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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 11-18.doi: 10.3724/SP.J.1006.2016.00011

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Sequence Polymorphism and Cumulative Effect with 6-SFT-A2 of Fructan Biosynthesis Gene 6-SFT-D in Wheat

YUE Ai-Qin1, 2,LI Ang2,MAO Xin-Guo2,CHANG Xiao-Ping2,LIU Yu-Ping2,LI Run-Zhi1,JING Rui-Lian2,*   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China; 2Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing 100081, China
  • Received:2015-09-06 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Contact: 景蕊莲, E-mail: jingruilian@caas.cn, Tel: 010-82105829 E-mail:yueaiqinnd@126.com
  • Supported by:

    This research was supported by the National High Technology Research and Development Program of China (863 Program) (2011AA100501) and the National Natural Science Foundation of China (31461143024).

Abstract:

Gene 6-SFT encodes a key enzyme in fructan biosynthesis pathway in common wheat (Triticum aestivum L.). In this study, we analyzed the single nucleotide polymorphism (SNP) on 6-SFT-D locus in a diversity population of 23 hexaploid wheat (AABBDD) accessions and five wheat relative species (DD) by means of direct sequencing. Functional markers were developed according to the sequence polymorphism. The correlation between 6-SFT-D haplotypes and phenotypic traits and the cumulative effect of 6-SFT alleles were analyzed using a natural population consisting of 154 historical wheat accessions. Four SNPs were detected on 6-SFT-D locus in the diversity population, forming three haplotypes. However, only two 6-SFT-D haplotypes were identified in the natural population. We developed a pair of allele-specific PCR markers based on a polymorphism (T/C) at the 2850 bp site. The results of haplotype–trait association analysis showed that 6-SFT-D was significantly associated with thousand-grain weight (TGW) and spike length under well-watered conditions. HapI was superior in improving TGW. Under drought stress and well-watered conditions, wheat materials carrying both 6-SFT-D and 6-SFT-A2 had significantly higher TGW than other genotypes, suggesting that 6-SFT-D and 6-SFT-A2 have cumulative effect on TGW improvement.

Key words: Wheat, 6-SFT-D, Functional marker, SNP, Association analysis, Cumulative effect

[1]Ruuska S, Rebetzke G, van Herwaarden A, Richards R, Fettell N, Tabe L, Jenkins C. Genotypic variation in water-soluble carbohydrate accumulation in wheat. Funct Plant Biol, 2006, 33: 799–809

[2]Foulkes M J, Scott R K, Sylvester-Bradley R. The ability of wheat cultivars to withstand drought in UK conditions: formation of grain yield. J Agric Sci, 2002, 138: 153–169

[3]Asseng S, van Herwaarden A F. Analysis of the benefits to wheat yield from assimilates stored prior to grain filling in a range of environments. Plant Soil, 2003, 256: 217–229

[4]Xue G P, McIntyre C L, Jenkins C L D, Glassop D, van Herwaarden A F, Shorter R. Molecular dissection of variation in carbohydrate metabolism related to water-soluble carbohydrate accumulation in stems of wheat. Plant Physiol, 2008, 146: 441–454

[5]Xue G P, McIntyre C L, Rattey A R, van Herwaarden A F, Shorter R. Use of dry matter content as a rapid and low-cost estimate for ranking genotypic differences in water soluble carbohydrate concentrations in the stem and leaf sheath of Triticum aestivum. Crop Pasture Sci, 2009, 60: 51–59

[6]McIntyre C L, Casu R E, Rattey A, Dreccer M F, Kam J W, van Herwaarden A F, Shorter R, Xue G P. Linked gene networks involved in nitrogen and carbon metabolism and levels of water soluble carbohydrate accumulation in wheat stems. Funct Integr Genomics, 2011, 11: 585–597

[7]McIntyre C L, Seung D, Casu R E, Rebetzke G J, Shorter R, Xue G P. Genotypic variation in the accumulation of water soluble carbohydrates in wheat. Funct Plant Biol, 2012, 39: 560–568

[8]Wardlaw I F, Willenbrink J. Mobilization of fructan reserves and changes in enzyme activities in wheat stems correlate with water stress during kernel filling. New Phytol, 2000, 148: 413–422

[9]Foulkes M J, Sylvester-Bradley R, Weightman R, Snape J W. Identifying physiological traits associated with improved drought resistance in winter wheat. Field Crops Res, 2007, 103: 11–24

