Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 23-35.doi: 10.3724/SP.J.1006.2012.00023

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Characterizations of a Mutant Gene hw-1(t) for Green-revertible Albino, High Tillering and Dwarf in Rice (Oryza sativa L.)

GUO Tao**,HUANG Xuan**,HUANG Yong-Xiang,LIU Yong-Zhu,ZHANG Jian-Guo,CHEN Zhi-Qiang*,WANG Hui*   

  1. South China Agricultural University / National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2011-05-04 Revised:2011-09-12 Online:2012-01-12 Published:2011-11-07
  • Contact: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

PDF

1857

Cited

4

Abstract: A rice (Oryza sativa L.) mutant hfa-1 exhibiting green-revertible albino, high-tillering dwarf was detected from a M2 population of an American variety Francis by space mutagenesis. hfa-1 displayed distinctive albino before 3rd leaf stage but finally turned to normal green after 3rd leaf stage, and the expression of this phenotype was conditionally controlled by development and temperature. Examining ultrastructure and measuring chlorophyll content indicated that hfa-1 showed albino due to abnormal development of chloroplast, which resulted in inhibiting chlorophyll synthesis and decreasing photosynthetic efficiency. Moreover, the increased tiller number of hfa-1 was ascribed to initiate more higher-order tiller buds. Further endogenous hormones analysis demonstrated that the enhanced tillering capacity of hfa-1 might not result from inhibiting the synthesis of IAA. In addition, the dwarfism of hfa-1 was caused by shortening internodes and was independent of biosynthesis and signal transduction of GA. Genetic analysis indicated that the phenotype of green-revertible albino, high-tillering dwarf in hfa-1 was controlled by a recessive nucleic gene, namely hw-1(t). Using a large F2 mapping population derived from a cross between hfa-1 and an japonica rice variety, 02428, hw-1(t) was fine mapped into a 46.9 kb of physical distance between two InDel markers, HW27 and HW7 on chromosome 4, where 13 open reading frames were predicted. In the mapping interval LOC_Os04g57320 encoded a IMMUTANTS protein, which was the most properly candidate gene of hw-1(t).

Key words: Rice (Oryza sativa L.), Green-revertible albino, High-tillering dwarf, hw-1(t), Fine mapping

