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Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (12): 2950-2961.doi: 10.3724/SP.J.1006.2024.44057

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Metabolome and transcriptome analysis of flavonoids in peanut testa

JIN Xin-Xin(), SU Qiao, SONG Ya-Hui, YANG Yong-Qing, LI Yu-Rong, WANG Jin()   

  1. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Hebei Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, Hebei, China
  • Received:2024-04-08 Accepted:2024-08-15 Online:2024-12-12 Published:2024-08-27
  • Contact: *E-mail: wangjinnky@163.com
  • Supported by:
    China Agriculture Research System of MOF and MARA(CARS-13);Hebei Agriculture Research System(HBCT2024040101);Hebei Agriculture Research System(HBCT2024040204);Science and Technology Innovation Team of Modern Peanut Seed Industry(21326316D);Talents Construction Project of Science and Technology Innovation of Hebei Academy of Agriculture and Forestry Sciences(2022KJCXZX-LYS-11)

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Abstract:

To explore the regulatory mechanisms of flavonoid components and anthocyanin biosynthesis in the color formation of peanut testa, we conducted a study using five peanut cultivars with different testa colors: pink, red, white, black, and speckled (red and white). The key metabolites and genes related to anthocyanin biosynthesis were identified using flavonoid metabolomics and transcriptomics. Our results revealed the identification of 329 flavonoid metabolites in peanut testa, with flavonols being the most abundant in both relative content and variety. We detected 19 types of anthocyanidins, including cyanidin, delphinidin, and petunidin. Most anthocyanidins were modified with glucoside, morbuside, rutin, galactoside, and other glycosides. Notably, the anthocyanin content in black testa was 22.60-66.72 times higher than that in other testa colors, with cyanidin-3-O-sambutin being the most prevalent in black testa. Different metabolites were significantly enriched in anthocyanin biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, and isoflavone biosynthesis pathways in colored testa compared to white testa. The high expression levels of structural genes in the flavonoid and anthocyanin biosynthesis pathways promoted anthocyanin accumulation in colored testa. Anthocyanin reductase (ANR) and glycosyltransferase (UGT) emerged as candidate genes involved in testa pigmentation, with the competition and activity of UGT and ANR against substrate anthocyanin determining the color pattern of peanut testa. These findings elucidate the regulatory mechanisms of flavonoid substances in peanut testa color, providing valuable references for the breeding of special peanut varieties and the utilization of their nutritional value based on color differences.

Key words: peanut, testa, flavonoids, metabolome, transcriptome

Fig. 1

Phenotypic differences of the five color testa in peanut PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa."

Fig. 2

Classification (A) and relative contents (B) of flavonoids metabolites in peanut testa of different cultivars PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa."

Fig. 3

Relative contents of the anthocyanins in the testa of different peanut cultivars PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa. Different lowercase letters in the figure indicate significant differences between samples at the 0.05 probability level."

Fig. 4

Number of DEMs (A) and DEGs (B) in WT vs BT, WT vs CT, WT vs RT, WT vs PT comparisons PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa."

Fig. 5

KEGG enrichment pathway of DEMs (A) and DEGs (B) in WT vs BT, WT vs CT, WT vs RT, WT vs PT comparisons PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa."

Fig. 6

Content heatmap of DEMs enriched in the flavonoid and anthocyanin biosynthesis pathway (A) and correlation heatmap of the DEMs and DEGs (B) PT: pink testa; WT: white testa; RT: red testa; CT: multicolor testa; BT: black testa."

Table 1

FPKM-value of DEGs enriched by flavonoid and anthocyanin biosynthesis pathway"

酶种类
Enzyme class		 差异表达基因
DEGs		 黑皮
BT		 花斑皮
CT		 粉皮
PT		 红皮
RT		 白皮
WT
查尔酮合成酶
Chalcone synthase (CHS)
 A01g004662 0.60 0.87 0.92 0.20 0.95
A03g016137 176.29 177.86 68.54 59.53 30.44
A05g024845 25.61 16.35 1.10 3.62 0.38
B01g052224 1.05 0.25 0.57 0.59 1.14
B03g067612 188.56 213.25 96.22 78.82 46.62
B05g078749 1.75 0.45 0.24 0.14 0
B05g078750 3.59 1.19 0.38 0.31 0.03
B06g084455 163.86 37.62 101.96 109.79 72.33
Scaffold6g107977 0.19 1.03 0.45 0.13 0.19
Scaffold6g107983 0.66 1.87 1.58 0.48 1.01
novel.4300 0.48 1.10 0.66 0.24 0.35
novel.4302 0.38 1.14 1.05 0.21 0.77
查尔酮异构酶
Chalcone isomerase (CHI)
 A04g020659 1.73 1.35 2.09 1.76 0.03
A09g041790 0.32 0.47 0.60 0.38 0.22
A10g048771 46.61 27.58 23.76 15.80 12.38
B02g057981 3.31 1.62 3.35 7.69 4.10
B09g099540 21.24 13.24 10.51 9.67 4.56
B10g104123 67.03 47.14 36.85 32.85 20.07
novel.5812 0.79 0.31 0.73 2.07 0.70
黄烷酮羟化酶
Flavanone 3-hydroxylase (F3H)		 A01g003995 81.12 59.87 14.24 10.71 1.85
novel.3235 52.47 47.26 14.83 10.56 1.32
类黄酮羟化酶
Flavonoid 3'-hydroxylase (F3'H)		 A10g047831 56.48 26.57 12.05 7.12 4.27
B10g105286 146.62 49.23 34.29 15.90 3.87
二氢黄酮醇还原酶
Dihydroflavonol reductase (DFR)		 B06g086139 135.00 130.73 26.27 28.59 19.30
黄酮醇合成酶
Flavonol synthetase (FLS)		 A07g032578 5.63 27.24 13.56 16.49 12.47
B10g102931 1.79 2.23 3.64 1.65 1.69
无色花青素还原酶
Leucocyanidin reductase (LAR)		 A05g022192 28.44 15.87 0.94 0.09 0.02
B05g076540 24.65 12.28 0.82 0.09 0
花青素合成酶
Anthocyanidin synthase (ANS)		 B10g102589 0.22 0.30 0.59 0.36 0.95
Scaffold1g106620 26.42 17.92 6.72 3.46 0.18
花青素还原酶
Anthocyanin reductase (ANR)		 A03g011741 5.71 1.73 6.06 0.36 0.11
A03g016167 0.82 0.06 0.39 0.10 0.34
A04g018753 6.46 3.74 3.46 4.53 3.07
B03g067636 4.05 0.81 1.97 0.96 1.64
novel.367 22.70 0.43 4.77 0.18 0.11
糖基转移酶
UDP-glycosyltransferases (UGT)		 A03g015165 5.32 8.97 2.54 1.92 0.01
B10g101189 0 1.32 0 0.16 0.17

Fig. 7

Expression pattern of anthocyanin biosynthesis"

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