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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 479-486.doi: 10.3724/SP.J.1006.2015.00479

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Chromolaena odorata Flavonoid 3’-hydroxylase Gene Cloning and Its Expression in Tobacco

HE Hai-Wang,PAN Hua-Qing,ZHANG Nao-Dan,HE Long-Fei*   

  1. Agricultural College of Guangxi University, Nanning 530004, China
  • Received:2014-08-12 Revised:2014-12-19 Online:2015-03-12 Published:2014-12-29
  • Contact: 何龙飞, E-mail: lfhe@gxu.edu.cn, Tel: 0771-3235212-801 E-mail:gxhhwn@gxaas.net

Abstract:

A flavonoid 3’-hydroxylase gene cDNA sequence was cloned by RACE and RT-PCR techniques from Chromolaena odorata. The obtained full-length cDNA was named as CoF3H with GenBank accession number HQ268505.1. It is 1628 bp in length, containing a 1524 bp open reading frame, encoding 507 amino acid residues. The amino acid sequence of CoF3H contains cytochrome P450 domain and cysteineheme binding region (F××G×R×C×G). Homology analysis by DNAMAN software showed that the deduced CoF3H protein was highly homologous to F3’H proteins from different species. CoF3H gene was transferred into tobacco by agrobacterium-mediated genetic transformation. There was higher content of flavonvoids in T2 transgenic tobacco than in the wild type. This result shows that CoF3H plays an important role in flavonoid biosynthesis, and provides a basis for researches on allelopathy and comprehensive utilization of C. odorata in the future.

Key words: Chromolaena odorata, Flavonoid 3’-hydroxylase, Clone, Expression

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