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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 599-608.doi: 10.3724/SP.J.1006.2013.00599

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of Gibberellin Receptor Gene CsGID1a in Tea Plant (Camellia sinensis)

YUE Chuan1,2,**,ZENG Jian-Ming1,**,CAO Hong-Li1,2,HAO Xin-Yuan1,3,ZHANG Zhi-Fang1,WANG Xin-Chao1,*,YANG Ya-Jun1,*   

  1. 1 National Center for Tea Improvement, Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Horticulture, Northwest A&F University, Yangling 712100, China
  • Received:2012-09-16 Revised:2012-12-11 Online:2013-04-12 Published:2013-01-28
  • Contact: 王新超, E-mail: xcw75@mail.tricaas.com, Tel: 0571-86653162; 杨亚军, E-mail: yyjang@mail.tricaas.com

Abstract:

GID1 (Gibberellin insensitive dwarf1), as the soluble gibberellin (GA) receptor in GA signaling pathway, plays the vital role in GA reactions. In this study, the homologous gene of GID1 was isolated with RT-PCR and RACE-PCR from tea plant (Camellia sinensis). The obtained cDNA sequence, named CsGID1a, had the full-length of 1 411 bp containing a 1 023 bp open reading frame (ORF), encoding 341 amino acid residues, and was submitted to GenBank with accession number JX235369. The bioinformatics characterization indicated that CsGID1a was a non-secretory protein without a signal peptide. The molecular weight and theoretic isoelectric point of CsGID1a are 38.53 kD and 5.62, respectively. CsGID1a was located in the nucleus, encoding a protein with one transmembrane domain. CsGID1a contained hormone sensitive lipsase family (HSL) conserved domains, HGG and GXSXG motif, and shared the plant carboxylesterase tertiary structure. Homologous alignment and phylogenetic tree showed that CsGID1a shared over 60% amino acid sequence similarity with that of other species, and had the highest similarity (87%) and the closest genetic relationship to Vitis vinifera. The real-time PCR analysis showed that the expression of CsGID1a was down-regulated by high concentration of GA3 (1.0×10–5 mol L–1) and reduced slowly during the treatment for five hours. The further experiments suggested that the expression of CsGID1a was also decreased in the process of bud sprouting. These results demonstrated that CsGID1a and GA could be associated with bud bursting in tea plant in spring.

Key words: Tea plant (Camellia sinensis), Gibberellin (GA), GID1, Expression analysis, Bud bursting

[1]Olszewski N, Sun T P, Gubler F. Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell, 2002, 14(Suppl): 61–80



[2]Ueguchi-Tanaka M, Matsuoka M. The perception of gibberellins: clues from receptor structure. Curr Opin Plant Biol, 2010, 13: 503–508



[3]Yue C(岳川), Zeng J-M(曾建明), Cao H-L(曹红利), Wang X-C(王新超), Zhang Z-F(章志芳). The gibberellins metabolism and signaling pathway in higher plant. Plant Physiol J (植物生理学报), 2012, 2: 118–128 (in Chinese with English abstract)



[4]Hedden P. Gibberellins close the lid. Nature, 2008, 456: 455–456



[5]Murase K, Hirano Y, Sun T P, Hakoshima T. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature, 2008, 456: 459–464



[6]Sun T P. Gibberellin-GID1-DELLA: a pivotal regulatory module for plant growth and development. Plant Physiol, 2010, 154: 567–570



[7]Griffiths J, Murase K, Rieu I, Zentella R, Zhang Z, Powers S J, Gong F, Phillips A L, Hedden P, Sun T P, Thomas S G. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis. Plant Cell, 2006, 18: 3399–3414



[8]Fu X D, Richards D E, Fleck B, Xie D X, Burton N, Harberd N P. The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates. Plant Cell, 2004, 16: 1406–1418



[9]Dill A, Thomas S G, Hu J, Steber C M, Sun T P. The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. Plant Cell, 2004, 16: 1392–1405



[10]Santner A, Li C V, Estelle M. Plant hormones are versatile chemical regulators of plant growth. Nat Chem Biol, 2009, 5: 301–307



[11]Kakkar R K, Nagar P K. Distribution and changes in endogenous polyamines during winter dormancy in tea (Camellia sinensis (L.) O. Kuntze). Plant Physiol, 1997, 151: 63–67



[12]Nagar P K, Kumar A. Changes in endogenous gibberellin activity during winter dormancy in tea (Camellia sinensis (L.) O. Kuntze). Acta Physiol Plant, 2000, 22: 439–443



[13]Gupta D, Bhardwaj R, Nagar P K, Kaur S. Isolation and characterization of brassinosteroides from leaves of Camellia sinensis (L.) O. Kuntze. Plant Growth Regul, 2004, 42: 97–100



[14]Wang X-C(王新超). Identification and Expression Analysis of Genes Associated with the Bud Dormancy and Its Release of Tea Plant (Camellia sinenesis). PhD Thesis of Chinese Academy of Agricultural Sciences, 2011 (in Chinese with English abstract)



[15]Wang X-C(王新超), Ma C-L(马春雷), Yang Y-J(杨亚军), Ma J-Q(马建强), Cao H-L(曹红利). cDNA cloning and expression analysis of cyclin-dependent kinase(CsCDK)gene in tea plant. Acta Hort Sin (园艺学报), 2012, 39(2): 333–342 (in Chinese with English abstract)



