Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1702-1709.doi: 10.3724/SP.J.1006.2014.01702

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles    

Molecular Cloning and Expression of a bZIP Transcription Factor Gene CsbZIP1 in Tea Plant (Camellia sinensis)

CAO Hong-Li,YUE Chuan,ZHOU Yan-Hua,WANG Lu,HAO Xin-Yuan,YANG Ya-Jun*,WANG Xin-Chao*   

  1. Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China?
  • Received:2014-01-08 Revised:2014-04-16 Online:2014-09-12 Published:2014-05-16
  • Contact: 王新超, E-mail: xcw75@tricaas.com, Tel: 0571-86653162; 杨亚军, E-mail: yjyang@tricaas.com, Tel: 0571-86650226

Abstract:

 

The basic leucine zipper proteins (bZIP) are one of the most extensive and conserved transcriptional factors families in eukaryotes, and plant bZIPs play important roles in many biological processes, especialy for resisting abiotic stresses. In this study, a bZIP full-length cDNA sequence was cloned using RACE and reverse transcription-PCR(RT-PCR) techniques from tea plant (Camellia sinensis). The obtained full-length cDNA was named CsbZIP1 with GenBank accession number JX050148.1. It is 1515 bp in length, containing a 813 bp open reading frame (ORF), encoding 270 amino acid residues with 29.484 kD molecular weight, and containing a typical BRLZ motif (basic region domain and leucine zipper domain) of B-zip1 family. The phylogenetic tree analysis revealed that CsbZIP1 belongs to F subfamily of bZIP. The subcellular location showed that CsbZIP1 protein is located in nucleus. The qRT-PCR analysis indicated that the expression level of CsbZIP1 was up-regulated by cold (4℃) and salt (NaCl) treatments, and both expression amounts increased at first, then declined after 24 hours. However, the expression pattern was down-regulated by ABA treatment within 24 hours. These results demonstrated that CsbZIP1 could be associated with cold and salt stresses in tea plant.

Key words: Tea plant (Camellia sinensis), bZIP transcription factor, CsbZIP1, Subcellular localization, Cloning and Expression

[1]Xu Z S, Chen M, Li L C, Ma Y Z. Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol, 2011, 53: 570–585



[2]Landschulz W, Johnson P, McKnight S. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science, 1988, 240: 1759–1764



[3]Kim S, Kang J Y, Cho D I, Park J H, Kim S Y. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J, 2004, 40: 75–87



[4]Jakoby M, Weisshaar B, Droge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F. bZIP transcription factors in Arabidopsis. Trends Plant Sci, 2002, 7: 106–111



[5]Nijhawan A, Jain M, Tyagi A K, Khurana J P. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol, 2008, 146: 333–350



[6]Gao S Q, Chen M, Xu Z S, Zhao C P, Li L, Xu H J, Tang Y M, Zhao X, Ma Y Z. The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants. Plant Mol Biol, 2011, 75: 537–553



[7]Rodriguez-Uribe L, O'Connell M A. A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris). J Exp Bot, 2006, 57: 1391–1398



[8]Liao Y, Zou H F, Wei W, Hao Y J, Tian A G, Huang J, Liu Y F, Zhang J S, Chen S Y. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta, 2008, 228: 225–240



[9]Liu C, Wu Y, Wang X. bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta, 2012, 235: 1157–1169



[10]Kang J Y. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell Online, 2002, 14: 343–357



[11]Ying S, Zhang D F, Fu J, Shi Y S, Song Y C, Wang T Y, Li Y. Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis. Planta, 2012, 235: 253–266



[12]Hsieh T H, Li C W, Su R C, Cheng C P, Sanjaya, Tsai Y C, Chan M T. A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. Planta, 2010, 231: 1459–1473



[13]Wang J, Zhou J, Zhang B, Vanitha J, Ramachandran S, Jiang S Y. Genome-wide expansion and expression divergence of the basic leucine zipper transcription factors in higher plants with an emphasis on sorghum. J Integr Plant Biol, 2011, 53: 212–231



[14]Lee S C, Choi H W, Hwang I S, Choi du S, Hwang B K. Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta, 2006, 224: 1209–1225



[15]周精华, 揭雨成, 邢虎成, 钟英丽, 余伟林. 苎麻BnbZIP1转录因子基因的克隆与表达特征分析. 中国农业科学, 2013, 46: 1314–1322



Zhou J H, Jie Y C, Xing H C, Zhong L Y, Yu W L. Cloning and characterization of the BnbZIP1 transcription factor gene from ramie (Boehmeria nivea L.). Sci Agric Sin, 2013, 46: 1314–1322 (in Chinese with English abstract)



