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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 1-10.doi: 10.3724/SP.J.1006.2016.00001

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Regulating Effects of GmMYB042 Gene on Flavonoid Biosynthesis

DU Hai1, RAN Feng1,MA Shan-Shan1,KE Yun-Zhuo1,SUN Li-Ping1,LI Jia-Na1,*,TANG Yi-Xiong2,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-06-08 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Supported by:

    This research was supported by National Natural Science Foundation of China (31471528), Postdoctoral Science Foundation of China (2014M552297) and Fundamental Research Funds for the Central Universities of China (SWU113104, XDJK2014B035).

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Abstract:

MYB transcription factor is one of the largest transcription factor gene families in land plants, and is involved in a myriad of regulatory processes, such as secondary metabolism. In the present study, the expression profiles and function of GmMYB042 gene were systematically studied. In order to investigate the roles of the conserved amino acid motif PDLNLELTIS and a predicted zinc finger region at its C-terminal, a series of sequence deletions of these two regions were made by PCR method. Subsequently, the corresponding over-expression constructs of GmMYB042 gene and its mutants were made and transformed into tobacco NC89 with Agrobacterium LBA4404, respectively. Expression analyses revealed that GmMYB042 gene was expressed in nodule, root, stem, leaf, flower, pod and seed of soybean, and with a relative higher expression level in stem, flower and seed; its expression could be induced by PEG, high salt, low temperature and UV-B radiation stresses. Over-expression analyses showed that the expressions of some enzyme genes in flavonoid biosynthesis pathway (including PAL, CHS, CHI, and FLS) were obviously increased in GmMYB042 transgenic lines, resulting in an increased content of the flavonoid compounds. Accordingly, the transcription levels of the corresponding enzyme genes involved in flavonoid biosynthesis pathway were decreased in the transgenic lines of GmMYB042 mutants, further supporting the conclusion of regulating role of GmMYB042 gene in tobacco flavonoid biosynthesis pathway.

Key words: MYB transcription factor, Flavonoid, Deletion mutation, Functional research

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