Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 1-10.doi: 10.3724/SP.J.1006.2016.00001


Regulating Effects of GmMYB042 Gene on Flavonoid Biosynthesis

DU Hai1, RAN Feng1,MA Shan-Shan1,KE Yun-Zhuo1,SUN Li-Ping1,LI Jia-Na1,*,TANG Yi-Xiong2,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-06-08 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Supported by:

    This research was supported by National Natural Science Foundation of China (31471528), Postdoctoral Science Foundation of China (2014M552297) and Fundamental Research Funds for the Central Universities of China (SWU113104, XDJK2014B035).


MYB transcription factor is one of the largest transcription factor gene families in land plants, and is involved in a myriad of regulatory processes, such as secondary metabolism. In the present study, the expression profiles and function of GmMYB042 gene were systematically studied. In order to investigate the roles of the conserved amino acid motif PDLNLELTIS and a predicted zinc finger region at its C-terminal, a series of sequence deletions of these two regions were made by PCR method. Subsequently, the corresponding over-expression constructs of GmMYB042 gene and its mutants were made and transformed into tobacco NC89 with Agrobacterium LBA4404, respectively. Expression analyses revealed that GmMYB042 gene was expressed in nodule, root, stem, leaf, flower, pod and seed of soybean, and with a relative higher expression level in stem, flower and seed; its expression could be induced by PEG, high salt, low temperature and UV-B radiation stresses. Over-expression analyses showed that the expressions of some enzyme genes in flavonoid biosynthesis pathway (including PAL, CHS, CHI, and FLS) were obviously increased in GmMYB042 transgenic lines, resulting in an increased content of the flavonoid compounds. Accordingly, the transcription levels of the corresponding enzyme genes involved in flavonoid biosynthesis pathway were decreased in the transgenic lines of GmMYB042 mutants, further supporting the conclusion of regulating role of GmMYB042 gene in tobacco flavonoid biosynthesis pathway.

Key words: MYB transcription factor, Flavonoid, Deletion mutation, Functional research

[1] Klempnauer K H, Gonda T J, Bishop J M. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene. Cell, 1982, 31: 453–463

[2] Du H, Zhang L, Liu L, Tang X F, Yang W J, Wu Y M, Huang Y B, Tang Y X. Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry (Mosc), 2009, 74: 1–11

[3] Du H, Wang Y B, Xie Y, Liang Z, Jiang S J, Zhang S S, Huang Y B, Tang Y X. Genome-wide identification and evolutionary and expression analyses of MYB-related genes in land plants. DNA Res, 20: 437–448

[4] Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends Plant Sci, 2010, 15: 573–581

[5] Weston K. Myb proteins in life, death and differentiation. Curr Opin Genet Dev, 1998, 8: 76–81

[6] Paz-Ares J, Ghosal D, Wienand U, Peterson P A, Saedler H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J, 1987, 6: 3553–3558

[7] Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol, 2001, 4: 447–456

[8] Du H, Yang S S, Liang Z, Feng B R, Liu L, Huang Y B, Tang Y X. Genome-wide analysis of the MYB transcription factor superfamily in soybean. BMC Plant Biol, 2012, 12:106

[9] Wilkins O, Nahal H, Foong J, Provart N J, Campbell M M. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol, 2009, 149: 981–993

[10] Stracke R, Jahns O, Keck M, Tohge T, Niehaus K, Fernie A R, Weisshaar B. Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation. New Phytol, 2010, 188: 985–1000

[11] Chen B J, Wang Y, Hu Y L, Wu Q, Lin Z P. Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia. Plant Sci, 2005, 168: 493–500

[12] Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant cell, 2003, 15: 63–78

[13] Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta S L, Tonelli C. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol, 2005, 15: 1196–1200

[14] Seo P J, Xiang F, Qiao M, Park J Y, Lee Y N, Kim S G, Lee Y H, Park W J, Park C M. The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiol, 2009, 151: 275–289

[15] Oh J E, Kwon Y, Kim J H, Noh H, Hong S W, Lee H. A dual role for MYB60 in stomatal regulation and root growth of Arabidopsis thaliana under drought stress. Plant Mol Biol, 2011, 77: 91–103

[16] Du H, Huang Y, Tang Y. Genetic and metabolic engineering of isoflavonoid biosynthesis. Appl Microbiol Biotechnol, 2010, 86:1293–1312

[17] Buer C S, Imin N, Djordjevic M A. Flavonoids: new roles for old molecules. J Integr Plant Biol, 2010, 52: 98–111

[18] Yang C S, Landau J M, Huang M T, Newmark H L. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr, 2001, 21: 381–406