[1]Gustafsson A. The plastid development in various types of chlorophyll mutations. Hereditas, 1942, 28: 483-492
[2]Tanya G F, Staehelin L A. Partial blocks in the early steps of the chlorophyll synthesis pathway: a common feature of chlorophyll b-deficient mutants. Physiol Plant, 1996, 97: 311-320
[3]Wu D-X(吴殿星), Shu Q-Y(舒庆尧), Xia Y-W(夏英武), Liu G-F(刘贵付). 60Co gamma-ray induced temperature-regulatory leaf color albino mutated gene expression mutant line in rice (Oryza sativa L.). Sci Agric Sin (中国农业科学), 1997, 30(3): 95-95 (in Chinese with English abstract)
[4]Zhao Y, Wang M L, Zhang Y Z, Du L F, Pan T. A chlorophyll-reduced seedling mutant in oilseed rape, Brassica napus, for utilization in F1 hybrid production. Plant Breed, 2000, 119: 131-135
[5]Gan S, Amasino R M. Inhibition of leaf senescence by autoregulated production of cytokinin. Science, 1995, 270: 1986-1988
[6]Fambrini M, Castagna A, Vecchia F D. Characterization of a pigment-deficient mutant of sunflower (Helianthus annuus L.) with abnormal chloroplast biogenesis, reduced PS II activity and low endogenous level of abscisic acid. Plant Sci, 2004, 167: 79-89
[7]Parks B M, Quail P H. Phytochrome-deficient hy1 and hy2 long hypocotyls mutants of Arabidopsis are defective in phytochrome chromophore biosynthesis. Plant Cell, 1991, 3: 1177-1186
[8]Singh U P, Prithiviraj B, Sarma B K. Development of Erysiphe pisi (powdery mildew) on normal and albino mutants of pea (Pisum sativum L.). J Phytopathol, 2000, 148: 591-595
[9]Xing S, Miao J, Li S, Qin G, Tang S, Li H, Gu H, Qu L J. Disruption of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) gene results in albino, dwarf and defects in trichome initiation and stomata closure in Arabidopsis. Cell Res, 2010, 20: 688-700
[10]Schwartz S H, Qin X, Zeevaart J A. Elucidation of the indirect pathway of abscisic acid biosynthesis by mutants, genes and enzymes. Plant Physiol, 2003, 131: 1591-1601
[11]Agrawal G K, Yamazak I M, Kobayash I M. Screening of the rice viviparous mutants generated by endogenous retrotransposon Tos17 insertion tagging of a zeaxanthin epoxidase gene and a novel OsTATC gene. Plant Physiol, 2001, 125: 1248-1257
[12]Beale S I. Green genes gleaned. Trends Plant Sci, 2005, 10: 301-312
[13]Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. Defect in non-yellow coloring 3, an alpha/beta hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J, 2009, 59: 940-952
[14]Terry M J, Kendrick R E. Feedback inhibition of chlorophyll synthesis in the phytochrome chromophore-deficient aurea and yellow-green-2 mutants of tomato. Plant Physiol, 1999, 119: 143-152
[15]Chen G, Bi Y R, Li N. EGY1 encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development. Plant J, 2005, 41: 364-375
[16]Kushnir S, Babiychuk E, Storozhenko S, Davey M W, Papenbrock J, Rycke R D, Engler G, Stephan U W, Lange H, Kispal G, Lill R, Van M M. A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starik. Plant Cell, 2001, 13: 89-100
[17]Shu Q-Y(舒庆尧), Wu D-X(吴殿星), Xia Y-W(夏英武), Liu G-F(刘贵付). Study on greenism characteristics of greenable albino mutation line W25 of rice (Oryza sativa L.). J Zhejiang Agric Univ (浙江农业大学学报), 1996, 22(2): 219-220 (in Chinese with English abstract).
[18]Zhao H-J(赵海军), Wu D-X(吴殿型), Shu Q-Y(舒庆尧), Shen S-Q(沈圣泉), Ma C-X(马传喜). Breeding and characteristics of photo-thermo sensitive genic male sterile rice Yutu S Labeled with green-revertible albino leaf marker. Chin J Rice Sci (中国水稻科学), 2004, 18(6): 515-521 (in Chinese with an English abstract)
[19]Zhang Y(张毅), Lü J(吕俊), Li Y-F(李云峰), Yang K(杨昆), Shen F-C(沈福成), Zhang Q-L(张巧玲), Peng Q-L(彭其莲), Zhou Y-L(周亚林), He G-H(何光华). Effects of green-revertible albino gene on the agronomy traits and appearance quality in rice. Acta Agron Sin (作物学报), 2008, 34(2): 284-289 (in Chinese with an English abstract)
