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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 924-929.doi: 10.3724/SP.J.1006.2011.00924

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Allelic Variations of Phytoene Synthase Genes Controlling Yellow Pigment Content in Durum Wheat (Triticum turgidum L.)

GUO Hui-Juan, CHEN Feng*, DONG Zhong-Dong,CUI Dang-Qun*   

  1. College of Agronomy / Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2010-10-28 Revised:2011-03-08 Online:2011-05-12 Published:2011-03-24

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Abstract: A total of 177 durum wheat (Triticum turgidum L.) cultivars from five countries or regions were used to identify allelic variations of phytoene synthase (PSY) genes located on chromosomes 7A and 7B using allele-specific markers of YP7A-2, YP7B-1, YP7B-2, YP7B-3, and YP7B-4. Two alleles, previously designated as Psy-A1d and Psy-A1e, were identified on chromosome 7A (Psy-A1). The distribution percentages of the two alleles were 76.8% and 23.2%, respectively. Furthermore, six allelic variants, previously designated as Psy-B1b, Psy-B1c, Psy-B1d, Psy-B1e, Psy-B1f, and Psy-B1g, were identified on chromosome 7B (Psy-B1) with distribution percentages of 9.0%, 0.6%, 0.6%, 6.9%, 25.4%, and 57.6%, respectively. Interestingly, three allelic variants of Psy-B1b, Psy-B1c, and Psy-B1d were never reported in durum wheat before. This result enriched the polymorphism of Psy1 gene in durum wheat. On Psy7A and Psy7B loci, 34, 12, and 1 cultivars were found to possess allelic combinations of Psy-A1d/Psy-B1f, Psy-A1d/Psy-B1e, and Psy-A1d/Psy-B1c, respectively, which were genotypes of high yellow pigment content. Other 28 and 1 cultivar, with Psy7A and Psy7B combinations of Psy-A1e/Psy-B1g and Psy-A1e/Psy-B1b, respectively, were associated with low content of yellow pigment. These results may help material selection in durum wheat breeding aiming at quality improvement.

Key words: Durum wheat, Phytoene synthase (Psy), Yellow pigment, Functional marker

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