[19] Ross J A, Kasum C M.: Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu Rev Nutr, 2002, 22: 19–34

[20] Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C. Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J, 2000, 19: 6150–6161

[21] Legay S, Sivadon P, Blervacq A S, Pavy N, Baghdady A, Tremblay L, Levasseur C, Ladouce N, Lapierre C, Séguin A, Hawkins S, Mackay J, Grima-Pettenati J. EgMYB1, an R2R3 MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar. New Phytol, 2010, 188: 774–786

[23] Sonbol F M, Fornalé S, Capellades M, Encina A, Touriño S, Torres J L, Rovira P, Ruel K, Puigdomènech P, Rigau J, Caparrós-Ruiz D. The maize ZmMYB42 represses the phenylpropanoid pathway and affects the cell wall structure, composition and degradability in Arabidopsis thaliana. Plant Mol Biol, 2009, 70: 283–296

[24] Mehrtens F, Kranz H, Bednarek P, Weisshaar B. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol, 2005, 138: 1083–1096

[25] Stracke R, Ishihara H, Huep G, Barsch A, Mehrtens F, Niehaus K, Weisshaar B. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling. Plant J, 2007, 50: 660–677

[26] Czemmel S, Stracke R, Weisshaar B, Cordon N, Harris N N, Walker A R, Robinson S P, Bogs J. The grapevine R2R3-MYB transcription factor VvMYBF1 regulates flavonol synthesis in developing grape berries. Plant Physiol, 2009, 151: 1513–1530

[27] 杨文杰, 杜海, 方芳, 杨婉身, 吴燕民, 唐益雄. 大豆两个MYB 转录因子基因的克隆及表达分析. 中国农业科学, 2008, 41: 961–970

Yang W J, Du H, Fang F, Yang W S, Wu Y M, Tang Y X. Cloning and characterization of two new MYB transcription factor genes from soybean. Sci Agric Sin, 2008, 41: 961–970 (in Chinese with English abstract)

[28] 杜海, 刘蕾, 唐晓凤, 高杰, 杨文杰, 吴燕民, 黄玉碧, 唐益雄. 大豆MYB 转录因子GmMYBZ2 的鉴定、突变分析及原核. 农业生物技术学报, 2009, 17: 301–306

Du H, Liu L, Tang X F, Gao J, Yang W J, Wu Y M, Huang Y B, Tang Y X. Characterization, expression and transcriptional activation analysis of GmMYBZ2 in soybean. J Agric Biotechnol, 2009, 17: 301–306 (in Chinese with English abstract)

[29] Severin A J, Woody J L, Bolon Y T, Joseph B, Diers B W, Farmer A D, Muehlbauer G J, Nelson R T, Grant D, Specht J E, Graham M A, Cannon S B, May G D, Vance C P, Shoemaker R C. RNA-Seq Atlas of Glycine max: a guide to the soybean transcriptome. BMC Plant Biol, 2010, 10: 160

[30] Libault M, Farmer A, Joshi T, Takahashi K, Langley R J, Franklin L D, He J, Xu D, May G, Stacey G. An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants. Plant J, 2010, 63: 86–99

[31] Lodovico T, Angel M, Adrian P, Mackay S, Francisco A, Culianez-Macia, Keith R, Cathie M. The AmMYB308 and AmMYB330 transcription factors from antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell, 1998, 10: 135–154

[32] Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C.Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J, 2000, 19: 6150–6161

[33] Du H, Liang Z, Zhao S, Nan M G, Phan Tran L S, Lu K, Huang Y B, Li J N. The evolutionary history of R2R3-MYB proteins across 50 eukaryotes: new insights into subfamily classification and expansion. Sci Rep, 2015, 5: 11037