[20]Guo S-W(郭士伟), Wang Y-F(王永飞), Ma S-M(马三梅), Li X(李霞), Gao D-Y(高东迎). Genetic analysis and fine mapping of a green-revertible albino leaf mutant in rice. Chin J Rice Sci (中国水稻科学), 2011, 25(1): 95-98 (in Chinese with an English abstract)
[21]Shen S-Q(沈圣泉), Shu Q-Y(舒庆尧), Bao J-S(包劲松), Wu D-X(吴殿星), Cui H-R(崔海瑞), Xia Y-W(夏英武). Development of a greenable leaf colour mutant Baifeng A and its application in hybrid rice production. Chin J Rice Sci (中国水稻科学), 2004, 18(1): 34-38 (in Chinese with English abstract)
[22]Wu W(吴伟), Liu X(刘鑫), Shu X-L(舒小丽), Shu Q-Y(舒庆尧), Xia Y-W(夏英武), Wu D-X(吴殿星). Two-line hybrid rice mail sterile line ‘NHR111S’ with a marker of green-revertible albino leaves. J Nucl Agric Sci (核农学报), 2006, 20(2): 103-105 (in Chinese with English abstract)
[23]Li R-Q(李瑞清), Wu L-Q(武立权), Shu Q-Y(舒庆尧), Zhao H-J(赵海军), Wu D-X(吴殿星), Wang R-F(王荣富). Characterization of a new green-revertible mutant G9 of rice. J Nucl Agric Sci (核农学报), 2010, 24(5): 881-886 (in Chinese with English abstract)
[24]Fang X-T(房贤涛), Ma H-L(马洪丽), Zhao F-Y(赵福源), Zhang Q-Q(章清杞), Zhang S-B(张书标). Studied on the breeding application of six photo-thermo-sensitive genic male sterile line mutants with greenable albino leaf. Chin Agric Sci Bull (中国农学通报), 2011, 27(1): 45-51 (in Chinese with English abstract)
[25]Liu G-F(刘贵付), Shu Q-Y(舒庆尧), Xia Y-W(夏英武). Utilization of Greenable albino mutation lines of thermosensitive genic male sterile rice (Oryza sativa L. ssp indica). J Nucl Agric Sci (核农学报), 1996, 10(3): 129-132 (in Chinese with English abstract)
[26]Chen T, Zhang Y, Zhao L, Zhu Z, Lin J, Zhang S, Wang C. Physiological character and gene mapping in a new green- revertible albino mutant in rice. J Genet Genomics, 2007, 34: 331-338
[27]Chen T, Zhang Y, Zhao L, Zhu Z, Lin J, Zhang S, Wang C. Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice. J Genet Genomics, 2009, 36: 117-123
[28]Xia J C, Wang Y P, Ma B T, Yin Z Q, Hao M, Kong D W, Li S G. Ultrastructure and gene mapping of the Albino mutant al12 in rice (Oryza sativa L.). Acta Genet Sin, 2006, 33: 1112-1119
[29]Ueguchi M, Fujisawa Y, Kobayashi M, Ashikari M, Iwasaki Y. Rice dwarf mutant d1, which is defective in the a subunit of the heterotrimeric G protein, affects gibberellin signal transduction. Proc Natl Acad Sci USA, 2000, 97: 11639-11643
[30]Lanahan M B, Ho T H. Slender barley: A constitutive gibberellin-response mutant. Planta, 1988, 175: 107-114
[31]Dobrev P I, Kaminek M. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. J Chromatogr A, 2002, 950: 21-29
[32]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4325
[33]Shen Y J, Jiang H, Jin J P, Zhang Z B, Xi B, He Y Y, Wang G, Wang C, Qian L, Li X, Yu Q B, Liu H J, Chen D H, Gao J H, Huang H, Shi T L, Yang Z N. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol, 2004, 135: 1198-1205
[34]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: a rapid method to detect markers in specific genomic regions by using segregation population. Proc Natl Acad Sci USA, 1991, 88: 9828-9832
[35]Takeda K. Internode elongation and dwarfism in some gramineous plants. Gamma Field Sym, 1977, 17: 1-18
[36]Wang G, Römheld V, Li C, Bangerth F. Involvement of auxin and CKs in boron deficiency induced changes in apicak dominance of pea plants. J Plant Physiol, 2006, 163: 591-600
[37]Ekamber K P, Kumar M. Hormonal regulation of tiller dynamics in differentially-tillering rice cultivars. Plant Growth Regul, 2007, 53: 215-223
[38]Wilhelm R. Growth retardants: Effects on gibberellin biosynthesis and other metabolic pathways. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51: 501-531
[39]Fujioka S, Yokota T. Biosynthesis and metabolism of brassinosteroids. Annu Rev Plant Biol, 2003, 54: 137-164
[40]Mitsunaga S, Tashiro T, Yamaguchi J. Identification and characterization of gibberellins-insensitive mutants selected from among dwarf mutants of rice. Theor Appl Genet, 1994, 87: 705-712
