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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 2115-2121.doi: 10.3724/SP.J.1006.2012.02115

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Changes in Microbial Flora and Bacterial Physiological Group Diversity on Leaf Surface of Transgenic Bt Corn

TIAN Xiao-Yan1,2,ZHAO Lei2,ZHAO Hui1,2,SUN Hong-Wei1,LI Fan1,YAN Shi-Lei2,LU Xing-Bo1,*   

  1. 1 Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2 College of Life Science, Shandong Normal University, Jinan 250014, China
  • Received:2012-03-13 Revised:2012-07-05 Online:2012-11-12 Published:2012-09-10
  • Contact: 路兴波, E-mail: luxb99@sina.com, Tel: 0531-83178363

Abstract:

In order to evaluate the effect of transgenic maize on epiphytes, the transgenic Bt maize Mon810 and its parental non-transgenic maize were grown in field to study the quantitative changes of culturable microorganisms and the diversity of bacterial functional groups at different growth stages in 2010 and 2011. Although there were differences in the colony-forming unites of culturable bacteria, actinomycetes and fungi of phyllosphere between Bt maize and non-Bt maize in different growing periods and different years, the trend of annual differences was basically consistent at the same growth period:the quantity of microbes in seeding stage is fewest and reaches the peak in the late of growing period. Compared with the controls, transgenic Bt maize seemed to stimulate the reproduction of phyllosphere fungi. However, no significant effect on the populations of actinomycete was observed. Significant differences were detected for bacteria at jointing stage, trumpet stage, silking stage and milking stage. In 2010, the Shannon-Wiener index, Simpson index and evenness index of bacteria physiological groups were higher at seeding, jointing and full-ripe stage, but lower at trumpet stage, tasseling stage, silking stage and milk stage. In 2011, the population characteristic parameters of the microorganisms of Bt-corn are more than the ones of non-Bt corn in the whole growing period except trumpet stage. It may be concluded there was some difference in terms of impacts between transgenic maize and non-transgenic maize, while the main impacts on microbial community composition were likely due to different years and different growth periods.

Key words: Bt corn, Epiphytes, Diversity, Risk assessment

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