[16]Wang Y, Gao C, Liang Y, Wang C, Yang C, Liu G. A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants. J Plant Physiol, 2010, 167: 222–230



[17]Chen H, Chen W, Zhou J, He H, Chen L, Chen H, Deng X W. Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice. Plant Sci, 2012, 193–194: 8–17



[18]Wang X C, Zhao Q Y, Ma C L, Zhang Z H, Cao H L, Kong Y M, Yue C, Hao X Y, Chen L, Ma J Q, Jin J Q, Li X, Yang Y J. Global transcriptome profiles of Camellia sinensis during cold acclimation. BMC Genomics, 2013, 14: 415



[19]Paul A, Lal L, Ahuja P S, Kumar S. Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep, 2012, 39: 3485–3490



[20]Wang Y, Jiang C J, Li Y Y, Wei C L, Deng W W. 2012. CsICE1 and CsCBF1: two transcription factors involved in cold responses in Camellia sinensis. Plant Cell Rep, 2012, 31: 27–34



[21]郝姗. 茶树不同逆境条件下QRT-PCR适宜内参基因的筛选. 南京农业大学硕士学位论文, 江苏南京, 2012. pp 38–65



Hao S. Selection of Appropriate Reference Genes for Expression Studies in Camellia sinensis by Real-time Polymerase Chain Reaction. MS Thesis of Nanjing Agricultural University, Nanjing, China, 2012. pp 38–65 (in Chinese with English abstract)



[22]曹红利, 岳川, 郝心愿, 王新超, 杨亚军. 茶树胆碱单加氧酶CsCMO的克隆及甜菜碱合成关键基因的表达分析. 中国农业科学, 2013, 46: 3087–3096



Cao H L, Yue C, Hao X Y, Wang X C, Yang Y J. Cloning of choline monooxygenase (CMO) gene and expression analysis of the key glycine betaine biosynthesis-related genes in tea plant (Camellia sinensis). Sci Agric Sin, 2013, 46: 3087–3096 (in Chinese with English abstract)



[23]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001, 25: 402–408



[24]Kumimoto R W, Siriwardana C L, Gayler K K, Risinger J R, Siefers N, Holt B F. Nuclear factor Y transcription factors have both opposing and additive roles in ABA-mediated seed germination. PLoS One, 2013, 8: e59481



[25]Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA, 2000, 97: 11632–11637



[26]Yamaguchi-Shinozaki K, Shinozaki K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol, 2006, 57: 781–803



[27]Wang B, Zheng J, Liu Y, Wang J, Wang G. Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize. Mol Biol Rep, 2012, 39: 6319–6327



[28]Zou M, Guan Y, Ren H, Zhang F, Chen F. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance. Plant Mol Biol, 2008, 66: 675–683



[29]Orellana S, Yanez M, Espinoza A, Verdugo I, Gonzalez E, Ruiz-Lara S, Casaretto J A. The transcription factor SlAREB1 confers drought, salt stress tolerance and regulates biotic and abiotic stress-related genes in tomato. Plant Cell Environ, 2010, 33: 2191–2208



[30]Schlogl P S, Nogueira F T, Drummond R, Felix J M, De Rosa V E, Jr.Vicentini R, Leite A, Ulian E C, Menossi M. Identification of new ABA- and MEJA-activated sugarcane bZIP genes by data mining in the SUCEST database. Plant Cell Rep, 2008, 27: 335–345



[31]Cheng C, Yun K Y, Ressom H W, Mohanty B, Bajic V B, Jia Y, Yun S J, de los Reyes B G. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice. BMC Genomics, 2007, 8: 175