[1] HUANG Wei, GAO Guo-Ying, WU Jin-Feng, LIU Li-Li, ZHANG Da-Wei, ZHOU Ding-Gang, CHENG Hong-Tao, ZHANG Kai-Xuan, ZHOU Mei-Liang, LI Mei, YAN Ming-Li. Regulation of flavonoid synthesis by BjA09.TT8 and BjB08.TT8 genes in Brassica juncea [J]. Acta Agronomica Sinica, 2022, 48(5): 1169-1180.
[2] HE Jun-Yu, ZHONG Wei, CHEN Yun-Qiong, WANG Wei-Bin, XIONG Jing-Lei, JIANG Ya-Li, SHI Hui-Meng, CHEN Sheng-Wei. Analysis on the accumulation characteristics of seven flavonoids at grain development stage in barley [J]. Acta Agronomica Sinica, 2021, 47(8): 1624-1630.
[3] QIU Hong-Mei, CHEN Liang, HOU Yun-Long, WANG Xin-Feng, CHEN Jian, MA Xiao-Ping, CUI Zheng-Guo, ZHANG Ling, HU Jin-Hai, WANG Yue-Qiang, QIU Li-Juan. Research progress on genetic regulatory mechanism of seed color in soybean (Glycine max) [J]. Acta Agronomica Sinica, 2021, 47(12): 2299-2313.
[4] GAO Guo-Ying, WU Xiao-Fang, HUANG Wei, ZHOU Ding-Gang, ZHANG Da-Wei, ZHOU Mei-Liang, ZHANG Kai-Xuan, YAN Ming-Li. Regulation of flavonoid pathway by BjuB.KAN4 gene in Brassica juncea [J]. Acta Agronomica Sinica, 2020, 46(9): 1322-1331.
[5] WANG Yan-Hua, JIAN Hong-Jiu, QIU Xiao, LI Jia-Na. Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L. [J]. Acta Agronomica Sinica, 2020, 46(11): 1678-1689.
[6] HUANG Qi-Xiu,QU Yan-Ying,YAO Zheng-Pei,LI Meng-Yu,CHEN Quan-Jia*. Correlation between Resistance to Fusarium Wilt and Expression of Flavonoid Metabolism Related Genes in Gossypium barbadense L. [J]. Acta Agron Sin, 2017, 43(12): 1791-1801.
[7] SHAN Tian-Lei,HONG Yan-Tao,DU Li-Pu,XU Hui-Jun,WEI Xue-Ning,ZHANG Zeng-Yan*. Development and Characterization of TaMYB86-Overexpressing Transgenic Wheat Lines with Resistance to Common Root Rot [J]. Acta Agron Sin, 2016, 42(10): 1429-1436.
[8] GAO Wei**,LIU Hui-Li**,TIAN Xin-Quan,ZHANG Hui,SONG Jie,YANG Yong,LONG Lu,SONG Chun-Peng*. Cloning, Expression, and Functional Analysis of Transcription Factor GeneGbMYB60 in Cotton [J]. Acta Agron Sin, 2016, 42(09): 1342-1351.
[9] HE Hai-Wang,PAN Hua-Qing,ZHANG Nao-Dan,HE Long-Fei*. Chromolaena odorata Flavonoid 3’-hydroxylase Gene Cloning and Its Expression in Tobacco [J]. Acta Agron Sin, 2015, 41(03): 479-486.
[10] QU Cun-Min,LU Kun,LIU Shui-Yan,BU Hai-Dong,FU Fu-You,WANG Rui,XU Xin-Fu,LI Jia-Na. SNP Detection and Analysis of Genes for Flavonoid Pathway in Yellow- and Black-Seeded Brassica napus L. [J]. Acta Agron Sin, 2014, 40(11): 1914-1924.
[11] WANG Nuo-Han,YU Ji-Wen,WU Man,MA Qi-Feng1,LI Xing-Li,PEI Wen-Feng,LI Hai-Jing,HUANG Shuang-Ling,ZHANG Jin-Fa,YU Shu-Xun. Cloning, Expression, and Functional Analysis of GhMYB0 Gene from Cotton (Gossypium hirsumtum L.) [J]. Acta Agron Sin, 2014, 40(09): 1540-1548.
[12] WU Lei, WANG Dan,SU Wen-Yue,GUO Chang-Hong,SHU Yong-Jun. Developing InDel Markers from Aegilops Genus Based on Comparative Genomics [J]. Acta Agron Sin, 2012, 38(07): 1334-1338.
[13] YI Jin-Xin, XU Zhao-Long, WANG Jun-Feng, ZHANG Da-Yong, HE Xiao-Lan, ZULFIQAR Ali, ZHU Hong-Run, MA Hong-Xiang, SANGEETA Dhaubhade. GmCHS8 and GmIFS2 Gene Co-Determine Accumulation of Isoflavonoid in Soybean [J]. Acta Agron Sin, 2011, 37(04): 571-578.
[14] ZHOU Xian-Yao, DONG Na, LIU Gong-Xia, ZHANG Fu-Yu, ZHANG Ceng-Yan. Cloning of Wheat TaPIM1 Gene and Analysis of Disease Resistance in TaPIM1 Transgenic Tobacco [J]. Acta Agron Sin, 2010, 36(06): 911-917.
[15] JIA Dong-Sheng;MAO Xin-Guo;JING Rui-Lian;ZHANG Xiao-Ke;CHANG Xiao-Ping. Cloning and Expression of Transcription Factor TaMyb2s in Wheat [J]. Acta Agron Sin, 2008, 34(08): 1323-1329.
Full text



No Suggested Reading articles found!