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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1799-1805.doi: 10.3724/SP.J.1006.2013.01799


Genetic and Expression Analyses of Ef7-l, a Novel Ef7 Allele, in Rice

ZHAO Dong-Sheng,ZHANG Chang-Quan,GU Ming-Hong,LIU Qiao-Quan*   

  1. Jiangsu Key Laboratory for Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2013-02-21 Revised:2013-05-25 Online:2013-10-12 Published:2013-07-09
  • Contact: 刘巧泉, E-mail: qqliu@yzu.edu.cn


Ef7 controls rice heading date. In present study, the allele of Ef7, Ef7-l, was cloned from an indica rice cultivar Longtefu. Sequence analysis indicated that Ef7-l is a novel Ef7 allele and there are five amino acid substitutions in Ef7-l compared with its wild type from Nipponbare. Based on the sequence variation between Ef7-l and Ef7,we developed a near-isogenic line (NIL) CL63 with the background of japonica Nipponbare via marker-assisted selection. The heading date of the NIL CL63 was postponed forapproximate six days under long day (LD) condition compared with Nipponbare. In short day (SD) condition, there was no obvious difference in heading date between Nipponbare and CL63. Under the natural field at Yangzhou, the novel allele Ef7-l could result in significant increase of plant height and grain weight in CL63 compared with Nipponbare. The qRT-PCR analysis revealed that there was not significant difference in Ef7 gene expression level between Nipponbare and CL63 under both LD and SD conditions. But the Ef7-l allele could affect rice heading date by up-regulating the expression level of OsPHYB gene under LD condition.

Key words: Oryza sativa L., Heading date, Ef7-l, Allele, Expression analysis

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