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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1319-1324.doi: 10.3724/SP.J.1006.2013.01319

• RESEARCH NOTES • Previous Articles     Next Articles

Effects of Betaine on Chloroplast Protective Enzymes and psbA Gene Expression in Wheat Seedlings under Drought Stress

HOU Peng-Fei1,MA Jun-Qing2,ZHAO Peng-Fei1,ZHANG Huan-Ling1,ZHAO Hui-Jie1,LIU Hua-Shan1,ZHAO Yi-Dan1,WANG Yue-Xia1,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Academy of Forestry, Zhengzhou 450008, China
  • Received:2012-12-03 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 汪月霞, E-mail: yxwang2100@126.com E-mail:hpf88111@163.com

Abstract:

With the purpose of revealing the effect of exogenous glycine betaine (GB) on wheat seedlings, Aikang 58 was used as the experimental material, which has the characteristics of high yield and drought resistance. The leaves were sprayed with 0.1, 1.0, and 10.0 mmol L−1 of GB during four-leaf stage, respectively, while the root was applied by 30% polyethylene glycol (PEG-6000) to simulate the drought environment. The effects of exogenous GB treatment on the parameters of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) activities, superoxide anion radicals () production rate, malondialdehyde (MDA), chlorophyll content, and relative water content (RWC)under drought stress were tested three days after treatment. Simultaneously, the relative transcriptional expression of psbA inchloroplast was determined by real time PCR assay. The results showed that drought stress obviously decreased the RWC and chlorophyll content of wheat leaves, reduced activities of SOD, CAT, and POD, increased MDA content and the production rate of reactive oxygen species, and suppressed psbA expression. Nevertheless, such stress reactions were alleviated by exogenous GB treatment, and this regulation effect showed a great correlation with GB concentration. These results indicated thatexogenous GB could remove excess reactive oxygen, and retard the decrease of RWC and chlorophyll content by regulating antioxidant enzyme activities in wheat chloroplast under drought stress, enhance the transcriptional level of psbA gene, accelerate the turnover of D1 protein in wheat chloroplast, and finally improve the drought resistance in wheat.

Key words: Triticum aestivum, Betaine, psbA gene, Drought stress, Chloroplast antioxidant enzyme

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