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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (07): 1182-1189.doi: 10.3724/SP.J.1006.2014.01182

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Fine Mapping of an Early Senescent Leaf Mutant esl5 in Oryza sativa

SANG Xian-Chun,XU Fang-Fang,ZHU Xiao-Yan,XING Ya-Di,HE Pei-Long,ZHANG Chang-Wei, ANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops /Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Online:2014-07-12 Published:2014-07-12
  • Contact: 何光华, E-mail: hegh@swu.edu.cn E-mail:sangxianchun@163.com

Abstract:

Premature senility directly influences crop yield and quality in the production. Therefore, it is crucial to identify early senescent leaf mutants and then clone genes associated with senescence by map-based strategy, which is significant in the research of senescence mechanism. An early senescent leaf mutant esl5 has been discovered from the progeny of indica restorer line Jinhui10 with seeds treated by ethylmethane sulfonate, this paper performed the studies on its morphological identification, cytological observation, physiological analysis and gene mapping. The results showed that the esl5 demonstrated normal phenotype at the seedling stage, displayed yellow green color at the tillering stage, and appeared yellow and senescent leaf blades at booting stage. Cell structure ofthe esl5 was badly influenced, showing destroyed cell membranes, loose and irregular stroma lamella in the section of senescent leaves. Chlorophyll content and photosynthetic rate of the esl5 were significantly twice than those of the wild type. Besides of the lower activities of SOD and CAT, the higher contents of ·OH and H2O2 were detected in the esl5 than in the wild type. The growth period of esl5 delayed 20 days, seed number per panicle, filled-seed number and seed setting rate increased significantly, but 1000-seed weight increased significantly at compared with the wild type. Genetic analysis suggested that the mutant was controlled by a recessive nuclear gene, and the ESL5 was fine mapped on chromosome 3 between Indel markers Indel03-1 and Indel03-2 with 83.4 kb physical distance, containing 11 annotated genes. These results provide a foundation for the cloning of ESL5 by map-based strategy, which is essential in the research of senescence mechanism as well as molecular breeding in Oryza sativa.

Key words: Rice (Oryza sativa), Early senescence, Gene mapping, Reactive oxygen species (ROS), Photosynthetic systems

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