Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1360-1365.doi: 10.3724/SP.J.1006.2013.01360

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Aspergillus niger phyA2 Transgenic Maize on Utilization of Organic Phosphorus in Soil

HOU Wen-Tong1,YANG Li-Ping1,*,CHEN Ru-Mei2,ZHANG Shao-Jun2   

  1. 1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-06 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 杨俐苹, E-mail: yangliping@caas.cn, Tel: 010-82105030

Abstract:

Homozygous phyA2 transgenic maize lines at T9 generation and the corresponding generation of negative control were grown in low-phosphorus (P) soil to investigate the ability of maize plant to acquire P from organic sources. It showed that the soil phosphatase activity increased by 5.17% and 5.48%, the moderately labile organic P decreased by 16.2% and 28.2% respectively in phyA2 transgenic maize rhizosphere, and the P accumulation of transgenic plant increased significantly by 140% and 100% respectively as compared with a control plant line when supplied with phytate as P sources and without P-fertilizer. The plant growth of phyA2 transgenic maize was better improved than that of the negative control plant. These data indicated that we can improve the ability of plant to utilize soil organic P, the plant P accumulation and the plant growth by transferring Aspergillus niger phyA2 gene into maize.

Key words: Phytase gene (phyA2), Maize, Soil organic phosphorus, Plant phosphorus

[1]Dalal R C. Soil organic phosphorus. Adv Agron, 1977, 29: 83–117



[2]Turner B L, Paphazy M J, Haygarth P M, Mckelvie I D. Inositol phosphates in the environment. Philos Trans R Soc Lond B Biol Sci, 2002, 357: 449–469



[3]Li Q-K(李庆逵), Zhu Z-L(朱兆良), Yu T-R(于天仁). China's Agricultural Sustainable Development of the Fertilizer (中国农业可持续发展中的肥料问题). Nanchang: Jiangxi Science and Technology Press, 1998. pp 1–5 (in Chinese)



[4]Ullah A H J, Sethumadhavan K, Mullaney E J, Ziegelhoffer T, Philips S A. Cloned and expressed fungal phyA gene in alfalfa produces a stable phytase. Biochem Biophy Res Commun, 2002, 290: 1343–1348



[5]Pen J, Verwoerd T C, Vanparidon P A, Beudeker R F, Vandenelzen P J M, Geerse K, Vanderklis J D, Versteegh H A J, Vanooyen A J J, Hoekema A. Phytase containing transgenic seeds as a novel feed additive for improved phosphorus utilization. Nat Biotechnol, 1993, 11: 811–814



[6]Verwoerd T C, Paridon P A, Ooyen A J J, Lent J W M, Hoekema A, Pen J. Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves. Plant Physical, 1995, 109: 1199–1205



[7]Richardson A E, Hadobas P A, Hayes J E. Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. Plant J, 2001, 25: 641–649



[8]Han S-F(韩胜芳), Gu J-T(谷俊涛), Xiao K(肖凯). Improving organic phosphate utilization in transgenic white clover by overexpression of Aspergillus niger PhyA gene. Acta Agron Sin (作物学报), 2007, 33(2): 250–255 (in Chinese with English abstract)



[9]Li M, Osaki M, Honma M, Tadano T. Purification and characterisation of phytase induced in tomato roots under phosphorus deficient conditions. Soil Sci Plant Nutr, 1997, 43: 179–190



[10]Brinch P H, Olesen A, Rasmussen S K, Preben B H. Generation of transgenic wheat (Triticum aestivum L.) for constitutive accumulation of an Aspergillus phytase. Mol Breed, 2000, 6: 195–206



[11]Hong C Y, Cheng K J, Tseng T H, Wang C S, Liu L F, Yu S M. Production of two highly active bacterial phytases with broad pH optima in germinated transgenic rice seeds. Transgenic Res, 2004, 13: 29–39



[12]Fang X-P(方小平), Wang Z(王转), Chen R-M(陈茹梅), Li J(李均), Fan Y-L(范云六), Luo L-X(罗莉霞), Chen K-R(陈坤荣), Ren L(任莉). Transgenic Brassica napus growing with phytate as a sole phosphorus source. Acta Agron Sin(作物学报), 2010, 36(2): 228–232 (in Chinese with English abstract)



[13]Chen R, Xue G, Chen P, Yao B, Yang W Z, Ma Q L, Fan Y L, Zhao Z Y, Tarczynski M C, Shi J R. Transgenic maize plants expressing a fungal phytase gene. Transgenic Res, 2008, 17: 633–643



[14]George T S, Simpson R J, Hadobas P A, Richardson A E. Expression of a fungal phytase gene in Nicotiana tabacum improves phosphorus nutrition of plants grown in amended soil. Plant Biotechnol J, 2005, 3: 129–140



[15]Wang Y(王祎). The Utilization of Transgenic Cotton with PhyA Gene for Organic Phosphate in Soil. PhD Dissertation of Huazhong Agricultural University, 2009 (in Chinese with English abstract)



[16]Huang H(黄惠). Study on Soil Phosphorus Uptake and Utilization of Transgenic Canola. PhD Dissertation of Agricultural University of Hebei, 2011 (in Chinese with English abstract)



[17]Tomes D T. Direct DNA transfer into plant cell via microprojectile bombardment. In: Gamborg O L, Philipps G C, eds. Plant Cell Tissue and Organ Culture: Fundamental Methods. Berlin: Springer-Verlag Publisher, 1995. pp 197–213