[41]Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. Defect in non-yellow coloring 3, an alpha/beta hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J, 2009, 59: 940-52
[42]Meskauskiene R, Nater M, Goslings D, Kessler F, Camp R, Klaus K. FLU: A negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana. Proc Natl Acad Sci U S A, 2001, 98: 12826-12831
[43]Jeon J S, Lee S, Jung K H, Jun S H, Jeong D H, Lee J, Kim C, Jang S, Yang K, Nam J, An K, Han M J, Sung R J, Choi H S, Yu J H, Choi J H, Cho S Y, Cha S S, Kim S I, An G. T-DNA insertional mutagenesis for functional genomics in rice. Plant J, 2000, 22: 561-570
[44]Miyao A, Tanaka K, Murata K, Sawaki H, Takeda S, Abe K, Shinozuka Y, Onosato K, Hirochika H. Target site specificity of the Tos17 retrotransposon shows a preference for insertion within genes and against insertion in retrotransposon-rich regions of the genome. Plant Cell, 2003, 15: 1771-80
[45]Monde R A, Zito F, Olive J, Wollman F A, Stern D B. Post-transcriptional defects in tobacco chloroplast mutants lacking the cytochrome b6/f complex. Plant J, 2000, 21(1):61-72
[46]Kumar A M, Soll D. Antisense HEMA1 RNA expression inhibits heme and chlorophyll biosynthesis in Arabidopsis thaliana. Plant Physiol, 2000, 122: 49-55
[47]Xu Y Y, Jia J F, Wang B, Niu B T. Changes in isoenzymes and amino acids in forage and germination of the first post-flight generation of seeds of three legume species after space-flight. Grass Forage Sci, 1999, 54: 371-375
[48]Gomez-Roldan V, Fermas S, Brewer P B, Puech-Pagès V, Dun E A, Pillot J P, Letisse F, Matusova R, Danoun S, Portais J C, Bouwmeester H, Bécard G, Beveridge C A, Rameau C, Rochange S F. Strigolactone inhibition of shoot branching. Nature, 2008, 455: 189-194
[49]Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N, Magome H, Kamiya Y, Shirasu K, Yoneyama K, Kyozuka J, Yamaguchi S. Inhibition of shoot branching by new terpenoid plant hormones. Nature, 2008, 455: 195-200
[50]Xie X, Yoneyama K, Yoneyama K. The strigolactone story. Annu Rev Phytopathol. 2010, 48: 93-117
[51]Beveridge C A, Kyozuka J. New genes in the strigolactone-related shoot branching pathway. Curr Opin Plant Biol, 2010, 13: 34-39
[52]Bartley G E, Scolnik P A. Plant carotenoids: pigments for photoprotection, visual attraction, and human health. Plant Cell, 1995, 7: 1027-1038
[53]Tracewell C A, Vrettos J S, Bautista J A, Frank H A, Brudvig G W. Carotenoid photooxidation in photosystem II. Arch Biochem Biophys, 2001, 385: 61-69
[54]Wu D, Wright DA, Wetzel C, Voytas DF, Rodermel S. The IMMUTANS variegation locus of Arabidopsis defines a mitochondrial alternative oxidase homolog that functions during early chloroplast biogenesis. Plant Cell, 1999, 11: 43-55
[55]Carol P, Stevenson D, Bisanz C, Breitenbach J, Sandmann G, Mache R, Coupland G, Kuntz M. Mutations in the Arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. Plant Cell, 1999, 11: 57-68
[56]Aluru M, Yu F, Fu A, Rodermel S. Arabidopsis variegation mutants: new insights into chloroplast biogenesis. J Exp Bot, 2006, 57: 1871-1881
[57]Aluru M R, Rodermel S R. Control of chloroplast redox by the IMMUTANS terminal oxidase. Physiol Plant, 2004, 120: 4-11
[58]Josse E, Simkin A J, Gaffe J, Labourne A, Kuntz M, Carol P. A plastid terminal oxidase associated with carotenoid desaturation during chromoplast differentiation. Plant Physiol, 2000, 123: 1427-1436
[59]Akiyama K, Matsuzaki K, Hayashi H. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature, 2005, 435: 824-827
[60]Domagalska M A, Leyser O. Signal integration in the control of shoot branching. Nat Rev Mol Cell Biol, 2011, 12: 211-221
[61]Arnon D I, Allen M B, Whatley F R. Photosynthesis by Isolated Chloroplasts. Nature, 1954, 174: 394-396
