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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 11-18.doi: 10.3724/SP.J.1006.2016.00011


Sequence Polymorphism and Cumulative Effect with 6-SFT-A2 of Fructan Biosynthesis Gene 6-SFT-D in Wheat

YUE Ai-Qin1, 2,LI Ang2,MAO Xin-Guo2,CHANG Xiao-Ping2,LIU Yu-Ping2,LI Run-Zhi1,JING Rui-Lian2,*   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China; 2Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing 100081, China
  • Received:2015-09-06 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Contact: 景蕊莲, E-mail: jingruilian@caas.cn, Tel: 010-82105829 E-mail:yueaiqinnd@126.com
  • Supported by:

    This research was supported by the National High Technology Research and Development Program of China (863 Program) (2011AA100501) and the National Natural Science Foundation of China (31461143024).


Gene 6-SFT encodes a key enzyme in fructan biosynthesis pathway in common wheat (Triticum aestivum L.). In this study, we analyzed the single nucleotide polymorphism (SNP) on 6-SFT-D locus in a diversity population of 23 hexaploid wheat (AABBDD) accessions and five wheat relative species (DD) by means of direct sequencing. Functional markers were developed according to the sequence polymorphism. The correlation between 6-SFT-D haplotypes and phenotypic traits and the cumulative effect of 6-SFT alleles were analyzed using a natural population consisting of 154 historical wheat accessions. Four SNPs were detected on 6-SFT-D locus in the diversity population, forming three haplotypes. However, only two 6-SFT-D haplotypes were identified in the natural population. We developed a pair of allele-specific PCR markers based on a polymorphism (T/C) at the 2850 bp site. The results of haplotype–trait association analysis showed that 6-SFT-D was significantly associated with thousand-grain weight (TGW) and spike length under well-watered conditions. HapI was superior in improving TGW. Under drought stress and well-watered conditions, wheat materials carrying both 6-SFT-D and 6-SFT-A2 had significantly higher TGW than other genotypes, suggesting that 6-SFT-D and 6-SFT-A2 have cumulative effect on TGW improvement.

Key words: Wheat, 6-SFT-D, Functional marker, SNP, Association analysis, Cumulative effect

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