[18]Mao D-R(毛达如). Plant Nutrition Research Methods (植物营养研究方法), 2nd edn. Beijing: China Agricultural University Press, 2005. pp 423–424 (in Chinese)



[19]Lu R-K(鲁如坤). Soil Agricultural Chemical Analysis Method(土壤农业化学分析方法). Beijing: China Agriculture Science and Technology Press, 2000. pp 166–315 (in Chinese)



[20]Tarafdar J C, Jungk A. Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Feral Soils, 1987, 3:199-204



[21]Gerloff G C, Gabelman W H. Genetic basis of inorganic plant nutrition. In: Lauchli A, Bieleski R L, eds. Encyclopediaof Plant Physiology (New series Vol 15B). Berlin: Springer-Verlag, 1983. pp 450–480



[22]Elliot G C, Lauchi A. Phosphorus efficiencyandphosphate-ironin-teraction in maize. Agron J, 1985, 77: 399–403



[23]Helal H M. Varietal differences in root phosphatase activity as related to the utilization of organic phosphates. Plant & Soil, 1990, 123: 161–163

[1] WANG Dan, ZHOU Bao-Yuan, MA Wei, GE Jun-Zhu, DING Zai-Song, LI Cong-Feng, ZHAO Ming. Characteristics of the annual distribution and utilization of climate resource for double maize cropping system in the middle reaches of Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(6): 1437-1450.
[2] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[3] CHEN Jing, REN Bai-Zhao, ZHAO Bin, LIU Peng, ZHANG Ji-Wang. Regulation of leaf-spraying glycine betaine on yield formation and antioxidation of summer maize sowed in different dates [J]. Acta Agronomica Sinica, 2022, 48(6): 1502-1515.
[4] SHAN Lu-Ying, LI Jun, LI Liang, ZHANG Li, WANG Hao-Qian, GAO Jia-Qi, WU Gang, WU Yu-Hua, ZHANG Xiu-Jie. Development of genetically modified maize (Zea mays L.) NK603 matrix reference materials [J]. Acta Agronomica Sinica, 2022, 48(5): 1059-1070.
[5] XU Jing, GAO Jing-Yang, LI Cheng-Cheng, SONG Yun-Xia, DONG Chao-Pei, WANG Zhao, LI Yun-Meng, LUAN Yi-Fan, CHEN Jia-Fa, ZHOU Zi-Jian, WU Jian-Yu. Overexpression of ZmCIPKHT enhances heat tolerance in plant [J]. Acta Agronomica Sinica, 2022, 48(4): 851-859.
[6] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[7] YAN Yu-Ting, SONG Qiu-Lai, YAN Chao, LIU Shuang, ZHANG Yu-Hui, TIAN Jing-Fen, DENG Yu-Xuan, MA Chun-Mei. Nitrogen accumulation and nitrogen substitution effect of maize under straw returning with continuous cropping [J]. Acta Agronomica Sinica, 2022, 48(4): 962-974.
[8] XU Ning-Kun, LI Bing, CHEN Xiao-Yan, WEI Ya-Kang, LIU Zi-Long, XUE Yong-Kang, CHEN Hong-Yu, WANG Gui-Feng. Genetic analysis and molecular characterization of a novel maize Bt2 gene mutant [J]. Acta Agronomica Sinica, 2022, 48(3): 572-579.
[9] SONG Shi-Qin, YANG Qing-Long, WANG Dan, LYU Yan-Jie, XU Wen-Hua, WEI Wen-Wen, LIU Xiao-Dan, YAO Fan-Yun, CAO Yu-Jun, WANG Yong-Jun, WANG Li-Chun. Relationship between seed morphology, storage substance and chilling tolerance during germination of dominant maize hybrids in Northeast China [J]. Acta Agronomica Sinica, 2022, 48(3): 726-738.
[10] QU Jian-Zhou, FENG Wen-Hao, ZHANG Xing-Hua, XU Shu-Tu, XUE Ji-Quan. Dissecting the genetic architecture of maize kernel size based on genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(2): 304-319.
[11] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[12] ZHANG Qian, HAN Ben-Gao, ZHANG Bo, SHENG Kai, LI Lan-Tao, WANG Yi-Lun. Reduced application and different combined applications of loss-control urea on summer maize yield and fertilizer efficiency improvement [J]. Acta Agronomica Sinica, 2022, 48(1): 180-192.
[13] YU Rui-Su, TIAN Xiao-Kang, LIU Bin-Bin, DUAN Ying-Xin, LI Ting, ZHANG Xiu-Ying, ZHANG Xing-Hua, HAO Yin-Chuan, LI Qin, XUE Ji-Quan, XU Shu-Tu. Dissecting the genetic architecture of lodging related traits by genome-wide association study and linkage analysis in maize [J]. Acta Agronomica Sinica, 2022, 48(1): 138-150.
[14] ZHAO Xue, ZHOU Shun-Li. Research progress on traits and assessment methods of stalk lodging resistance in maize [J]. Acta Agronomica Sinica, 2022, 48(1): 15-26.
[15] NIU Li, BAI Wen-Bo, LI Xia, DUAN Feng-Ying, HOU Peng, ZHAO Ru-Lang, WANG Yong-Hong, ZHAO Ming, LI Shao-Kun, SONG Ji-Qing, ZHOU Wen-Bin. Effects of plastic film mulching on leaf metabolic profiles of maize in the Loess Plateau with two planting densities [J]. Acta Agronomica Sinica, 2021, 47(8): 1551-1562.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!