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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (02): 187-196.doi: 10.3724/SP.J.1006.2015.00187


Identification of QTL Associated with Vitamin E Content in Soybean Seeds

ZHANG Hong-Mei1,LI Hai-Chao2,WEN Zi-Xiang2,GU He-Ping1,YUAN Xing-Xing1,CHEN Hua-Tao1,CUI Xiao-Yan1,CHEN Xin1,*,LU Wei-Guo2,*   

  1. 1 Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2 Institute of Industrial Crops, Henan Academy of Agricultural Scienecs / Zhengzhou Subcenter of National Center for Soybean Improvement / Key laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture, Zhengzhou 450002, China
  • Received:2014-06-25 Revised:2014-12-19 Online:2015-02-12 Published:2014-12-29
  • Contact: 陈新, E-mail: cx@jaas.ac.cn, Tel: 025-84391362; 卢为国, E-mail: 123bean@163.com, Tel. 0371-65733647 E-mail:zhm@ jaas.ac.cn


Vitamin E has effects on human immunity, anti-cancer and prevention of cardiovascular disease. Vitamin E from soybean has the advantages of higher security and higher human body absorption rate. The objective of the present study was to map the additive,additive × additive (epistasis), additive × year and epistasis × year QTLs for vitamin E and relative tocopherol contents with the RIL population BIEX (Essex×ZDD2315) using HPLC (high performance liquid chromatography) method and software QTLNetwork 2.1. Eight additive QTLs and twelve additive × additive (epistasis) QTLs were detected for vitamin E and relative tocopherol contents. The contributions to the phenotypic variances of additive QTL and epistatic QTL pairs were 8.68% (two QTLs) and 15.57% (four pairs) for α- tocopherol, 8.59% (two QTL) and 11.57% (two pairs) for γ-tocopherol, 5.44% (one QTL) and 17.61% (three pairs) for δ-tocopherol and 11.39% (three QTL) and 9.48% (three pairs) for total vitamin E contents, respectively. Those of additive and epistatic QTLs by year interaction were not found. The accumulated contribution of the unmapped minor QTLs was 66.16%–75.32%, indicating the variance of unmapped minor QTLs accounting for more than two thirds. In genetic composition, undetected minor QTLs accounted for a considerably large part additive QTLs and epistatic QTLs were nearly equal in α-tocopherol, γ-tocopherol, δ-tocopherol and total vitamin E contents. Accordingly, in breeding for vitamin E contents, the strategy of pyramiding multiple QTLs, both additive and epistatic, by using marker-assisted selection combined with accumulating minor effect QTLs through conversional procedures should be considered.

Key words: Soybean [Glycine max (L.) Merr.], Seed, Vitamin E content, QTL mapping

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