[1] FENG Ya, ZHU Xi, LUO Hong-Yu, LI Shi-Gui, ZHANG Ning, SI Huai-Jun. Functional analysis of StMAPK4 in response to low temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(4): 896-907.
[2] WANG Zhen, YAO Meng-Nan, ZHANG Xiao-Li, QU Cun-Min, LU Kun, LI Jia-Na, LIANG Ying. Prokaryotic expression, subcellular localization and yeast two-hybrid library screening of BnMAPK1 in B. napus [J]. Acta Agronomica Sinica, 2020, 46(9): 1312-1321.
[3] WANG Dan-Dan, LIU Hong-Juan, WANG Hong-Xia, ZHANG Peng, SHI Chun-Yu. Cloning and functional analysis of the sweet potato sucrose transporter IbSUT3 [J]. Acta Agronomica Sinica, 2020, 46(7): 1120-1127.
[4] HENG You-Qiang,YOU Xi-Long,WANG Yan. Pathogenesis-related protein gene SfPR1a from Salsola ferganica enhances the resistances to drought, salt and leaf spot disease in transgenic tobacco [J]. Acta Agronomica Sinica, 2020, 46(4): 503-512.
[5] LI Na-Na, LIU Ying, ZHANG Hao-Jie, WANG Lu, HAO Xin-Yuan, ZHANG Wei-Fu, WANG Yu-Chun, XIONG Fei, YANG Ya-Jun, WANG Xin-Chao. Promoter cloning and expression analysis of the hexokinase gene CsHXK2 in tea plant (Camellia sinensis) [J]. Acta Agronomica Sinica, 2020, 46(10): 1628-1638.
[6] Ling WANG,Feng LIU,Ming-Jian DAI,Ting-Ting SUN,Wei-Hua SU,Chun-Feng WANG,Xu ZHANG,Hua-Ying MAO,Ya-Chun SU,You-Xiong QUE. Cloning and Expression Characteristic Analysis of ScWRKY4 Gene in Sugarcane [J]. Acta Agronomica Sinica, 2018, 44(9): 1367-1379.
[7] Mao-Ni CHAO, Qing-Yu WEN, Zhi-Yong ZHANG, Gen-Hai HU, Jin-Bao ZHANG, Guo WANG, Qing-Lian WANG. Sequence Characteristics and Expression Analysis of Potassium Transporter Gene GhHAK5 in Upland Cotton (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2018, 44(02): 236-244.
[8] WANG Bo,CAO Hong-Li,HUANG Yu-Ting,HU Yu-Rong,QIAN Wen-Jun,HAO Xin-Yuan, WANG Lu,YANG Ya-Jun,WANG Xin-Chao. Cloning and Expression Analysis of Auxin Efflux Carrier Gene CsPIN3 in Tea Plant (Camellia sinensis) [J]. Acta Agron Sin, 2016, 42(01): 58-69.
[9] Lü Gao-Qiang,WU Xiang-Yang,WANG Xin-Yu. Cloning and Characterization of a Novel Gene Encoding Proline-Rich Protein in Sesame [J]. Acta Agron Sin, 2015, 41(12): 1810-1818.
[10] MA Chun-Lei,YAO Ming-Zhe,WANG Xin-Chao,JIN Ji-Qiang,MA Jian-Qiang,CHEN Liang. Cloning and Expression of Three Genes Involved in the Biosynthesis of Chlorophyll in Different Albescent Stages of “Baiye 1” [J]. Acta Agron Sin, 2015, 41(02): 240-250.
[11] WANG Ce,YANG Yan-Ge,Lü Wei-Tao,ZHOU Chun-Ju,SUN Dong-Mei,DENG Xin. Cloning, Expression, and Functional Analysis of an A Subfamily bZIP Transcription Factor Gene ZmbZIP81 in Maize [J]. Acta Agron Sin, 2014, 40(09): 1549-1556.
[12] HUO Dong-Ying,ZHENG Wei-Jun,LI Pan-Song,XU Zhao-Shi,ZHOU Yong-Bin,CHEN Ming,MA You-Zhi,MIN Dong-Hong,ZHANG Xiao-Hong. Identification, Classification, and Drought Response of F-box Gene Family in Foxtail Millet [J]. Acta Agron Sin, 2014, 40(09): 1585-1594.
[13] YANG Yan-Ge,Lü Wei-Tao,SUN Dong-Mei,LING Yi,DENG Xin. Database Mining, Bioinformatics, Cloning and Expression Analyses of D Subfamily bZIP Genes in Maize [J]. Acta Agron Sin, 2013, 39(12): 2115-2122.
[14] JIN Yi-Rong, BAI Yan, SONG Yu-Feng, ZHANG Liang, DONG Lian-Hong, LIU Chao-Ke, FENG Xiang-Guo, HU Xiao-Ming, WANG Qian, LIU Hao-Bao. Molecular Cloning and Expression Analysis of Potassium Channel Gene NKT6 in Nicotiana sylvestris [J]. Acta Agronomica Sinica, 2013, 39(09): 1602-1611.
[15] YUE Chuan,ZENG Jian-Ming,CAO Hong-Li,HAO Xin-Yuan,ZHANG Zhi-Fang,WANG Xin-Chao,YANG Ya-Jun. Cloning and Expression Analysis of Gibberellin Receptor Gene CsGID1a in Tea Plant (Camellia sinensis) [J]. Acta Agron Sin, 2013, 39(04): 599-608.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!