O, Thomashow M F. Arabidopsis CBF1 overexpression induces Cor genes and enhances freezing tolerance. Science, 1998, 280: 104–106
[15]Danyluk J, Perron A, Houde M, Limin A, Fowler B, Benhamou N, Sarhan F. Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat. Plant Cell, 1998, 10: 623–638
[16]Miernyk J A. Protein folding in the plant cell. Plant Physiol, 1999, 121: 695–703
[17]Mokranjac D, Sichting M, Neupert W, Hell K. Tim14, a novel key component of the import motor of the TIM23 protein translocase of mitochondria. EMBO J, 2003, 22: 4945–4956
[18]Li G-L(李国良), Li B(李冰), Liu H-T(刘宏涛), Zhou R-G(周人纲). The responses of AtJ2 and AtJ3 gene expression to environmental stresses in Arabidopsis. Acta Photophysiol Sin (植物生理与分子生物学学报), 2005, 31(1): 47–52 (in Chinese with English abstract)
[19]Keeler S J, Boettger C M, Haynes J G, Kuches K A, Johnson M M, Thureen D L, Keeler C L Jr, Kitto S L. Acquired thermotolerance and expression of the HSP100/ClpB genes of Lima bean. Plant Physiol, 2000, 123: 1121–1132
[20]Queitscha C, Hongb S W, Vierlingb E, Lindquista S. Heat stress protein 101 plays a crucial role in thermotolerance in Arabidopsis. Plant Cell, 2000, 12: 479–492
[21]Adam Z, Clarke A K. Cutting edge of chloroplast proteolysis. Trends Plant Sci, 2002, 7: 451–456
[22]Agarwal M, Sahi C, Katiyar-Agarwal S, Agarwal S, Young T, Gallie D R, Sharma V M, Ganesan K, Grover A. Molecular characterization of rice HSP101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. Plant Mol Biol, 2003, 51: 543–553
[23]Solomon M, Belenghi B, Delledonne M, Menachem E, Levine A. The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants. Plant Cell, 1999, 11: 431–443
[24]Callis J, Regulation of protein-degradation. Plant Cell, 1995, 7: 845–857
[25]Abe K, Emori Y, Kondo H, Suzuki K, Arai S. Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin): homology with animal cystatins and transient expression in the ripening process of rice seeds. J Biol Chem, 1987, 262: 16793–16797
[26]Pernas M, Sánchez-Monge R, Salcedo G. Biotic and abiotic stress can induce cystatin expression in chestnut. FEBS Lett, 2000, 467: 206–210
[27]Christova P K, Christov N K, Imai R. A cold inducible multidomain cystatin from winter wheat inhibits growth of the snow mold fungus Microdochium nivale. Planta, 2006, 223: 1207–1218
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