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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1676-1682.doi: 10.3724/SP.J.1006.2011.01676

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Quantitative Determination of Peanut Seed Lipase Activity and Its Correlation with Storage Characteristics

AO Qi1,3,ZHANG Ying1,2,*,LIU Ze1,ZHANG Zheng-Zhu3,*,WU Yue-Jin2,YU Shan-Lin4,ZHANG Chen3,TENG Bin1,WU Jing-De1,JIANG Lan1   

  1. 1 Rice Research Institute, Anhui Academy of Agricultural Sciences / Anhui Province Key Laboratory of Rice Genetics and Breeding, Hefei 230031, China; 2 Anhui Province Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, China; 3 Tea and Herbs Key Laboratory Safety, Anhui Agricultural University, Ministry of Agriculture, Hefei 230036, China; 4 Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2011-02-12 Revised:2011-05-27 Online:2011-09-12 Published:2011-06-28
  • Contact: 张瑛, E-mail: zhangying56888@sina.com; 张正竹, E-mail: zzz@ahau.edu.cn, Tel: 0551-2160182, 0551-2160453

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Abstract: Based on colorimetric determination method reported for rice lipase, we established a new method improving and optimizing the measurement for peanut seed lipase activity, with the optimum sampling amount of 5 mg and substrate concentration of 30 mg mL–1. The method was validated to be accurate, specific and well repeatable. The lipase activities of 216 peanut germplasm resources were detected, with significant difference (P<0.001) among them. Five with higher lipase activity and five with lower lipase activity were screened, and each two of which were selected to perform an accelerated-aging experiment under high temperature and high moisture. The results indicated that, with extension of storage time, the germination rate of peanut with lower lipase activity decreased slowly, while that of peanut with higher lipase decreased rapidly, showing a negative correlation betweenseed vigor and peanut lipase activity (correlation coefficient was –0.8875). The study provides a reference for the further research in the effect of lipase on peanut storage characteristics.

Key words: Peanut, Lipase activity, Quantitative determination, Storage characteristics

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