[16]Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011, 28: 2731–2739



[17]Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins M R, Appel R D, Bairoch A. Protein identification and analysis tools on the ExPASyserver. Walker J M ed. The Proteomics Protocols Handbook. New York: Humana Press, 2005. pp 571–607



[18]Bendtsen J D, Nielsen H, Heijne G, Brunak S. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol, 2004, 340: 783–795



[19]Geourjon C, Deléage G. SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments. Computer Appl Biosci, 1995, 11: 681–684



[20]Cour T, Kiemer L, Molgaard A, Gupta R, Skriver K, Brunak S. Analysis and prediction of leucine-rich nuclear export signals. Protein Engin Design Select, 2004, 17: 527–536



[21]Arnold K, Bordoli L, Kopp J, Schwede T. The SWISS-MODEL Workspace: A web-based environment for protein structure homology modeling. Bioinformatics, 2006, 22: 195–201



[22]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 2001, 25: 402-408



[23]Ueguchi-Tanaka M, Nakajima M, Matsuoka M. Gibberellin receptor and its role in gibberellin signaling in plants. Annual Rev Plant Biol, 2007, 58: 183–198



[24]Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Yi, Kitano H, Yamaguchi I, Matsuoka M. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437: 693–698



[25]Østerlund T. Structure-function relationships of hormone-sensitive lipase. Eur J Biochem, 2001, 268: 1899–1907



[26]Ileperuma N R, Marshall S D, Squire C J, Squire J C, Baker H M, Oakeshott J G, Russell R J, Plummer K M, Newcomb R D, Baker E N. High-resolution crystal structure of plant carboxylesterase AeCXE1, from Actinidia eriantha, and its complex with a high-affinity inhibitor paraoxon. Biochemistry, 2007, 46: 1851–1859



[27]Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M. Structural basis for gibberellin recognition by its receptor GID1. Nature, 2008, 456: 520–524



[28]Nakajima M, Shimada A, Takashi Y, Kim Y C, Park S H, Ueguchi-Tanaka M, Suzuki H, Katoh E, Iuchi S, Kobayashi M, Maeda T, Matsuoka M, Yamaguch I. Identification and characterization of Arabidopsis gibberellins Receptors. Plant J, 2006, 46: 880–889



[29]Voegele A, Linkies A, Muller K, Leubner-Metzger G. Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination. J Exp Bot, 2011, 62: 5131–5147



[30]Li H P, Wang Y, Li X C, Gao Y, Wang Z J, Zhao Y, Wang M L. A GA-insensitive dwarf mutant of Brassica napus L. correlated with mutation in pyrimidine box in the promoter of GID1. Mol Biol Rep, 2011, 38: 191–197



[31]Mauriat M, Moritz T. Analyses of GA20ox- and GID1-over-expressing aspen suggest that gibberellins play two distinct roles in wood formation. Plant J, 2009, 58: 989–1003



[32]Willige B C, Ghosh S, Nill C, Dohmann E M N, Maier A, Schwechheimer C. The DELLA domain of GA INSENSITIVE mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin receptor of Arabidopsis. Plant Cell, 2007, 19: 1209–1220



[33]Iuchi S, Suzuki H, Kim Y C, Iuchi A, Kuromori T, Ueguchi-Tanaka M, Asami T, Yamaguchi I, Matsuoka M, Kobayashi M, Nakajima M. Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal. Plant J, 2007, 50: 958–966



[34]Ueguchi-Tanaka M, Hirona K, Hasegawa Y, Kitano H, Matsuoka M. Release of the repressive activity of rice DELLA protein SLR1 by gibberellin does not require SLR1 degradation in the gid2 mutant. Plant Cell, 2008, 20: 2437–2446



[35]Ariizumi T, Murase K, Sun T P, Steber C M. Proteolysis in dependent down regulation of DELLA repression in Arabidopsis by the gibberellin receptor GIBBERELLIN INSE NSITIVE DWARF 1. Plant Cell, 2008, 20: 2447–2459



[36]Yamamoto Y, Hirai T, Yamamoto E, Kawamura M, Sato T, Kitano H, Matsuoka M, Ueguchi-Tanaka M. A rice gid1 suppressor mutant reveals that gibberellin is not always required for interaction between its receptor, GID1, and DELLA proteins. Plant Cell, 2010, 11: 3589–602



[37]Gao Y, Chen J M, Zhao Y, Li T, Wang M. Molecular cloning and expression analysis of a RGA-like gene responsive to plant hormones in Brassica napus. Mol Biol Rep, 2012, 39: 1957–1962



[38]Hirano K, Nakajima M, Asano K, Nishiyama T, Sakakibara H, Kojima M, Katoh E, Xiang H, Tanahashi T, Hasebe M, Banks J A, Ashikari M, Kitano H, Ueguchi-Tanaka M, Matsuoka M. The GID1-mediated gibberellin perception mechanism is conserved in the Lycophyte selaginella moellendorffii but not in the Bryophyte Physcomitrella patens. Plant Cell, 2007, 19: 3058–3079



[39]Tyler L, Thomas S G, Hu J H, Dill A, Alonso J M, Ecker J R, Sun T P. DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant Physiol, 2004, 135: 1008–1019

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