[10]Volaire F, Lelièvre F. Production, persistence, and water soluble carbohydrate accumulation in 21 contrasting populations of Dactylis glomerata L. subjected to severe drought in the South of France. Aust J Agric Res, 1997, 48: 933–944

[11]Portes M T, Figueiredo-Ribeiro R C L, Carvalho M A M. Low temperature and defoliation affect fructan-metabolizing enzymes in different regions of the rhizophores of Vernonia herbacea. J Plant Physiol, 2008, 165: 1572–1581

[12]Zondervan K T, Cardon L R. The complex interplay among factors that influence allelic association. Nat Rev Genet, 2004, 5: 89–100

[13]Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: present status and future prospects. Plant Mol Biol, 2005, 57: 461–485

[14]Guo Z A, Song Y X, Zhou R H, Ren Z L, Jia J Z. Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene. New Phytol, 2009, 185: 841–851

[15]Su Z Q, Hao C Y, Wang L F, Dong Y N. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.) . Theor Appl Genet, 2011, 122: 211–223

[16]Jiang Q Y, Hou J, Hao C Y, Wang L F, Ge H M, Dong Y S, Zhang X Y. The wheat (T. aestivum) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits. Funct Integr Genomics, 2011, 11: 49–61

[17]Chang J Z, Zhang J N, Mao X G, Li A, Jia J Z, Jing R L. 2013. Polymorphism of TaSAP1-A1 and its association with agronomic traits in wheat. Planta, 237: 1495–1508

[18]王倩, 毛新国, 昌小平, 贾继增, 刘惠民, 景蕊莲. 小麦TaSnRK2.10的多态性及与农艺性状的关联. 中国农业科学, 2014, 44: 1865-1877

Wang Q, Mao X G, Chang X P, Jia J Z, Liu H M, Jing R L. Polymorphism of TaSnRK2.10 and its association with yield-related traits in wheat. Sci Agric Sin, 2014, 47: 1865–1877 (in Chinese with English abstract)

[19]岳爱琴, 李昂, 毛新国, 昌小平, 李润植, 贾继增, 景蕊莲. 小麦果聚糖合成酶基因6-SFT-A单核苷酸多态性分析及其定位. 中国农业科学, 2011, 44: 2216–2224

Yue A Q, Li A, Mao X G, Chang X P, Li R Z, Jia J Z, Jing R L. Single nucleotide polymorphism and mapping of 6-SFT-A gene responsible for fructan biosynthesis in common wheat. Sci Agric Sin, 2011, 44: 2216–2224 (in Chinese with English abstract)

[20]Yue A Q, LI A, Mao X G, Chang X P, Li R Z, Jing R L. Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat. Mol Breed, 2015, 35: 63 (DOI: 10.1007/s11032-015-0266-9)

[21]Zhang J N, Hao C Y, Ren Q, Chang X P, Liu G R, Jing R L. Association mapping of dynamic developmental plant height in common wheat. Planta, 2011, 234: 891–902

[22]Bradbury P J, Zhang Z W, Kroon D E, Casstevens T M, Ramdoss Y, Buckler E S. TASSEL: Software for association mapping of complex traits in diverse samples. Bioinformatics, 2007, 2: 2633–2635

[23]贾继增, 张正斌, Devos K, Gale M D. 小麦21条染色体RFLP作图位点遗传多样性分析. 中国科学C辑, 2001, 31: 13–21

Jia J Z, Zhang Z B, Devos K, Gale M D. Diversity of wheat’s twenty-one chromosomes based on RFLP analysis. Sci China Ser C, 2001, 31: 13–21 (in Chinese with English abstract)

[24]Foulkes M J, Snape J W, Shearman V J, Reynolds M P, Gaju O, Sylvester-Bradley R. Genetic progress in yield potential in wheat: recent advances and future prospects. J Agric Sci, 2007, 145: 17–29

[25]Zhang B, Li W Y, Chang X P, Li R Z, Jing R L. Effects of favorable alleles for water-soluble carbohydrates at grain filling on grain weight under drought and heat stresses in wheat. PLoS One, 2014, 9: e102917

[26]Li W Y, Zhang B, Li R Z, Chang X P, Jing R L. Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat. PLoS One, 2015, 10: e0119438

[27]Yang D L, Jing R L, Chang X P, Li W. Identification of quantitative trait loci and environmental interactions for accumulation and remobilization of water-soluble carbohydrates in wheat (Triticum aestivum L.) stems. Genetics, 2007, 176: 571–584

[28]Cordell H J. Detecting gene-gene interactions that underlie human diseases. Nat Rev Genet, 2009, 10: 392–404

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