[1] WANG Hao-Rang, ZHANG Yong, YU Chun-Miao, DONG Quan-Zhong, LI Wei-Wei, HU Kai-Feng, ZHANG Ming-Ming, XUE Hong, YANG Meng-Ping, SONG Ji-Ling, WANG Lei, YANG Xing-Yong, QIU Li-Juan. Fine mapping of yellow-green leaf gene (ygl2) in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(4): 791-800.
[2] TIAN Biao, DING Shi-Lin, LIU Chao-Lei, RUAN Ban-Pu, JIANG Hong-Zhen, GUO Rui, DONG Guo-Jun, HU Guang-Lian, GUO Long-Biao, QIAN Qian, GAO Zhen-Yu. Genetic analysis of seedling root traits and fine mapping of the QTL qLRL4 for the longest root length in rice [J]. Acta Agronomica Sinica, 2021, 47(10): 1863-1873.
[3] ZHOU Lian, LIU Chao-Xian, CHEN Qiu-Lan, WANG Wen-Qin, YAO Shun, ZHAO Zi-Kun, ZHU Si-Ying, HONG Xiang-De, XIONG Yu-Han, CAI Yi-Lin. Fine mapping and candidate gene analysis of maize defective kernel mutant dek54 [J]. Acta Agronomica Sinica, 2021, 47(10): 1903-1912.
[4] ZHANG Xue-Cui,ZHONG Chao,DUAN Can-Xing,SUN Su-Li,ZHU Zhen-Dong. Fine mapping of Phytophthora resistance gene RpsZheng in soybean cultivar Zheng 97196 [J]. Acta Agronomica Sinica, 2020, 46(7): 997-1005.
[5] REN Meng-Meng, ZHANG Hong-Wei, WANG Jian-Hua, WANG Guo-Ying, ZHENG Jun. Fine mapping of a major QTL qMES20-10 associated with deep-seeding tolerance in maize and analysis of differentially expressed genes [J]. Acta Agronomica Sinica, 2020, 46(7): 1016-1024.
[6] Li-Ping QIN,Er-Fei DONG,Yang BAI,Lian ZHOU,Lan-Yang REN,Ren-Feng ZHANG,Chao-Xian LIU,Yi-Lin CAI. Genetic analysis and molecular characterization of tasselseed mutant ts12 in maize [J]. Acta Agronomica Sinica, 2020, 46(5): 690-699.
[7] Xin-Ran SONG, Shu-Ting HU, Kai ZHANG, Ze-Jin CUI, Jian-Sheng LI, Xiao-Hong YANG, Guang-Hong BAI. Phenotypic analysis and fine mapping of dek101 in maize [J]. Acta Agronomica Sinica, 2020, 46(12): 1831-1838.
[8] SUN Qi, ZHAO Zhi-Chao, ZHANG Jin-Hui, ZHANG Feng, CHENG Zhi-Jun, ZOU De-Tang. Genetic analysis and fine mapping of a sheathed panicle mutant sui2 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2020, 46(11): 1734-1742.
[9] Di JIN,Dong-Zhi WANG,Huan-Xue WANG,Run-Zhi LI,Shu-Lin CHEN,Wen-Long YANG,Ai-Min ZHANG,Dong-Cheng LIU,Ke-Hui ZHAN. Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat [J]. Acta Agronomica Sinica, 2019, 45(6): 807-817.
[10] Li-Na SHANG,Xin-Long CHEN,Sheng-Nan MI,Gang WEI,Ling WANG,Ya-Yi ZHANG,Ting LEI,Yong-Xin LIN,Lan-Jie HUANG,Mei-Dan ZHU,Nan WANG. Phenotypic identification and gene mapping of temperature-sensitive green- revertible albino mutant tsa2 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2019, 45(5): 662-675.
[11] Zhong-Xiang LIU,Mei YANG,Peng-Cheng YIN,Yu-Qian ZHOU,Hai-Jun HE,Fa-Zhan QIU. Fine Mapping and Genetic Effect Analysis of a Major QTL qPH3.2 Associated with Plant Height in Maize (Zea mays L.) [J]. Acta Agronomica Sinica, 2018, 44(9): 1357-1366.
[12] Xiang-Yi XIAO,Xue-Tao SHI,Hao-Wen SHENG,Jin-Ling LIU,Ying-Hui XIAO. Fine Mapping and Candidate Gene Analysis of Rice Blast Resistance Gene Pi47 [J]. Acta Agronomica Sinica, 2018, 44(7): 977-987.
[13] Bao-Yu LIU, Jun-Hua LIU, Dan DU, Meng YAN, Li-Yuan ZHENG, Xue WU, Xian-Chun SANG, Chang-Wei ZHANG. Identification and Gene Mapping of a Lesion Mimic Mutant spl34 in Rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2018, 44(03): 332-342.
[14] ZHANG Xiao-Qiong, WANG Xiao-Wen, TIAN Wei-Jiang, ZHANG Xiao-Bo, Sun Ying, LI Yang-Yang, Xie Jia, HE Guang-Hua,SANG Xian-Chun. LAZY1 Regulates the Development of Rice Leaf Angle through BR Pathway [J]. Acta Agron Sin, 2017, 43(12): 1767-1773.
[15] ZHONG Jie,WEN Pei-Zheng,SUN Zhi-Guang,XIAO Shi-Zhuo,HU Jin-Long,ZHANG Le,JIANG Ling,CHENG Xia-Nian,LIU Yu-Qiang,WAN Jian-Min. Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population [J]. Acta Agron Sin, 2017, 43(11): 1596-1602.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd